Purine and Pyrimidine Nucleotide Synthesis and Metabolism

Moffatt, Barbara A.; Ashihara, Hiroshi

doi:10.1199/tab.0018

Cited by 275 publications

(270 citation statements)

References 114 publications

Supporting

Mentioning

266

Contrasting

Unclassified

Order By: Relevance

“…Substrate saturation kinetics was established for varying concentrations of [5,6-3 H]uracil and [2, H]adenine at 2.5 min using yeast cells (OD 600 = 4) expressing AtNCS1-1. Radiolabel uptake was assayed as mentioned above.…”

Section: Transport Kinetics Of Atncs1 Expressed In Yeastmentioning

confidence: 99%

“…A further trait in nucleobase biochemistry is the high degree of compartmentalization, necessitating extensive intra-and inter-cellular transport. A prime example is the essential interplay of nucleobase biochemical pathways in seed germination and early seedling development necessitating both intercellular transport of metabolites from endosperm to cotyledons as well as intracellular transport between organelles [1][2][3][4][5][6][7]. This highly involved nucleobase transport serves the numerous needs for nucleobases in plants and stands in contrast to nucleobase transport in microbes that mainly serves salvaging of external nitrogen sources.…”

Section: Introductionmentioning

confidence: 99%

“…Nucleic acid metabolism, carbohydrate, glycoprotein and phospholipid metabolism as well as the biosynthesis of many secondary metabolites, such as cytokinins, theobromine and caffeine, are just a few examples of the myriad of biochemistries dependent upon nucleobases [1][2][3][4]. Nucleobase biochemistry is marked by a sophisticated interaction between salvage reactions, de novo synthesis and catabolism pathways.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

a b s t r a c tLocus At5g03555 encodes a nucleobase cation symporter 1 (AtNCS1) in the Arabidopsis genome. Arabidopsis insertion mutants, AtNcs1-1 and AtNcs1-3, were used for in planta toxic nucleobase analog growth studies and radio-labeled nucleobase uptake assays to characterize solute transport specificities. These results correlate with similar growth and uptake studies of AtNCS1 expressed in Saccharomyces cerevisiae. Both in planta and heterologous expression studies in yeast revealed a unique solute transport profile for AtNCS1 in moving adenine, guanine and uracil. This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport).

show abstract

Section: Transport Kinetics Of Atncs1 Expressed In Yeastmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The nucleobases (purines and pyrimidines) are involved in nucleic acid metabolism; ATP synthesis; and carbohydrate, glycoprotein, and phospholipid metabolism (Ross 1991;Moffatt and Ashihara 2002;Stasolla et al 2003;Kafer et al 2004;Zrenner et al 2006). Secondary metabolites, such as caffeine and cytokinins, are nucleobase derivatives (Ashihara et al 2008;Frébort et al 2011).…”

Section: Introductionmentioning

confidence: 99%

The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility

et al. 2015

View full text Add to dashboard Cite

The solute specificity profiles (transport and binding) for the nucleobase cation symporter 1 (NCS1) proteins, from the closely related C4 grasses Zea mays and Setaria viridis, differ from that of Arabidopsis thaliana and Chlamydomonas reinhardtii NCS1. Solute specificity profiles for NCS1 from Z. mays (ZmNCS1) and S. viridis (SvNCS1) were determined through heterologous complementation studies in NCS1-deficient Saccharomyces cerevisiae strains. The four Viridiplantae NCS1 proteins transport the purines adenine and guanine, but unlike the dicot and algal NCS1, grass NCS1 proteins fail to transport the pyrimidine uracil. Despite the high level of amino acid sequence similarity, ZmNCS1 and SvNCS1 display distinct solute transport and recognition profiles. SvNCS1 transports adenine, guanine, hypoxanthine, cytosine, and allantoin and competitively binds xanthine and uric acid. ZmNCS1 transports adenine, guanine, and cytosine and competitively binds, 5-fluorocytosine, hypoxanthine, xanthine, and uric acid. The differences in grass NCS1 profiles are due to a limited number of amino acid alterations. These amino acid residues do not correspond to amino acids essential for overall solute and cation binding or solute transport, as previously identified in bacterial and fungal NCS1, but rather may represent residues involved in subtle solute discrimination. The data presented here reveal that within Viridiplantae, NCS1 proteins transport a broad range of nucleobase compounds and that the solute specificity profile varies with species.

show abstract

“…Durante o processo de catabolismo, purinas livres podem ser convertidas em ácido úrico e pirimidinas livres podem ser degradadas em compostos pequenos como β-aminoácidos [37].…”

Section: Quadro Geral Sobre O Metabolismo De Nucleotídeosunclassified

Estudo do mecanismo e inibição da enzima diidroorotato desidrogenase de Leishmania major

Reis¹

View full text Add to dashboard Cite

Purine and Pyrimidine Nucleotide Synthesis and Metabolism

Cited by 275 publications

References 114 publications

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility

Estudo do mecanismo e inibição da enzima diidroorotato desidrogenase de Leishmania major

Contact Info

Product

Resources

About