Purinergic Signaling Promotes Proliferation of Adult Mouse Subventricular Zone Cells

Suyama, Satoshi; Sunabori, Takehiko; Kanki, Hiroaki; Sawamoto, Kazunobu; Gachet, Christian; Koizumi, Schuichi; Okano, Hideyuki

doi:10.1523/jneurosci.4001-11.2012

Cited by 66 publications

(66 citation statements)

References 47 publications

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“…Recent studies have suggested that extracellular purinergic signaling also promotes the proliferation of NSCs in vitro [35,39] and in the adult SVZ [40]. However, whether ATP promotes the proliferation of NSCs in the adult DG and the sources of ATP remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Astrocytic Adenosine 5′-Triphosphate Release Regulates the Proliferation of Neural Stem Cells in the Adult Hippocampus

Cao

Qin

et al. 2013

Stem Cells

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Astrocytes are key components of the niche for neural stem cells (NSCs) in the adult hippocampus and play a vital role in regulating NSC proliferation and differentiation. However, the exact molecular mechanisms by which astrocytes modulate NSC proliferation have not been identified. Here, we identified adenosine 5 0 -triphosphate (ATP) as a proliferative factor required for astrocyte-mediated proliferation of NSCs in the adult hippocampus. Our results indicate that ATP is necessary and sufficient for astrocytes to promote NSC proliferation in vitro. The lack of inositol 1,4,5-trisphosphate receptor type 2 and transgenic blockage of vesicular gliotransmission induced deficient ATP release from astrocytes. This deficiency led to a dysfunction in NSC proliferation that could be rescued via the administration of exogenous ATP. Moreover, P2Y1-mediated purinergic signaling is involved in the astrocyte promotion of NSC proliferation. As adult hippocampal neurogenesis is potentially involved in major mood disorder, our results might offer mechanistic insights into this disease.

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Section: Discussionmentioning

confidence: 99%

Astrocytic Adenosine 5′-Triphosphate Release Regulates the Proliferation of Neural Stem Cells in the Adult Hippocampus

Cao

Qin

et al. 2013

Stem Cells

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“…The eyes were enucleated, the anterior segments were removed, and the posterior eyecups were immersion fixed for 15-30 min in 4 % paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4. After fixation, retinas were dissected from the eye cup, cryoprotected in graded sucrose solutions (10,20, and 30 %), and sectioned vertically at 14 μm on a cryostat. Sections were collected on Super-Frost glass slides and stored at −20°C until use.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…ATP released from neural precursor cells induces receptor-mediated Ca 2+ fluctuations in ventricular zone precursors of the developing brain and facilitates progenitor cell proliferation and migration within the developing cortex [7][8][9]. P2Y 1 receptor-mediated increase in progenitor cell proliferation has been shown in situ for both murine adult neurogenic niches, the subventricular zone (SVZ) of the lateral ventricles [10,11]), and the hippocampus [12]. The availability of extracellular nucleotides is controlled by ectonucleotidases, nucleotide-hydrolyzing enzymes bound to the cell surface.…”

Section: Introductionmentioning

confidence: 99%

NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation

Gampe

Haverkamp

Robson

et al. 2014

Purinergic Signalling

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Eye formation in vertebrates is controlled by a conserved pattern of molecular networks. Homeobox transcription factors are crucially involved in the establishment and maintenance of the retina. A previous study of Massé et al. (Nature, 449: 1058(Nature, 449: -62, 2007 using morpholino knockdown identified the ectonucleotidase NTPDase2 and the P2Y 1 receptor as essential elements for eye formation in embryos of the clawed frog Xenopus laevis. In order to investigate whether a similarly essential mechanism would be active in mammalian eye development, we analyzed mice KO for Entpd2 or P2ry1 as well as double KO for Entpd2/P2ry1. These mice developed normal eyes. In order to identify potential deficits in the molecular identity or in the arrangement of the cellular elements of the retina, we performed an immunohistological analysis using a variety of retinal markers. The analysis of single and double KO mice demonstrated that NTPDase2 and P2Y 1 receptors are not required for murine eye formation, as previously shown for eye development in Xenopus laevis.

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“…Fibroblast growth factor-2 (FGF-2) signalling promotes proliferation in both the SVZ and SGZ [37][38][39]. FGF-2 and TGF synthesis and secretion can be augmented by ATP, which can increase proliferation, and provide a potential explanation for the reduced neurogenesis in purinergic receptor knockout mice (P2Y1) [40,41]. Other factors also play a role in neurogenesis, including neuregulin-1, which has been implicated in dentate gyrus neurogenesis in addition to having antidepressant effects [42] and Growth Hormone (GH) which augments EGF and FGF2-induced proliferation [43].…”

Section: Proliferation and Neuronal Fatementioning

confidence: 99%

Regulation of Basal and Injury-Induced Fate Decisions of Adult Neural Precursor Cells: Focus on SOCS2 and Related Signalling Pathways

Basrai¹,

Christie²,

Turnley³

2013

Trends in Cell Signaling Pathways in Neuronal Fate Decision

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Purinergic Signaling Promotes Proliferation of Adult Mouse Subventricular Zone Cells

Cited by 66 publications

References 47 publications

Astrocytic Adenosine 5′-Triphosphate Release Regulates the Proliferation of Neural Stem Cells in the Adult Hippocampus

Astrocytic Adenosine 5′-Triphosphate Release Regulates the Proliferation of Neural Stem Cells in the Adult Hippocampus

NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation

Regulation of Basal and Injury-Induced Fate Decisions of Adult Neural Precursor Cells: Focus on SOCS2 and Related Signalling Pathways

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