Purine Nucleotide Synthesis in Adrenal Chromaffin Cells

Rotllán, Pedro; Miras-Portugal, Marı́a Teresa

doi:10.1111/j.1471-4159.1985.tb08721.x

Cited by 22 publications

(2 citation statements)

References 40 publications

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“…When aden-osine was added to cells immediately after labeling there was a marked increase in the amount of [32P]ATP but no change in the turnover rate of [32P]ATP, indicating that the adenosine was rapidly incorporated into ATP. This is consistent with the report by Rotllan and Miras-Portugal (1985) and by unreported observations from our laboratory that [3H]adenosine added to cell cultures is rapidly incorporated into ATP. Thus, it appears likely that the specific activity of [3H]ATP of the total extravesicular pool is decreased by addition of adenosine.…”

Section: Discussionsupporting

confidence: 93%

Metabolic Pools of ATP in Cultured Bovine Adrenal Medullary Chromaffin Cells

Corcoran

Korner

Caughey

et al. 1986

Journal of Neurochemistry

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Cultured bovine adrenal chromaffin cells contain a pool of ATP sequestered within the chromaffin vesicles and an extravesicular pool of ATP. In a previous study it was shown that the turnover of ATP in the extravesicular pool was biphasic. One phase occurred with a t1/2 of 3.5–4.5 h whereas the second phase occurred with a t1/2 of several days. The studies described here were undertaken to characterize further the vesicular and extravesicular pools of ATP by examining the effects of metabolic inhibitors, adenosine, and digitonin on ATP utilization and subcellular localization immediately after and 48 h after labeling with [3H]adenosine and 32Pi. Immediately after labeling a combination of cyanide, 2‐deoxy‐D‐glucose, the β‐glucono‐l,5‐lactone resulted in a 90–95% depletion of the labeled ATP but only a 25% depletion of the endogenous ATP within 30 min. Forty‐eight hours after labeling, addition of the inhibitors resulted in a 70% depletion of the [3H]ATP but only a 25% depletion of the [32P]ATP and endogenous ATP. Addition of 10 μM adenosine to the media resulted in a similar loss of [3HJATP in cells examined immediately after or 48 h after labeling. Adenosine increased the amounts of [32P]ATP when added immediately after labeling but had no effect on the [32P]ATP content when added 48 h after labeling. Permeabilization of the plasma membrane by treatment with digitonin resulted in a loss of 90–95% of the labeled ATP but only 20% of the endogenous ATP in cells examined immediately after labeling and in a 79% loss of [3H]ATP, a 32% loss of [32P]ATP, and a 23% loss of endogenous ATP in cells examined 48 h after labeling. These studies show that ATP contained within chromaffin vesicles is metabolically inert and does not exchange readily with cytosolic ATP, but they do not differentiate extravesicular pools of labeled ATP. Calculation of ATP disappearance after abrupt cessation of ATP production indicates that cultured adrenal chromaffin cells utilize ATP at a rate of 0.24 nmol/min/106 cells, corresponding to a turnover time of 12.5 min for the extravesicular pool.

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Section: Discussionsupporting

confidence: 93%

Metabolic Pools of ATP in Cultured Bovine Adrenal Medullary Chromaffin Cells

Corcoran

Korner

Caughey

et al. 1986

Journal of Neurochemistry

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“…It was from the DBH of the chromaffin cells that María Teresa came into the purinergic field. Her initial interest was in purine metabolism, which led to the characterisation of adenine phosphoribosyltransferase, adenosine kinase [14,15], and the study of adenosine transport including its positive modulation by ATP [16][17][18][19][20][21]. These studies coincided with the description of the presence of diadenosine polyphosphates (Ap 4 A, Ap 5 A) in the chromaffin granules, from where they are released together with the classical nucleotides (ATP, ADP) and the other components of the "secretory cocktail" [22,23].…”

Section: From Dbh To Nucleotidesmentioning

confidence: 99%

María Teresa Miras Portugal: a pioneer in the study of purinoceptors in chromaffin cells

Artalejo

Arribas-Blázquez

Barahona

et al. 2023

Purinergic Signalling

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María Teresa Miras Portugal devoted most of her scientific life to the study of purinergic signalling. In an important part of her work, she used a model system: the chromaffin cells of the adrenal medulla. It was in these cells that she identified diadenosine polyphosphates, from which she proceeded to the study of adrenomedullary purinome: nucleotide synthesis and degradation, adenosine transport, nucleotide uptake into chromaffin granules, exocytotic release of nucleotides and autocrine regulation of chromaffin cell function via purinoceptors. This short review will focus on the current state of knowledge of the purinoceptors of adrenal chromaffin cells, a subject to which María Teresa made seminal contributions and which she continued to study until the end of her scientific life.

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Nucleotides

Grimble¹,

Westwood²

2000

Nutrition and Immunology

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Purine Nucleotide Synthesis in Adrenal Chromaffin Cells

Cited by 22 publications

References 40 publications

Metabolic Pools of ATP in Cultured Bovine Adrenal Medullary Chromaffin Cells

Metabolic Pools of ATP in Cultured Bovine Adrenal Medullary Chromaffin Cells

María Teresa Miras Portugal: a pioneer in the study of purinoceptors in chromaffin cells

Nucleotides

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