Putative TetR family transcriptional regulator Rv1019 ofMycobacterium tuberculosisis an auto-repressor and a negative regulator ofmfd-mazGoperon

Abstract

Putative TetR family transcriptional regulator Rv1019 of Mycobacterium tuberculosis is an 1 auto-repressor and a negative regulator of mfd-mazG operon 2 3 Abstract 16 Regulation of gene expression at the level of transcription is one of the ways living organisms 17 differentially make proteins in their cells. Intracellular pathogen Mycobacterium tuberculosis 18 persists in the host for years in a latent state in structures called granuloma. Rv1019, a member 19 of an uncharacterized TetR family of transcriptional regulators of Mtb H37Rv, was found to be 20 differentially expressed during dormancy and reactivation in vitro. By GFP reporter assays, 21 electrophoretic mobility shift assays and chromatin immuno-precipitation assays we showed 22 that this protein binds to its own promoter and acts as a negative regulator of its own expression. 23It forms dimers in vitro which is essential for the DNA binding activity, which is abrogated in 24 the presence of tetracycline. We show that Rv1019 and downstream genes Rv1020 (mfd), 25 Rv1021 (mazG) are co-transcribed. Constitutive expression of Rv1019 in M. smegmatis 26 downregulated MSMEG_5423 (mfd) and MSMEG_5422 (mazG) suggesting that Rv1019 27 negatively regulates these downstream genes. M. smegmatis expressing Rv1019 was found to 28 be sensitive to UV and H2O2 compared to the control suggesting its role in regulating DNA 29 damage response in mycobacterium. 30 Importance 31 One of the reasons for our inability to eradicate tuberculosis, in spite of the availability of 32 effective drugs and BCG vaccine, is the ingenuity of Mycobacterium tuberculosis to survive, 33 persist and multiply inside the lungs where it employs clever strategies to counter the host 34 defence systems. The number of anti-TB drugs in the current therapeutic regimen is limited, 35 and the long duration of treatment increases the chances for emergence of drug resistant 36 bacteria. Rv1019, a transcriptional regulator differentially expressed in in vitro dormancy-37 reactivation studies, was found to be an auto-repressor and negatively regulating mfd-mazG 38 operon involved in DNA repair. As Rv1019 is a regulator of critical pathways in bacterial 39 persistence, it could be a prospective target for prophylactic intervention.40