Introduction: The current study aimed to assess the effect of non-thermal plasma (NTP) on the pushout bond strength (PBS) of epoxy resin and tricalcium silicate-based endodontic sealers. Methods: Forty single-canal extracted teeth were decoronated at the coronal region, underwent root canal preparation, and were assigned to four groups (n=10) for the application of AH26 sealer, NTP+AH26 (P-AH26), Endoseal TCS sealer, and NTP+Endoseal TCS sealer (P-TCS). The root canals were sectioned into 1 mm slices, and the PBS value was measured in a universal testing machine. Data were analyzed by the Friedman, Kruskal-Wallis, and Dunn tests (P<0.05). Results: The PBS of TCS and P-TCS groups was not significantly different (P>0.05). The PBS of the P-AH26 group was significantly lower than that of the AH26 group in the middle third (P<0.05). The PBS of the AH26 group was higher than the other groups in all sections. The PBS in the apical third was lower than other sections in all groups (P<0.05). Conclusion: NTP had no significant effect on the PBS of Endoseal TCS. NTP significantly decreased the PBS of AH26 sealer in the middle third but had no significant effect on its bond strength in other sections.