Purine and Pyrimidine Salvage Pathways

Nygaard, Per

doi:10.1128/9781555818388.ch26

Cited by 36 publications

(2 citation statements)

References 138 publications

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“…In contrast, nucleosides and nucleobases were one of the most utilized classes and used by all isolates. Presumably, this is via purine and pyrimidine salvage pathways which are known to be major pathways for bacteria to obtain carbon, energy, and nitrogen for growth ( Vogels and Van der Drift, 1976 ; Nygaard, 1993 ). Metabolite depletion patterns were similar for closely related isolates, although differences between the individual isolates can be observed ( Figure 2A ).…”

Section: Discussionmentioning

confidence: 99%

A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria

Raad

Kuehl

et al. 2022

Front. Microbiol.

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Exometabolomics is an approach to assess how microorganisms alter, or react to their environments through the depletion and production of metabolites. It allows the examination of how soil microbes transform the small molecule metabolites within their environment, which can be used to study resource competition and cross-feeding. This approach is most powerful when used with defined media that enable tracking of all metabolites. However, microbial growth media have traditionally been developed for the isolation and growth of microorganisms but not metabolite utilization profiling through Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Here, we describe the construction of a defined medium, the Northen Lab Defined Medium (NLDM), that not only supports the growth of diverse soil bacteria but also is defined and therefore suited for exometabolomic experiments. Metabolites included in NLDM were selected based on their presence in R2A medium and soil, elemental stoichiometry requirements, as well as knowledge of metabolite usage by different bacteria. We found that NLDM supported the growth of 108 of the 110 phylogenetically diverse (spanning 36 different families) soil bacterial isolates tested and all of its metabolites were trackable through LC–MS/MS analysis. These results demonstrate the viability and utility of the constructed NLDM medium for growing and characterizing diverse microbial isolates and communities.

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Section: Discussionmentioning

confidence: 99%

A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria

Raad

Kuehl

et al. 2022

Front. Microbiol.

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“…Deaminases are enzymes that induce the deamination of nucleotides and are related to salvage pathways of purines and pyrimidines ( Nygaard, 1993 ). Several deaminase domains have been predicted in polymorphic toxins ( Iyer et al ., 2011 ; Zhang et al ., 2012 ).…”

Section: Deaminasesmentioning

confidence: 99%

Intercepting biological messages: Antibacterial molecules targeting nucleic acids during interbacterial conflicts

et al. 2023

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Bacteria live in polymicrobial communities and constantly compete for resources. These organisms have evolved an array of antibacterial weapons to inhibit the growth or kill competitors. The arsenal comprises antibiotics, bacteriocins, and contact-dependent effectors that are either secreted in the medium or directly translocated into target cells. During bacterial antagonistic encounters, several cellular components important for life become a weak spot prone to an attack. Nucleic acids and the machinery responsible for their synthesis are well conserved across the tree of life. These molecules are part of the information flow in the central dogma of molecular biology and mediate long- and short-term storage for genetic information. The aim of this review is to summarize the diversity of antibacterial molecules that target nucleic acids during antagonistic interbacterial encounters and discuss their potential to promote the emergence antibiotic resistance.

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Pathways of Pyrimidine Salvage in Pseudomonas and Former Pseudomonas: Detection of Recycling Enzymes Using High-Performance Liquid Chromatography

Beck

O’Donovan

2007

Curr Microbiol

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Pyrimidine salvage pathways are vital for all bacteria in that they share in the synthesis of RNA with the biosynthetic pathway in pyrimidine prototrophs, while supplying all pyrimidine requirements in pyrimidine auxotrophs. Salvage enzymes that constitute the pyrimidine salvage pathways were studied in 13 members of Pseudomonas and former pseudomonads. Because it has been established that all Pseudomonas lack the enzyme uridine/cytidine kinase (Udk) and all contain uracil phosphoribosyl transferase (Upp), these two enzymes were not included in this experimental work. The enzymes assayed were: cytosine deaminase [Cod: cytosine + H2O --> uracil + NH3], cytidine deaminase [Cdd: cytidine + H2O --> uridine + NH3], uridine phosphorylase [Udp: uridine + Pi <--> uracil + ribose - 1 - P], nucleoside hydrolase [Nuh: purine/pyrimidine nucleoside + H2O --> purine/pyrimidine base + ribose], uridine hydrolase [Udh: uridine/cytidine + H2O --> uracil/cytosine + ribose]. The assay work generated five different Pyrimidine Salvage Groups (PSG) designated PSG1 - PSG5 based on the presence or absence of the five enzymes. These enzymes were assayed using reverse phase high-performance liquid chromatography techniques routinely carried out in our laboratory. Escherichia coli was included as a standard, which contains all seven of the above enzymes.

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Purine and Pyrimidine Salvage Pathways

Cited by 36 publications

References 138 publications

A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria

A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria

Intercepting biological messages: Antibacterial molecules targeting nucleic acids during interbacterial conflicts

Pathways of Pyrimidine Salvage in Pseudomonas and Former Pseudomonas: Detection of Recycling Enzymes Using High-Performance Liquid Chromatography

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