Purification, structural characterization and in vitro antioxidant activity of a novel polysaccharide from Boshuzhi

Jiang, Jia-Yi; Kong, Fanrong; Li, Naisheng; Zhang, Dezhi; Yan, Chunyan; Hua-chong, LV

doi:10.1016/j.carbpol.2016.04.001

Cited by 112 publications

(36 citation statements)

References 36 publications

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“…It is well known that pro-inflammatory cytokines play a crucial role in avian respiratory disease progression, by coordinating and activating the adaptive immune response, which enables the host to eliminate pathogens [26]. Nii et al reported that the expression of pro-inflammatory cytokines was higher in the IBV-infected group than in the uninfected control group, suggesting that pro-inflammatory cytokines were involved in IBV progression [27].…”

Section: Discussionmentioning

confidence: 99%

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Zhang

Liu

et al. 2018

Microbial Pathogenesis

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The avian coronavirus causes infectious bronchitis (IB), which is one of the most serious diseases affecting the avian industry worldwide. However, there are no effective strategies for controlling the IB virus (IBV) at present. Therefore, development of novel antiviral treatment strategies is urgently required. As reported, astragalus polysaccharides (APS) have potential antiviral effects against several viruses; however, the antiviral effect of APS against IBV remains unclear. In this study, we explored whether APS had the potential to inhibit IBV infectionby utilizing several in vitro experimental approaches. To this end, the effect of APS on the replication of IBV was examined in chicken embryo kidney (CEK) cells. Viral titers were calculated by using the plaque formation assay, and the cytotoxicity of APS was tested by utilizing a Cell Counting Kit-8 assay. The expression of viral mRNA and cytokine (IL-1β, IL-6, IL-8 and TNF-α) mRNA transcripts was determined by real-time quantitative RT-PCR(qRT-PCR). IBV titers in infected CEK cells treated with APS were significantly reduced in a dose-dependent manner, indicating that APS inhibited IBV replication in vitro. We also found that the decreased viral replication after APS treatment was associated with reduced mRNA levels of the cytokines IL-1B, IL-6, IL-8 and TNF-α. In conclusion, these results suggest that APS exhibit antiviral activities against IBV and it may represent a potential therapeutic agent for inhibiting the replication of IBV.

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Section: Discussionmentioning

confidence: 99%

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Zhang

Liu

et al. 2018

Microbial Pathogenesis

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“…The relationship between the intrinsic viscosity η of the polymer solution and the molecular weight M of the polymer can be expressed by the empirical equation of Mark-Houwink: η = κM α , where κ and α are the two parameters of the empirical equation and are constants related to polymer morphology, solvent, and temperature. [21]. The four different molecular weight polysaccharide solutions (60 μg/mL, 1 mL) were incubated with phenanthroline (2.5 mmol/L, 1 mL), ferrous sulfate (2.5 mmol/L, 1 mL), and hydrogen peroxide (20 mmol/L, 1 mL) in phosphate buffer (20 mmol/L, 1 mL, pH 7.4) for 90 min at 37°C.…”

Section: Measurement Of Molecular Weights Of Polysaccharidesmentioning

confidence: 99%

Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products

Wang

Sun

Ouyang

2018

International Journal of Polymer Science

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To study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8.38 (APS1), 4.72 (APS2), and 2.60 kDa (APS3). The structures of these polysaccharides were characterized by 1H NMR, 13C NMR, FT-IR, and GC/MS. The degradation process did not cause significant changes in the main chain structure of APS. The monosaccharide component of APS before and after degradation was slightly changed. The antioxidant ability in vitro (removing hydroxyl and ABTS radicals and reducing ability) and in cells (superoxide dismutase and malondialdehyde generation) of these polysaccharides is closely related to their molecular weight. If the molecular weight of APS is very high or low, it is not conducive to their activity. Only APS2 with moderate molecular weight showed the greatest antioxidant activity and ability to repair human kidney epithelial (HK-2) cells. Therefore, APS2 can be used as a potential antistone polysaccharide drug.

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“…In the same concentration of sample, BOP showed stronger abilities for scavenging DPPH·, ·OH and ·O 2 − and chelating Fe 2+ than BSP [8]. Generally, the in vitro antioxidant activity of polysaccharides might be affected by multiple factors, such as molecular weight, configuration and even factors associated with the selected antioxidant evaluation system [36]. Thus, BOP exhibited better antioxidative capacity than BSP and should be developed as a potential natural antioxidant.…”

Section: Discussionmentioning

confidence: 97%

Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity

Wang

Chen

et al. 2020

Processes

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Bletilla ochracea Schltr. polysaccharides (BOP) have a similar structure to Bletilla striata (Thunb.) Reichb.f. (Orchidaceae) polysaccharides (BSP). Therefore, BOP can be considered as a substitute for BSP in the food, pharmaceuticals and cosmetics fields. To the best of our knowledge, little information is available regarding the optimization of extraction and antioxidant activity of BOP. In this study, response surface methodology (RSM) was firstly used for optimizing the extraction parameters of BOP. The results suggested that the optimal conditions included a temperature of 82 °C, a duration of 85 min and a liquid/material ratio of 30 mL/g. In these conditions, we received 26.45% ± 0.18% as the experimental yield. In addition, BOP exhibited strong concentration-dependent antioxidant abilities in vitro. The half-maximal effective concentration (EC50) values of BOP against 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonate) (ABTS+·), hydroxyl (·OH) and superoxide anion (·O2−) radicals and ferrous ions (Fe2+) were determined as 692.16, 224.09, 542.22, 600.53 and 515.70 µg/mL, respectively. In conclusion, our results indicate that BOP can be a potential natural antioxidant, deserving further investigation.

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Purification, structural characterization and in vitro antioxidant activity of a novel polysaccharide from Boshuzhi

Cited by 112 publications

References 36 publications

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products

Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity

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