Purity and yield of collagen extracted from southern catfish (Silurus meridionalis Chen) skin through improved pretreatment methods

Abstract

The economic and sustainable use of fish-processing by-products has received considerable attention. Large amounts of fish skin discarded by industry processes can be a source of collagen extraction. This study developed a method to extract collagen with high purity and yield from Southern catfish skins through improved pretreatment methods. Multiple degreasing methods effectively removed fat from fish skins and obtained a maximum degreasing rate (90.24%). The results of electrophoretic, hydroxyproline-content, and extraction-rate analyses revealed that the collagen extracted from defatted skin, which decoloured in 0.5% hydrogen peroxide solution (pH 10), exhibited higher purity and the highest yield (23.14% wet weight and 78.57% dry weight, respectively) as compared with other decolouration conditions. The histological appearance of pretreated fish skin indicated that the non-collagenous substances were removed effectively and the fibres loosened. Amino acid analysis, ultraviolet spectra, Fourier transform infrared (FTIR) spectroscopy, and thermal-stability analysis indicated that the collagen isolated from fish skin under optimal pretreated conditions was classified as type I. Our method for the optimal pretreatment of collagen extracted from fish skin following degreasing and decolouring procedures resulted in improved collagen purity and yield.

Preparation, characterization and in vitro wound healing activity of collagen-chitosan film

“…The intensity of the α1 was higher that of α2 which was much similar to the bands observed for the type 1 collagen from other ish species and the collagen from Catla catla species was con irmed to be type 1 collagen. Thus the yield of the collagen was obtained to be 9.28 % (Wet weight) which was similar to the Xu et al (2017) results. Similarly, Harati et al (2020) extracted collagen from tilapia, which showed good thermal and high solubility.…”

“…Extraction of collagen from ish skin was carried out following the protocol described by Xu et al (2017).…”

Factors affecting thermal stability of collagen from the aspects of extraction, processing and modification

“…Owing to the tight weave of collagen in tissues, some chemical reagents have been used to remove noncollagenous constituents and to loosen fiber contexture for extracting collagen easily [65,66]. Instead of traditional reagents, Ionic liquids (ILs) composed of bulky organic cations and small inorganic anions have been applied to the pretreatment of skin [67].…”

Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities

“…Importantly, however, these by-products are the main sources of structural and fibrous protein known as collagen which is the most abundant protein in mammals and the major constituent in connective tissues, skins and bones of animals. Collagen contents of the SPBs vary widely depending on the species, age, and season and can represent up 70% of dry weight for some specific species [68], e.g., 71% in leather jacket mince (skins, bones, and muscles) [95] or 78.6% in southern catfish (Silurus meridionalis Chen) skin [96]. Therefore, skins of different marine fish species such as bigeye snapper (Priacanthus macracanthus, Priacanthus tayenus, Priacanthus hamrur) [97][98][99] and cuttlefish (Sepia pharaonis) [100] have been utilised for extraction of collagen and its derivative gelatine, with the yield ranging from 10.9 to 33.2% on average.…”