Purinergic signaling as a potential target of hypoxia stress-induced impairment of the immune system in freshwater catfish Lophiosilurus alexandri

Baldissera, Matheus D.; Souza, Carine F.; Boaventura, Túlio Pacheco; Nakayama, Cíntia Labussière; Baldisserotto, Bernardo; Luz, Ronald Kennedy

doi:10.1016/j.aquaculture.2018.07.025

Cited by 12 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, IL-6r is a pro-inflammatory cytokine essential for promoting the expression of proteins linked with acute inflammation [54]. Studies have indicated that catfish (Lophiosilurus alexandri) exhibit significantly higher plasma IL-6r levels than the control group during 24 and 72 h of hypoxic stress [55]. Ammonia stress in fish can trigger an inflammatory response, altering the expression of associated genes [14].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Acute Ammonia Nitrogen Stress on Antioxidant Ability, Phosphatases, and Related Gene Expression in the Kidney of Juvenile Yellowfin Tuna (Thunnus albacares)

Sun,

Fu,

2024

JMSE

View full text Add to dashboard Cite

This study investigated the effects of acute ammonia nitrogen (NH3-N) exposure on kidney antioxidant ability and phosphatases and related gene expression in juvenile yellowfin tuna (Thunnus albacares). The 180 juvenile yellowfin tuna (260.39 ± 55.99 g, 22.33 ± 2.28 cm) were exposed to ammonia for 6, 24, and 36 h using natural seawater (0 mg/L) as a control and NH3-N at 5 and 10 mg/L. The lipid peroxidation byproduct malondialdehyde (MDA) and the levels of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), alkaline phosphatase (AKP), and acid phosphatase (ACP), were measured using the colorimetric method in the trunk kidney to determine changes in antioxidant ability and phosphatase activity of juvenile yellowfin tuna exposed to NH3-N. Results indicated that, at 36 h, MDA, SOD, CAT, and GSH-PX levels rose in the 5 mg/L group versus the control. In the 10 mg/L group, MDA and SOD, CAT, and GSH-PX activities significantly increased after 24 and 36 h exposure compared to the control. Phosphatases play a pivotal role in the immune system. AKP activity significantly increased at 6 h, and ACP activity markedly rose at 36 h in the 5 mg/L group versus the control. Real-time fluorescence quantitative PCR was applied to detect alterations in the antioxidant genes SOD2, CAT, and glutathione peroxidase 1b (GPX1b) and immune cytokines-related genes Interleukin 10 (IL-10) and Interleukin 6 receptor (IL-6r) expression in the head kidney in juvenile tuna. Relative to the control, antioxidant gene expression in the 5 mg/L group significantly rose at 6 and 36 h, and in the 10 mg/L group, SOD2 and GPX1b were significantly elevated at 36 h. Compared to the control group, IL-10 expression in the 5 mg/L group significantly increased at 6 h, whereas IL-6r expression decreased. In the 10 mg/L group, both IL-10 and IL-6r levels were observed to be lower. Low ammonia nitrogen concentrations boost antioxidant defenses, phosphatase activities, and gene expression levels, whereas higher levels may induce suppressive effects. In yellowfin tuna juvenile farming, NH3-N concentration significantly affects the health of the juveniles. When the NH3-N concentration is between 5–10 mg/L, the stress duration should be limited to 24 h; if the concentration is below 5 mg/L, the stress duration can be extended to 36 h.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effects of Acute Ammonia Nitrogen Stress on Antioxidant Ability, Phosphatases, and Related Gene Expression in the Kidney of Juvenile Yellowfin Tuna (Thunnus albacares)

Sun,

Fu,

2024

JMSE

View full text Add to dashboard Cite

show abstract

“…In research conducted on hypoxia stress in Lophiosilurus alexandri, it was observed that ADA activity escalated following 24 and 72 h of hypoxia exposure in comparison to the group under normoxic conditions. Moreover, it is critical to underscore that the enzymatic activities of nucleoside triphosphate diphosphohydrolase (NTPDase) and ADA only reverted to their control levels following a recovery period of 72 h [63].…”

Section: Analysis Of Purine Metabolism Pathwaymentioning

confidence: 99%

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Zhang,

Wang,

Liu

et al. 2023

Water

View full text Add to dashboard Cite

In agricultural and fishery production, the occurrence of cyanobacterial blooms and the contamination of freshwater systems with microplastics have become increasingly important research focuses. However, the individual and combined toxic mechanisms of these two pollutants are not yet fully understood. Therefore, in this study, we analyzed the effects of these two stressors, microcystins (MC) and nanoplastics (NP), on the transcriptome and metabolome of the hepatopancreas of river clams. RNA and metabolites extracted from river clams treated with MC, NP, and a combination of MC and NP were used to construct standardized cDNA libraries, which were then subjected to integrated analysis. Significant enrichment of 49 pathways, 34 pathways, and 44 pathways was observed in the MC group compared to the control group, NP group compared to the control group, and NP-MC group compared to the control group, respectively. In these three experimental groups, we found that the lysosome pathway, which affects immune function and cell apoptosis, was enriched with numerous differentially expressed genes and metabolites. Changes in ATP6N and ADP may impair lysosomal acidification and disrupt normal lysosomal degradation processes, indicating interference with the hepatopancreatic metabolism of pollutants. Interestingly, we observed significant alterations in the cathepsin family, and the downregulation of cathepsin genes, along with the downregulation of ATP6N, implies a potential disruption in lysosomal proteolysis. In the NP-MC group, the downregulation of purine expression levels suggests an impact on the immune system of river clams by NP-MC. In conclusion, while there is some overlap in the damage caused to the hepatopancreas of river clams by MCs, MPs, and the combination of NP-MC, further research is necessary to fully understand their effects.

show abstract

“…Low oxygen levels are common in farming environments yet, according to Gilmore et al (2018), hypoxia can occur when dissolved oxygen (DO) in the water falls below a level physiologically suitable for sh, which affects their survival (Abdel-Tawwab et al 2019;Cook and Herbert 2012;Fitzgibbon et al 2007;Xiao 2015). Thus, hypoxia can impair sh growth (Abdel Tawwab et al 2019;Magnoni et al 2018) and reproduction (Konkal and Ganesh 2020;Lai et al 2019;Schreck 2010), as well as their immune system (Abdel Tawwab et al 2019;Baldissera et al 2018). In this sense, studies have revealed the effects of hypoxia on blood, biochemical and immunological parameters, which are good indicators of animals in inadequate conditions, for species such as Prochilodus nigricans (Val et al 2015), Micropterus salmoides (Yang et al 2017), Sparus aurata (Araújo-Luna et al 2018), Oreochromis niloticus (Li et al 2018) and Lophiosilurus alexandri (Baldissera et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, hypoxia can impair sh growth (Abdel Tawwab et al 2019;Magnoni et al 2018) and reproduction (Konkal and Ganesh 2020;Lai et al 2019;Schreck 2010), as well as their immune system (Abdel Tawwab et al 2019;Baldissera et al 2018). In this sense, studies have revealed the effects of hypoxia on blood, biochemical and immunological parameters, which are good indicators of animals in inadequate conditions, for species such as Prochilodus nigricans (Val et al 2015), Micropterus salmoides (Yang et al 2017), Sparus aurata (Araújo-Luna et al 2018), Oreochromis niloticus (Li et al 2018) and Lophiosilurus alexandri (Baldissera et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Lophiosilurus alexandri, a sedentary bottom fish, adjusts its physiological parameters to survive in hypoxia condition

Porto

Melo

Beier

et al. 2021

Fish Physiol Biochem

View full text Add to dashboard Cite

We investigated blood gas, hematological and biochemical parameters and gill morphology and morphometry of Lophiosilurus alexandri juveniles submitted to hypoxia for 48 hours followed by recovery for 48 hours. A total of 48 juveniles (360.0 ± 141.6 g) were distributed among eight tanks (120 L) and subjected to hypoxia condition (water with dissolved oxygen at 2.12 ± 0.90 mg L − 1 ) or normoxia (at 5.60 ± 0.31 mg L − 1 ). Blood gas values (pH, PvCO 2 , PvO 2 , sO 2 , HCO 3 − , stHCO 3 − and base excess) in hypoxia were signi cantly different from normoxia, while lactate and the electrolytes (K + , Na + , Cl − , Ca 2+ and HCO 3 − ) there was no signi cant change among treatments. The erythrocytes differed signi cantly between hypoxia and normoxia at 24 h of recovery, while for hemoglobin and hematocrit there were no signi cant differences. There was a signi cant difference in glucose, triglycerides, and cholesterol for both normoxia and hypoxia, while plasma protein remained unchanged. All gill components (epithelial cells, erythrocytes, pillar cells, mucus cells, chloride cells, undifferentiated cells, and blood capillary lumen) differed signi cantly between hypoxia and normoxia. A reduction in the length of the primary lamella was observed in the hypoxia and recovery treatments, when compared to normoxia. The secondary branchial lamella showed no signi cant difference for both treatments. In general, juveniles of L. alexandri adapted well to hypoxia exposure for 48 h, as they were able to adjust most of their physiological variables to survive this stress condition and return to normoxia within 48 h.

show abstract

Purinergic signaling as a potential target of hypoxia stress-induced impairment of the immune system in freshwater catfish Lophiosilurus alexandri

Cited by 12 publications

References 26 publications

The Effects of Acute Ammonia Nitrogen Stress on Antioxidant Ability, Phosphatases, and Related Gene Expression in the Kidney of Juvenile Yellowfin Tuna (Thunnus albacares)

The Effects of Acute Ammonia Nitrogen Stress on Antioxidant Ability, Phosphatases, and Related Gene Expression in the Kidney of Juvenile Yellowfin Tuna (Thunnus albacares)

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Lophiosilurus alexandri, a sedentary bottom fish, adjusts its physiological parameters to survive in hypoxia condition

Contact Info

Product

Resources

About