Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

Moreno, Sergio Ruiz; Canals, Joan; Moro, Victor; Franch, Nil; Vilà, A.; Romano‐Rodríguez, A.; Prades, Joan Daniel; Bezshlyakh, Daria; Waag, A.; Kluczyk-Korch, Katarzyna; Maur, Matthias Auf der; Carlo, Aldo Di; Krieger, Sigurd; Geleff, Silvana; Diéguez, Ángel

doi:10.3390/s21103305

Cited by 6 publications

(5 citation statements)

References 53 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The setup is shown schematically in Figure 7, with the LED array on the bottom, the test pattern (in the figure a single metallic square) in the middle and the detector board on top. Details on the microscope can be found in [29,30].…”

Section: Resultsmentioning

confidence: 99%

Individually Switchable InGaN/GaN Nano-LED Arrays as Highly Resolved Illumination Engines

et al. 2021

Self Cite

View full text Add to dashboard Cite

GaN-based light emitting diodes (LEDs) have been shown to effectively operate down to nanoscale dimensions, which allows further downscaling the chip-based LED display technology from micro- to nanoscale. This brings up the question of what resolution limit of the illumination pattern can be obtained. We show two different approaches to achieve individually switchable nano-LED arrays. We evaluated both designs in terms of near-field spot size and optical crosstalk between neighboring pixels by using finite difference time domain (FDTD) simulations. The numerical results were compared with the performance data from a fabricated nano-LED array. The outcome underlines the influence of geometry of the LED array and materials used in contact lines on the final illumination spot size and shape.

show abstract

Section: Resultsmentioning

confidence: 99%

Individually Switchable InGaN/GaN Nano-LED Arrays as Highly Resolved Illumination Engines

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The resulting pixel density is 1411 PPI. The operation range of the pixel is between 0𝜇A and 120𝜇A, which makes the pixel viable to be integrated in microdisplays that are suitable for a wide range of applications, i. e. maskless photolithography [30], chip-based microscopy [31,32], or optogenetics [33].…”

Section: Discussionmentioning

confidence: 99%

17.1: SRAM‐based LED CMOS driver circuit for a 512x512 GaN microdisplay

Moro

Canals

Schöttler

et al. 2023

Symp Digest of Tech Papers

Self Cite

View full text Add to dashboard Cite

GaN microLED technology has the potential to offer displays with high brightness, bandwidth, and long lifetime with a very low energy consumption. Moreover, GaN‐on‐Si hybrid interconnection technology allows the development of displays with a very high pixel density integration. In this work we present an in‐pixel driving circuit designed in a 0.18µm CMOS technology to be integrated on a 512x512 microLED array by using the KlettWelding hybrid interconnection technique forming a microLED display of 1411 ppi with 10kfps working speed. The pixel driver is able to achieve switching times of 1MHz and can be operated at high bias currents of 120µA. The individual driver consists of 4 SRAMs that apply a weighted current and allows to avoid the current stability problems associated to CMOS backplanes in hybridly interconnected displays.

show abstract

“…Instead of scanning with a single LED, patterns of N x N LEDs are used to increase the excitation level of the fluorophore. Additionally, as demonstrated in [10] , oversampling is employed by using illumination patterns in the sample to maintain resolution.…”

Section: Overview Of Ultra-compact Microscope Componentsmentioning

confidence: 99%

“…In [9][10][11][12] , data acquisition from the CMOS camera and control of the microdisplay was carried out by software. However, this approach presents significant drawbacks in terms of speed.…”

Section: Hardware Accelerationmentioning

confidence: 99%

“…The validation process involved a single 10 µm SPAD detector from a 16x16 pixel SPAD CMOS camera designed on a 0.35 µm HV-CMOS process and an 8 × 8 Gallium Nitride (GaN) LED array. Moreno et al in [10] presented a compact microscope based on mechanical scanning. The microscope used a single 200 nm LED that emitted at a wavelength of 465 nm.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultra-compact fluorescence microscope for life-sciences

Moreno Martín,

Vilà,

Ramón Azcón

et al. 2024

Optical Instrument Science, Technology, and Applications III

Self Cite

View full text Add to dashboard Cite

Miniature microscopy provides a transformative approach to observe objects and enables continuous monitoring with ultra-compact microscopes attached directly to specimens, facilitating parallel analysis. This innovation is particularly valuable for applications such as drug discovery using organ-on-a-chip devices, which require the assessment of numerous drug/sample pairs prior to clinical trials. Ultra-compact microscopes were previously limited to brightfield techniques, which prevented the use of powerful tools like fluorescent microscopy. In this work, we present a miniature microscope with integrated fluorescence measurement capabilities. This microscope consists of a custom chip with a 10 μm-diameter single-photon avalanche diode (SPAD) faced to a 640 x 480 InGaN/GaN 4 µm-pitch LED microdisplay. It operates in raster mode, activating individual LEDs to map specimens in 2D while measuring fluorescence light with the SPAD chip. Our results demonstrate its suitability for life sciences imaging. For example, we observed a muscle-ona-chip stained with Alexa Fluor 488 to study drug efficacy on sarcopenia. Furthermore, these microscopes exhibit superior speed compared to the previously reported ultra-compact brightfield microscopes, achieving a 240-fold increase in imaging rate by means of hardware controller integration on FPGA.

show abstract

Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

Cited by 6 publications

References 53 publications

Individually Switchable InGaN/GaN Nano-LED Arrays as Highly Resolved Illumination Engines

Individually Switchable InGaN/GaN Nano-LED Arrays as Highly Resolved Illumination Engines

17.1: SRAM‐based LED CMOS driver circuit for a 512x512 GaN microdisplay

Ultra-compact fluorescence microscope for life-sciences

Contact Info

Product

Resources

About