Putative virulence factors are released in association with membrane vesicles from<i>Burkholderia cepacia</i>

Allan, Nick; Kooi, Cora; Sokol, Pamela A.; Beveridge, Terry J.

doi:10.1139/w03-078

Cited by 46 publications

(27 citation statements)

References 47 publications

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“…We suggest that many of the changes in protein populations may result, at least in part, from the increased activity of an MV secretion system. This unique bacterial secretion system is used by many Gram-negative bacteria to combat host defense through delivery of large numbers of toxic components into host cells or into the extracellular milieu (15)(16)(17)(18). This secretion was specifically induced by physiological levels of glucose and was not observed in the closely related enteric Y. pseudotuberculosis.…”

Section: (C) Two (As-c-12 and Mf-73) Y Pestis Kim6mentioning

confidence: 99%

“…We suggest that the phenotype seen on the surface of glucose-grown bacteria is evidence of an MV phenomenon (47) capable of enhancing macromolecular secretion as described here. MVs are known to vary in size and biochemical properties depending on the bacterial genus, growth conditions, and lipopolysaccharide (LPS) component (15,18,48,49). Although Gram-negative bacterial MVs contain primarily periplasmic and outer membrane proteins, some are associated with cytoplasmic and inner membrane proteins, indicating that the vesicles can originate from the inner as well as the outer membrane and engulf whatever proteins are found there (50).…”

Section: (C) Two (As-c-12 and Mf-73) Y Pestis Kim6mentioning

confidence: 99%

“…Some of the features in the glucose-supplemented cultures (outer membrane blebbing, small spherical structures) resembled membrane vesicle (MV) secretion. The MV system has not previously been characterized in Y. pestis but is present in multiple pathogenic bacteria (15)(16)(17)(18). The induction of this system is related to bacterial stress responses and plays an important role in nutrient acquisition, biofilm development, and modulation of host defense mechanisms during progression of pathogenic lesions (16,17).…”

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confidence: 99%

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Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Protein Composition of Yersinia pestis Cell Surfaces

Kolodziejek

Caplan

Bohach

et al. 2013

Appl Environ Microbiol

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Section: (C) Two (As-c-12 and Mf-73) Y Pestis Kim6mentioning

confidence: 99%

Section: (C) Two (As-c-12 and Mf-73) Y Pestis Kim6mentioning

confidence: 99%

mentioning

confidence: 99%

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Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Protein Composition of Yersinia pestis Cell Surfaces

Kolodziejek

Caplan

Bohach

et al. 2013

Appl Environ Microbiol

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“…As MVs bleb from the outer membrane, periplasm fills their lumen and is retained there. The contents of the lumen vary according to the physiology of the parent organism and may consist of a variety of periplasmic constituents such as proteases, alkaline phosphatase, lipases, proelastase, autolysins, and toxins (2,20,25,31,34,36,39). Apart from the lumen contents, another important feature of MVs is their surface chemistry, which is similar to that of the outer membrane of the parent cell although differences in the relative stoichiometry do exist (30,34).…”

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confidence: 99%

Membrane Vesicles: an Overlooked Component of the Matrices of Biofilms

2006

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The matrix helps define the architecture and infrastructure of biofilms and also contributes to their resilient nature. Although many studies continue to define the properties of both gram-positive and gram-negative bacterial biofilms, there is still much to learn, especially about how structural characteristics help bridge the gap between the chemistry and physical aspects of the matrix. Here, we show that membrane vesicles (MVs), structures derived from the outer membrane of gram-negative bacteria, are a common particulate feature of the matrix of Pseudomonas aeruginosa biofilms. Biofilms grown using different model systems and growth conditions were shown to contain MVs when thin sectioned for transmission electron microscopy, and mechanically disrupted biofilms revealed MVs in association with intercellular material. MVs were also isolated from biofilms by employing techniques for matrix isolation and a modified MV isolation protocol. Together these observations verified the presence and frequency of MVs and indicated that MVs were a definite component of the matrix. Characterization of planktonic and biofilm-derived MVs revealed quantitative and qualitative differences between the two and indicated functional roles, such as proteolytic activity and binding of antibiotics. The ubiquity of MVs was supported by observations of biofilms from a variety of natural environments outside the laboratory and established MVs as common biofilm constituents. MVs appear to be important and relatively unacknowledged particulate components of the matrix of gram-negative or mixed bacterial biofilms.

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“…BCC strains also naturally release MVs and several virulence factors are contained in MVs (1). LPS from BCC is more toxic than that from P. aeruginosa (32,43).…”

Section: Pqs Induces MV Production In Gram-positive Bacteriamentioning

confidence: 99%

Pseudomonas Quinolone Signal Affects Membrane Vesicle Production in not only Gram-Negative but also Gram-Positive Bacteria

et al. 2010

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Many Gram-negative bacteria naturally produce membrane vesicles (MVs) to the extracellular milieu. The Pseudomonas quinolone signal (PQS), a quorum-sensing signal of Pseudomonas aeruginosa, is a positive regulator of MV production. In this study, we investigated its effects on MV production in other Gram-negative and -positive bacterial species. The addition of PQS to an Escherichia coli K12 culture resulted in increased MV production and enlarged MVs. An excessive amount of MgCl 2 repressed E. coli MV production either with or without PQS, suggesting that an anionic repulsion of cellular surfaces increases MV production. PQS was found in the cellular membrane and MVs in E. coli. The enhancement of MV production by PQS occurred in other Gram-negative bacteria, including Burkholderia and Pseudomonas species. Moreover, PQS induced MV production in a Gram-positive bacterium, Bacillus subtilis 168, which does not normally produce MV under laboratory conditions. An excessive amount of MgCl 2 did not repress B. subtilis MV production in the presence of PQS, suggesting the production mechanism to be different from that in Gram-negative bacteria. Together, these results indicated that PQS enhances MV production in Gram-negative bacteria and induces it in Gram-positive bacteria.

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Putative virulence factors are released in association with membrane vesicles fromBurkholderia cepacia

Cited by 46 publications

References 47 publications

Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Protein Composition of Yersinia pestis Cell Surfaces

Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Protein Composition of Yersinia pestis Cell Surfaces

Membrane Vesicles: an Overlooked Component of the Matrices of Biofilms

Pseudomonas Quinolone Signal Affects Membrane Vesicle Production in not only Gram-Negative but also Gram-Positive Bacteria

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