Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis: An Exploratory Study

Wang, Zhengyan; Pan, Jian; Wright, J. T.; Bencharit, Sompop; Zhang, Shaoping; Everett, Eric T.; Teixeira, Fabrício B.; Preisser, John S.

doi:10.1016/j.joen.2010.02.003

Cited by 79 publications

(83 citation statements)

References 31 publications

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“…Recent studies have reported the presence of viable stem cells in inflamed human dental pulps (Alongi et al 2010;Wang et al 2010;Pereira et al 2012). Our study also demonstrated that DPSCs isolated from teeth that were clinically diagnosed as IP preserved similar stem cell properties as DPSCs derived from healthy pulp, including morphology, colony-forming ; C, D) were seeded in the upper chamber.…”

Section: Discussionsupporting

confidence: 75%

“…This ablative approach is based on the generally accepted belief that once infection reaches the pulp, the infected and locally inflamed neurovascular structure may be irreversibly damaged with limited capability for repair. Recent studies reported that a subset of MSCs derived from inflamed human pulps preserve the full capability of proliferation and multipotent differentiation as compared with those from healthy pulps (DPSCs; Alongi et al 2010;Wang et al 2010;Pereira et al 2012), suggesting that inflamed pulp may not be completely depleted of progenitor/stem cells but can be repaired.…”

Section: Introductionmentioning

confidence: 99%

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DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

et al. 2016

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Human dental pulp stem cells (DPSCs) can be isolated from inflamed pulp derived from carious teeth with symptomatic irreversible pulpitis (I-DPSCs), which possess stemness and multidifferentiation potentials similar to DPSCs from healthy pulp. Since macrophagesessential cell players of the pulpal innate immunity-can regulate pulpal inflammation and repair, the authors investigated the immunomodulatory effects of DPSCs/I-DPSCs on macrophage functions and their underlying mechanisms. Similar to DPSCs, I-DPSCs were capable of colony-forming efficiency and adipogenic and osteo/dentinogenic differentiation under in vitro induction conditions. I-DPSCs also expressed a similar phenotypic profile of mesenchymal stem cell markers, except a relatively higher level of CD146 as compared with DPSCs. Coculture of DPSCs or I-DPSCs with differentiated THP-1 cells, the human monocyte cell line, markedly suppressed tumor necrosis factor α (TNF-α) secretion in response to stimulation with lipopolysaccharides (LPS) and/or nigericin. However, unlike TNF-α, the secreted level of interleukin 1β was not affected by coculture with DPSCs or I-DPSCs. Furthermore, DPSC/I-DPSC-mediated inhibition of TNF-α secretion by macrophages was abolished by pretreatment with 1-methyl-D-tryptophan, a specific inhibitor of indoleamine-pyrrole 2,3-dioxygenase (IDO), but not by NSC-398, a specific inhibitor of COX-2, suggesting IDO as a mediator. Interestingly, IDO expression was significantly augmented in macrophages and mesenchymal stromal cells in inflamed human pulp tissues. Collectively, these findings show that I-DPSCs, similar to DPSCs, possess stem cell properties and suppress macrophage functions via the TNF-α/IDO axis, thereby providing a physiologically relevant context for their innate immunomodulatory activity in the dental pulp and their capability for pulp repair.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

et al. 2016

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“…But MTA in its freshly mixed state shows a higher cytotoxicity [11,12] which could be due to its high pH [13]. Therefore, it is important to develop a new biocompatible material directed at maintaining a pulp vitality and increasing tooth longevity (Wang et al 2010) [14]. One such biologically based therapeutic is PRF.…”

Section: Discussionmentioning

confidence: 99%

PRF as a pulpotomy medicament in a permanent molar with pulpitis: a case report

Pathak¹,

Ahire²

2014

IOSRJDMS

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“…In current case the molar tooth exhibited signs and symptoms that of irreversible pulpitis, probably the coronal pulp, near minute carious lesion exposure site, was irreversibly inflamed and the radicular pulp remained vital with reversible pulpitis, however, the accurate histological diagnosis will remain uncertain for the case. Diseased pulp may not be completely damaged and some of the pulp cell might have capacity as stem cell potential, similar to healthy cells for autologous regeneration of pulp tissue [28].…”

Section: International Journal Of Dental Sciences and Researchmentioning

confidence: 99%

Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report

Aleid¹

2018

IJDSR

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Aim: management of a clinical case with irreversible pulpitis for young adult molar with closed apices using analogous blood derivative PRF and evaluating the clinical and radiographic success for coronal pulpotomy. Summary: A 18 years old male patient reported to the screening clinic, was referred to endodontic clinic with acute pulpitis diagnosed in left first molar tooth. Deep occlusal caries involving pulp, with history of spontaneous lingering pain associated with sign and symptoms of pulpitis. Standard clinical care protocol was strictly implemented, rubber dam isolation was followed by coronal pulpotomy. Blood drawn from cuboidal vein. Platelet rich fibrin membrane placed over the remaining pulp and restored with MTA and finally with Glass ionomer cement. Follow-up results clinically and radio-graphically were encouraging and successful at day 1 to 24 months. No postoperative pain, the treated tooth responded to pulp sensibility test and radiographs revealed periapical healing. However, for standardization of the clinical procedure well designed research study is further needed.

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Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis: An Exploratory Study

Cited by 79 publications

References 31 publications

DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

PRF as a pulpotomy medicament in a permanent molar with pulpitis: a case report

Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report

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