Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Abstract

The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant. O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column. The molecular weight was determined as 6.2 kDa. HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc. Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain. Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated. OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.

Polysaccharides fromBalanophora harlandiiHook: Isolation, Characterization, and Anti-Inflammation Activities

“…The literature has demonstrated the beneficial impact of glucans on inflammatory conditions (da Nascimento Santos et al, 2014; Li et al, 2017). Similar anti-inflammatory effects of 1, 4, 6-Glcp glycosidic bond have been reported previously (Hu et al, 2021). At present, polysaccharide activity is commonly believed to be greater in the β conformation.…”

Oligosaccharides fromAsparagus cochinchinensisfor ameliorating LPS-induced acute lung injury in mice

“…Previous studies have reported that saccharides, especially polysaccharides, from different plants played an important role in their anti-inflammatory properties. [25][26][27][28] However, their high molecular weight may negatively affect their beneficial effect, and for some polysaccharides, molecular degradation modification is required to enhance their activity by reducing their molecular weight to improve their bioavailability. 29 It is suggested that the natural oligosaccharides present in the plant have a lower molecular weight and greater solubility, foreshadowing that oligosaccharides may exhibit similar or even greater activity.…”

Evaluation of Anti-Obesity and Antidiabetic Activities of Orostachys japonicus in Cell and Animal Models

“…It contains many bioactive compounds such as organic acid, sugars, triterpenoids (glutinol, glutinone, friedelin, epi-friedelanol, β-amyrin, and taraxerone), triterpenes, phytosterols (β-sitosterol and campesterol), sterol glucosides, and flavonoids. Epicatechin gallate, quercetin, and kaempferol are known to be the major flavonoids found in the plant [ 13 , 14 ]. It is a widely used herb in various traditional medicine systems of China, Korea, and other Asian countries, and has been reported in various research articles for its promising biological activities [ 15 ].…”