Purification, Serology, and Vector Relationships of Squash Leaf Curl Virus, a Whitefly-Transmitted Geminivirus

Cohen, Shahar; Duffus, J. E.; Larsen, R.; Liu, H. Y.; Flock, R. A.

doi:10.1094/phyto-73-1669

Cited by 100 publications

(63 citation statements)

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“…This result was identical to the LP reported for a ToRMV isolate from Brasília, Brazil (Santos et al, 2003) and similar to the LP for CdTV, which was approximately 17 h (Brown & Nelson, 1988). The SLCV LP in B. tabaci biotype B was 19 h, while the LP for the TYLCV isolate from Jordan was 20 -24 h (Cohen et al, 1983;Mansour & Al-Musa, 1992), a little higher than the value obtained for ToYVSV. A LP longer than 24 h was found for the TYLCV isolate from Egypt (Mehta et al, 1994a), while a 6-h LP was reported for ToLCBV-C (Muniyappa et al, 2000).…”

Section: Resultssupporting

confidence: 87%

“…Results similar to the ones observed in the present study were obtained in the transmission of Cotton leaf curl virus form India (CLCuKV), Squash leaf curl virus (SLCV), Tomato leaf curl Bangalore virus -C (ToLCBV-C) (sin. Tomato leaf curl virus from Bangalore, India -ToLCVBan4), and Tomato yellow leaf curl virus (TYLCV-EG) isolate from Egypt by B. tabaci biotype B (Cohen et al, 1983;Nateshan et al, 1996;Muniyappa et al, 2000;Mehta et al, 1994a). A TYLCV isolate from Jordan had minimum acquisition and inoculation access periods of 60 and 30 minutes, respectively (Mansour & Al-Musa, 1992).…”

Section: Resultsmentioning

confidence: 99%

“…Sinaloa tomato leaf curl virus -STLCV) (Idris & Brown, 1998), 11 to 12 days for ToLCBV-C (Muniyappa et al 2000), 11 to 20 days for TYLCV isolate from Jordan (Cohen & Nitzany, 1966;Mansour & Al-Musa, 1992), and 26 days for SLCV (Cohen et al, 1983). These differences in virus AAP, IAP, LP, and period of retention within the insect are expected because, besides the variations in the conditions of the various experiments, especially with respect to insect numbers and the manner by which those insects were handled, other variables, such as begomovirus species, geographic origin of the virus isolate and of the B. tabaci biotype B population might affect these parameters, as reported in studies involving other begomoviruses (Picó et al, 1996;Muniyappa et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

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Tomato yellow vein streak virus: relationship with Bemisia tabaci biotype B and host range

Firmino¹,

Yuki²,

Moreira³

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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The Tomato yellow vein streak virus (ToYVSV) is a putative species of begomovirus, which was prevalent on tomato crops in São Paulo State, Brazil, until 2005. The objectives of this study were to evaluate the interaction between ToYVSV and its vector Bemisia tabaci biotype B and to identify alternative hosts for the virus. The minimum acquisition and inoculation access periods of ToYVSV by B. tabaci were 30 min and 10 min, respectively. Seventy five percent of tomato-test plants were infected when the acquisition and inoculation access periods were 24 h. The latent period of the virus in the insect was 16 h. The ToYVSV was retained by B. tabaci until 20 days after acquisition.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Tomato yellow vein streak virus: relationship with Bemisia tabaci biotype B and host range

Firmino¹,

Yuki²,

Moreira³

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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“…In early work, no serological relationship was detected between three geminiviruses, maize streak virus (MSV), African cassava mosaic virus (ACMV = cassava latent virus; Bock & Woods, 1983), and chloris striate mosaic virus (CSMV) : Francki et al, 1979. However, Sequeira & Harrison (1982) detected a serological relationship between ACMV and bean golden mosaic virus (BGMV), and Cohen et al (1983) found that ACMV is serologically related to squash leaf curl virus (SLCV). Similarly, tomato golden mosaic virus (TomGMV) was found to be serologically related to ACMV and BGMV (Stein et al, 1983) and, in addition, weak relationships were reported between beet curly top virus (BCTV) and both ACMV and TomGMV (Sequeira & Harrison, 1982;Stein etal., 1983).…”

Section: Introductionmentioning

confidence: 99%

Serological Relationships and Genome Homologies among Geminiviruses

Roberts¹,

Robinson²,

Harrison³

1984

Journal of General Virology

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SUMMARYIn immunosorbent electron microscopy (ISEM) tests, strong relationships were detected between five whitefly-transmitted geminiviruses: African cassava mosaic (ACMV), bean golden mosaic, euphorbia mosaic, squash leaf curl and tomato golden mosaic. Among five leafhopper-transmitted geminiviruses, beet curly top and tobacco yellow dwarf viruses were distantly related but no relationship was detected between either chloris striate mosaic, maize streak or wheat dwarf viruses and any of the other four. No relationship was detected between any whitefly-transmitted and any leafhopper-transmitted virus. A similar pattern of relationships was found by spot hybridization experiments in which extracts from infected leaves were tested with probes for ACMV DNA-1 or DNA-2. Imperfect nucleotide sequence homologies were found between ACMV DNA-1, which contains the particle protein gene, and the DNA of five other whitefly-transmitted viruses: bean golden mosaic, tomato golden mosaic, tobacco leaf curl, tomato leaf curl and tomato yellow leaf curl, the last three of which are not sap-transmissible. Thus, relationships were established between saptransmissible and sap non-transmissible geminiviruses. No homologies were detected with a full-length probe for ACMV DNA-2. Extracts from plants infected with three teafhopper-transmitted viruses (beet curly top, maize streak and wheat dwarf) did not react with probes for ACMV DNA-1 or DNA-2. Because each of the leafhoppertransmitted geminiviruses has a different vector species whereas the whiteflytransmitted geminiviruses all have the same vector, Bemisia tabaci, the genome homologies and antigenic relationships detected among members of the group could be explained if their coat proteins have a key role in transmission by vectors.

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“…As a result, transmission and purification of begomoviruses are difficult and yield of purified virus is usually low. However, tedious method of virus propagation in large number of host plants through whitefly (Bemisia tabaci) inoculation and purification of virus from large batches of infected plant tissues were performed to produce polyclonal antibodies (PAb) against a few begomoviruses such as squash leaf curl virus (SLCV) [3], tomato yellow leaf curl virus (TYLCV) [9] and tomato leaf curl Bangalore virus (ToLCBaV) [4]. The traditional method of begomovirus antigen preparation from infected plant tissue generally results into contamination with host proteins and thus limits their use in serological diagnosis.…”

Section: Abstract Pumpkin Yellow Vein Mosaic Virusmentioning

confidence: 99%

Fusion coat protein of pumpkin yellow vein mosaic virus with maltose binding protein: Applications in immunodiagnosis of begomoviruses

et al. 2014

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Availability of adequate quantity of purified virus preparation from plant tissue is the major limitation in producing polyclonal antibodies (PAb) to begomovirus. Very few examples show successful utilization of E. coli expressed recombinant coat protein (CP) for immuno diagnosis of begomoviruses. In the present study, *771 bp CP gene (*29.0 kDa) of Pumpkin yellow vein mosaic virus (PYVMV) was expressed as a *71.0 kDa fusion protein with maltose binding protein (MBP) (*42.0 kDa) in E. coli. The MBP-CP was obtained in soluble state. The PAb to the purified fusion protein successfully detected PYVMV and other bipartite and monopartite begomoviruses in the field samples at 1:250 dilution in enzyme linked immunosorbent assay. Our study for the first time showed that MBP-tag fusion CP was suitable to produce diagnostic antibody to begomoviruses. Keywords Pumpkin yellow vein mosaic virusBegomoviruses (family Geminiviridae) are emerging constrain in the production of numerous crops [14]. Both, immune-and nucleo-based techniques have been employed for the detection of begomoviruses [5]. Begomoviruses are phloem limiting, generally not sap transmissible and many of them do not have suitable propagative hosts. As a result, transmission and purification of begomoviruses are difficult and yield of purified virus is usually low. However, tedious method of virus propagation in large number of host plants through whitefly (Bemisia tabaci) inoculation and purification of virus from large batches of infected plant tissues were performed to produce polyclonal antibodies (PAb) against a few begomoviruses such as squash leaf curl virus (SLCV) [3], tomato yellow leaf curl virus (TYLCV) [9] and tomato leaf curl Bangalore virus (ToLCBaV) [4]. The traditional method of begomovirus antigen preparation from infected plant tissue generally results into contamination with host proteins and thus limits their use in serological diagnosis. Begomovirus purification by conventional method further limits the renewable production of PAb. As a result, routine detection and identification using serology has been less commonly used for begomoviruses compared to the other plant viruses. To overcome these limitations, molecular biology techniques are currently being used to express the viral genes of interest in E. coli and the recombinant antigen is used for the production of PAb. Recombinant antigens for several RNA plant viruses were successfully produced in E. coli [6,7,10], whereas, very few DNA plant viruses have been used for successful expression of recombinant antigen in E. coli [1].Pumpkin yellow vein mosaic virus, a bipartite begomovirus is known in India since 1950s [13] and the virus was characterized based on host range and serology [8]. Further, phylogenetic analysis of DNA-A sequence revealed that PYVMV was synonymous to squash leaf curl China virus (SLCCNV) reported from China [11,12]. There is an increasing incidence of PYVMV and many other begomoviruses in the Indian subcontinent [14], but PAb to begomoviruses for immunodia...

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Purification, Serology, and Vector Relationships of Squash Leaf Curl Virus, a Whitefly-Transmitted Geminivirus

Cited by 100 publications

References 0 publications

Tomato yellow vein streak virus: relationship with Bemisia tabaci biotype B and host range

Tomato yellow vein streak virus: relationship with Bemisia tabaci biotype B and host range

Serological Relationships and Genome Homologies among Geminiviruses

Fusion coat protein of pumpkin yellow vein mosaic virus with maltose binding protein: Applications in immunodiagnosis of begomoviruses

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