Purine nucleoside phosphorylase as a target to treat age-associated lower urinary tract dysfunction

Birder, Lori A.; Jackson, Edwin K.

doi:10.1038/s41585-022-00642-w

Cited by 5 publications

(3 citation statements)

References 78 publications

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“…PNP is essential for cell function and survival while its abnormal expression is closely related to the occurrence of various diseases. 67,68 In addition, PNP is a vital target for drug discovery and its inhibitors have been extensively investigated due to their promising uses as specic immunosuppressive, antiviral, and anticancer agents. 69 It is therefore highly desirable to design and develop facile methods to determine the activity of PNP as well as evaluate the efficiency of its inhibitor.…”

Section: Analysing and Visualizing Pnp Activity And Its Inhibitor Usi...mentioning

confidence: 99%

Enzyme-activatable charge transfer in gold nanoclusters

Deng,

Huang,

Zhong

et al. 2024

Chem. Sci.

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Section: Analysing and Visualizing Pnp Activity And Its Inhibitor Usi...mentioning

confidence: 99%

Enzyme-activatable charge transfer in gold nanoclusters

Deng,

Huang,

Zhong

et al. 2024

Chem. Sci.

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“…Imbalances in purine metabolism are implicated in disease: Deficiency in the enzyme purine nucleoside phosphorylase (PNPase) leads to complications with immunity and neurodevelopmental processes (Grunebaum et al, 2020 ). Uroprotective purines inosine, adenosine, and guanosine are converted to ROS‐producing hypoxanthine and xanthine by PNPase (Birder & Jackson, 2022 ). Inhibition of PNPase with the partial inhibitor 8‐aminoguanine (8‐AG) “rebalances” the purine metabolome, preventing the conversion of uroprotective to urotoxic purine metabolites and reduces subsequent ROS production.…”

Section: Considerations For Animal Modelsmentioning

confidence: 99%

Aging and urinary control: Alterations in the brain–bladder axis

Hardy,

Korstanje

2023

Aging Cell

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Age‐associated alterations in bladder control affect millions of older adults, with a heavy burden added to families both economically and in quality of life. Therapeutic options are limited with poor efficacy in older adults, lending to a growing need to address the gaps in our current understanding of urinary tract aging. This review summarizes the current knowledge of age‐associated alterations in the structure and function of the brain–bladder axis and identifies important gaps in the field that have yet to be addressed. Urinary aging is associated with decreased tissue responsiveness, decreased control over the voiding reflex, signaling dysfunction along the brain–bladder axis, and structural changes within the bladder wall. Studies are needed to improve our understanding of how age affects the brain–bladder axis and identify genetic targets that correlate with functional outcomes.

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“…For example, elevated levels of the protein Neural Growth Factor (NGF), produced by the bladder urothelium and bladder smooth muscle cells, may indicate renal dysfunction or bladder pathology [ 37 ]. Other examples include urinary markers related to smooth muscle stretch, such as urinary ATP, or specific metabolites, such as the purine derivative hypoxantin [ 38 ]. Many more biomarkers have meanwhile been described.…”

Section: Applications Of Biobanking In Neuro-urologymentioning

confidence: 99%

Neuro-Urology and Biobanking: An Integrated Approach for Advancing Research and Improving Patient Care

Botter,

Kessler

2023

IJMS

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Understanding the molecular mechanisms underlying neuro-urological disorders is crucial for the development of targeted therapeutic interventions. Through the establishment of comprehensive biobanks, researchers can collect and store various biological specimens, including urine, blood, tissue, and DNA samples, to study these mechanisms. In the context of neuro-urology, biobanking facilitates the identification of genetic variations, epigenetic modifications, and gene expression patterns associated with neurogenic lower urinary tract dysfunction. These conditions often present as symptoms of neurological diseases such as Alzheimer’s disease, multiple sclerosis, Parkinson’s disease, spinal cord injury, and many others. Biobanking of tissue specimens from such patients is essential to understand why these diseases cause the respective symptoms and what can be done to alleviate them. The utilization of high-throughput technologies, such as next-generation sequencing and gene expression profiling, enables researchers to explore the molecular landscape of these conditions in an unprecedented manner. The development of specific and reliable biomarkers resulting from these efforts may help in early detection, accurate diagnosis, and effective monitoring of neuro-urological conditions, leading to improved patient care and management. Furthermore, these biomarkers could potentially facilitate the monitoring of novel therapies currently under investigation in neuro-urological clinical trials. This comprehensive review explores the synergistic integration of neuro-urology and biobanking, with particular emphasis on the translation of biobanking approaches in molecular research in neuro-urology. We discuss the advantages of biobanking in neuro-urological studies, the types of specimens collected and their applications in translational research. Furthermore, we highlight the importance of standardization and quality assurance when collecting samples and discuss challenges that may compromise sample quality and impose limitations on their subsequent utilization. Finally, we give recommendations for sampling in multicenter studies, examine sustainability issues associated with biobanking, and provide future directions for this dynamic field.

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Purine nucleoside phosphorylase as a target to treat age-associated lower urinary tract dysfunction

Cited by 5 publications

References 78 publications

Enzyme-activatable charge transfer in gold nanoclusters

Enzyme-activatable charge transfer in gold nanoclusters

Aging and urinary control: Alterations in the brain–bladder axis

Neuro-Urology and Biobanking: An Integrated Approach for Advancing Research and Improving Patient Care

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