Putative role of intracellular Zn2+ release during oxidative stress: a trigger to restore cellular thiol content that is decreased by oxidative stress

Kinazaki, Akio; Chen, Hongqin; Koizumi, Kazuki; Kawanai, Takuya; Oyama, Tomohiro M.; Satoh, Masaya; Ishida, Shiro; Okano, Yoshiro; Oyama, Yasuo

doi:10.1007/s12576-011-0160-0

Cited by 32 publications

(25 citation statements)

References 48 publications

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“…In our study (Morita et al, 2012), we found that nanomolar concentrations (100-500 nM) of triclocarban significantly increased the intracellular Zn 2+ concentration in rat thymocytes. Intracellular Zn 2+ release, which results in an increase in the intracellular Zn 2+ concentration, occurs when cells are under oxidative stress (Matsui et al, 2010;Kinazaki et al, 2011). Therefore, there is a possibility that nanomolar concentrations of triclocarban additively increase the vulnerability of cells to oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

et al. 2013

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-It was recently reported that triclocarban was absorbed significantly from soap used during showering in human subjects and that its C max in their whole blood ranged from 23 nM to 530 nM. We revealed that a nanomolar concentration (300 nM) of triclocarban potentiated the cytotoxicity of 300 μM H 2 O 2 in rat thymocytes by using cytometric techniques with appropriate fluorescent probes. Although 300 nM triclocarban did not itself increase the population of dead cells (cell lethality), it facilitated the process of cell death induced by H 2 O 2 , resulting in a further increase in the population of dead cells. Nanomolar concentrations (300 nM or higher) of triclocarban significantly decreased the cellular content of nonprotein thiol (glutathione), which has a protective role against oxidative stress. Triclocarban at 300 nM or higher increased the cell vulnerability to oxidative stress. The results may suggest that nanomolar concentration (300 nM or higher) of triclocarban affects some cellular functions although there is no evidence for adverse effects of triclocarban in humans at present.

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Section: Introductionmentioning

confidence: 99%

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

et al. 2013

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“…1). The external application of ZnCl 2 increased intracellular Zn 2+ levels (Matsui et al, 2010;Kinazaki et al, 2011) and excessive increase in intracellular Zn 2+ level by ZnCl 2 was observed under the oxidative stress (Matsui et al, 2010). Excessive increase in intracellular Zn 2+ level (or zinc dyshomeostasis) induces oxidative stress (Kim et al, 1999;McCord and Aizenman, 2014).…”

Section: Possible Mechanism For Synergistic Action By H 2 O 2 and Dcoitmentioning

confidence: 99%

Zn2+-dependence of the synergistic increase in rat thymocyte cell lethality caused by simultaneous application of 4,5-dichloro-2-octyl-4-isothiazolin-3-one (DCOIT) and H2O2

et al. 2015

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“…However, increases in the intracellular Zn 2+ concentration elevate the cellular content of glutathione (Kinazaki et al, 2011). Therefore, the effect of IT-354 on 5-CMF fluorescence was examined.…”

Section: Changes In Oxonol and 5-cmf Fluorescence Induced By It-354mentioning

confidence: 99%

Cytotoxic actions of N-(2,4,6-trichlorophenyl)maleimide (IT-354), an antifouling agent, in rat thymic lymphocytes

Fukunaga

Saito

Kurumi

et al. 2015

Fundam. Toxicol. Sci.

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-Of antifoulants that are substitutes for organotin compounds such as tributyltin and triphenyltin, N-(2,4,6-trichlorophenyl)maleimide (IT-354) is listed as a much less toxic agent, although the available information concerning IT-354 toxicity is the results of acute toxicity tests in freshwater fish. In this study, the effects of IT-354 on rat thymic lymphocytes were examined using flow-cytometric techniques with appropriate fluorescent probes in order to estimate the effects of IT-354 on mammalian cells. Treatment of cells with 1-10 μM IT-354 for 1 hr did not increase the population of dead cells (cell lethality). However, 10 μM IT-354 significantly increased the population of living, annexin V-positive cells. Annexin V-positive, living cells are expected to be undergoing apoptosis. IT-354 at 3-10 μM significantly elevated intracellular Ca 2+ and Zn 2+ levels mainly by increasing Ca 2+ influx and intracellular Zn 2+ release. Furthermore, IT-354 significantly depolarized membranes and decreased cellular non-protein thiol content. Assessments using selected antifouling agents showed that the cellular actions of IT-354 are most likely similar to those of other commonly used antifouling agents. Therefore, the toxic potency of IT-354 on wild mammals is speculated to be similar to those of the other tested antifoulants.

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Putative role of intracellular Zn2+ release during oxidative stress: a trigger to restore cellular thiol content that is decreased by oxidative stress

Cited by 32 publications

References 48 publications

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

Zn2+-dependence of the synergistic increase in rat thymocyte cell lethality caused by simultaneous application of 4,5-dichloro-2-octyl-4-isothiazolin-3-one (DCOIT) and H2O2

Cytotoxic actions of N-(2,4,6-trichlorophenyl)maleimide (IT-354), an antifouling agent, in rat thymic lymphocytes

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