Purification, partial characterization, and serology of the capsid and cylindrical inclusion proteins of four isolates of watermelon mosaic virus / by Robert H. Baum.

Baum, Robert H.

doi:10.5962/bhl.title.42365

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…Detailed comparative studies of the host range, behaviour towards resistance genes in melons, and serological properties of coat protein, cylindrical inclusion protein, and amorphous inclusion protein of a large number of isolates of WMVMorocco, PRSV-W, ZYFV, ZYMV, and WMV 2 obtained from different countries showed that the isolates of WMV-Morocco were closely related to those of PRSV-W [3,24,25]. However, other investigations of biological properties, peptide mapping of protease V 8 digests in SDS-PAGE and serological relationships of coat protein and cylindrical inclusion protein using antisera to the Florida isoaltes of PRSV-W and WMV 2 suggested that WMV-Morocco is distinct from PRSV-W and WMV 2 [4][5][6]20].…”

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confidence: 99%

Watermelon mosaic virus-Morocco is a distinct potyvirus

McKern

Strike

Barnett

et al. 1993

Archives of Virology

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The relationship of the Morocco isolate of watermelon mosaic virus (WMV) to WMV2, soybean mosaic virus (a virus closely related to WMV2) and the W strain of papaya ringspot virus (PRSV-W), formerly WMV1, was examined by comparing tryptic peptide profiles using high performance liquid chromatography. The profiles indicated that the coat protein sequence of WMV-Morocco differed substantially from those of the other potyviruses. This conclusion was supported by sequence data from five tryptic peptides from the coat protein of WMV-Morocco, which showed only 61-68% identity to equivalent sequences in PRSV-W, WMV2 and zucchini yellow mosaic, another potyvirus infecting cucurbits. Based on the above data, and on known correlations between coat protein sequence similarities and potyvirus relationship, it is concluded that WMV-Morocco should be regarded as a distinct potyvirus.

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mentioning

confidence: 99%

Watermelon mosaic virus-Morocco is a distinct potyvirus

McKern

Strike

Barnett

et al. 1993

Archives of Virology

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Citrullus lanatus (Watermelon)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

et al. 2013

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Here we have identified and characterized a devastating virus capable of inducing yellow mosaic on the leaves of Patchouli [Pogostemon cablin (Blanco) Benth]. The diagnostic tools used were host range, transmission studies, cytopathology, electron microscopy, serology and partial coat protein (CP) gene sequencing. Evidence from biological, serological and sequence data suggested that the causal virus belonged to genus Potyvirus, family Potyviridae. The isolate, designated as Patchouli Yellow Mosaic Virus (PaYMV), was transmitted through grafting, sap and the insect Myzus persicae (Sulz.). Flexuous rod shaped particles with a mean length of 800 nm were consistently observed in leaf-dip preparations from natural as well as alternate hosts, and in purified preparation. Cytoplasmic cylindrical inclusions, pinwheels and laminar aggregates were observed in ultra-thin sections of infected patchouli leaves. The purified capsid protein has a relative mass of 43 kDa. Polyclonal antibodies were raised in rabbits against the coat protein separated on SDS – PAGE; which were used in ELISA and western blotting. Using specific antibodies in ELISA, PaYMV was frequently detected at patchouli plantations at Lucknow and Bengaluru. Potyvirus-specific degenerate primer pair (U335 and D335) had consistently amplified partial CP gene from crude preparations of infected tissues by reverse transcription polymerase chain reaction (RT-PCR). Comparison of the PCR product sequence (290 bp) with the corresponding regions of established potyviruses showed 78–82% and 91–95% sequence similarity at the nucleotide and amino acid levels, respectively. The results clearly established that the virus under study has close homology with watermelon mosaic virus (WMV) in the coat protein region and therefore could share a common ancestor family. Further studies are required to authenticate the identity of PaYMV as a distinct virus or as an isolate of WMV.

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Purification, partial characterization, and serology of the capsid and cylindrical inclusion proteins of four isolates of watermelon mosaic virus / by Robert H. Baum.

Cited by 3 publications

References 28 publications

Watermelon mosaic virus-Morocco is a distinct potyvirus

Watermelon mosaic virus-Morocco is a distinct potyvirus

Citrullus lanatus (Watermelon)

Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

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