Pursuing imazapyr herbicide tolerance in sugarcane: Screening plants produced in vitro through somaclonal variation and mutagenesis

Koch, A.; Ramgareeb, S.; Snyman, S. J.; Watt, M. P.; Rutherford, R. S.

doi:10.1016/j.sajb.2010.02.024

Cited by 6 publications

(9 citation statements)

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“…In P 1 significantly higher shoot length was recorded. Similar results were noticed by Kangna et al (2008) [10] , Koach et al (2009) [13] . and Chaudhari et al (2018) [4] .…”

Section: Effect Of Different Mutagenic Agents On Shoot Length (Cm)supporting

confidence: 90%

Deciphering in vitro mutated sugarcane clones for growth parameters

AJ¹,

VL²,

Rehevar³

et al. 2021

Pharma Innovation

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Callus derived from sugarcane variety CoN -13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 -30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, Maximum shoot length (26.6 cm) was observed in the treatment, EMS 0.2% + 30 min. Maximum root length (12.8 cm) was observed in treatment MMS 0.3% + 30 min. A higher concentration of all mutagenic agents registered higher root length with the maximum of 1.14 g was observed in treatment EMS 0.3% + 60 min. Maximum leaf area (44.7 cm 2 /plant) was observed in treatment MMS 0.2% + 30 min. Irrespective to concentration SA and EMS found significant for this character. Maximum chlorophyll content index (4.87) was observed in treatment EMS 0.2% + 30 min. In case of this character higher to lower concentration as well as time period influenced in both higher and lower directions. Among the treatments, the maximum multiplication rate (12.45%) was observed in treatment EMS 0.2% + 30 min. Both factors showed different effects on this character. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decreases with the increase in the time interval.

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“…In P 1 significantly higher shoot length was recorded. Similar results were noticed by Kangna et al (2008) [10] , Koach et al (2009) [13] . and Chaudhari et al (2018) [4] .…”

Section: Effect Of Different Mutagenic Agents On Shoot Length (Cm)supporting

confidence: 90%

Deciphering in vitro mutated sugarcane clones for growth parameters

AJ¹,

VL²,

Rehevar³

et al. 2021

Pharma Innovation

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show abstract

“…Higher concentration of SA (0.3%) at 60 min depicted poor survival per cent. Similar results were observed by Kanganal et al (2008) [11] , Koach et al (2009) [12] , Gadakh (2014) [8] and Chaudhari (2017) [6] .…”

Section: Effects Of Different Chemical Mutagenic Agents On Callus Survival Per Centsupporting

confidence: 88%

Genetic variability analysis in sugarcane (Saccharum spp. complex) through in vitro chemical mutagenesis on callus culture

AJ¹,

VL²,

Acharya³

et al. 2021

Pharma Innovation

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The present investigation on was carried out in south Gujarat heavy rainfall region of India. Callus derived from sugarcane variety CoN -13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 -30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, maximum survival per cent was observed in EMS 0.2% + 60 min treatment. Maximum regeneration per cent (78.2%) was found in explants treated with treatment MMS 0.3% + 30 min. In case of the number of days for shoot formation, callus treated with EMS 0.2% + 60 min registered a maximum of 15.20 days. The maximum numbers of multiple shoots were observed in MMS 0.2% + 60 min (30.4). Time factor found to be highly significant with a wide range of mean performance. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decrease with the increase in the time interval.

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“…Similar result noticed by Mallikarjun et al (2008) in sugarcane. Koch et al (2009) exposed callus to 8 mM and 16 mM EMS for development of tolerant cell.…”

Section: In Vitro Mutagenesismentioning

confidence: 99%

Evaluation of Differential Physiological and Biochemical Response of Sugarcane (Saccharum spp. Complex cv. Co 94012) Parent and Mutant’s in Response to Salt Stress

Gadakh¹,

Patel²,

Ban³

2017

IJBSM

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The present investigation carried out at Main Sugarcane Research Station, Navsari Agricultural University, Navsari during 2011−2014 to develop salt tolerance lines in sugarcane. Sugarcane mutants against salt tolerance were derived using in vitro mutagenesis and regeneration under NaCl stress. Sugarcane cv. 94012 was used for callus development from meristimatic leaf whorl on MS medium supplemented with 4 mg/l 2,4-D+2% sucrose. At treatment of Ethyl Methane Sulphonate (0.5%) for 2 hours treatment 48.60% calli survived similarly out of four concentration of NaCl 100 mM concentration of NaCl give at least 50.20% survived of calli. So these concentrations were used for development of salt tolerant mutant in sugarcane. The calli were treated with EMS lethal dose (LD 50) placed on shoot regeneration media containing LD 50 concentration of NaCl. The plants which regenerated from the tolerant calli were grown in pot culture system under five levels of salinity stress as compared to parental plants (source variety). With increasing supply of NaCl, root growth was more adversely affected. The growth of shoot, Chlorophyll content, photosynthesis rate, stomatal conductance and dry matter showed a decreasing trend but it shows significantly slow rate in tolerant mutants than parental plants. Mutant plants performed significantly well than parental plants in salt stress condition in all physiological and biochemical parameters. The result showed that high salt concentration Na + content in shoot of tolerant mutant and but was less than the normal plant. While, K + content in shoot was high in tolerant mutants than normal plants.

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Pursuing imazapyr herbicide tolerance in sugarcane: Screening plants produced in vitro through somaclonal variation and mutagenesis

Cited by 6 publications

References 0 publications

Deciphering in vitro mutated sugarcane clones for growth parameters

Deciphering in vitro mutated sugarcane clones for growth parameters

Genetic variability analysis in sugarcane (Saccharum spp. complex) through in vitro chemical mutagenesis on callus culture

Evaluation of Differential Physiological and Biochemical Response of Sugarcane (Saccharum spp. Complex cv. Co 94012) Parent and Mutant’s in Response to Salt Stress

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