Putative virulence factors of Plesiomonas shigelloides

Edwards, Melissa S.; McLaughlin, Richard William; Li, Jia; Wan, Xiaoling; Liu, Yingli; Xie, Haixia; Hao, Yongsheng; Zheng, Jinsong

doi:10.1007/s10482-019-01303-6

Cited by 15 publications

(14 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, this is the first report of a P. shigelloides pathogen as a causative agent for enteritis in channel catfish. P. shigelloides is widely distributed in aquatic ecosystems and possesses a variety of virulence factors, including adhesive ability, hemolysins, cytotoxins, cholerae-like toxins and other exoenzymes associated with pathogenicity (Salerno et al, 2010;Edwards et al, 2019). Thus, P. shigelloides is a well-recognized fish opportunistic pathogen causing both intestinal and extra-intestinal diseases (Salerno et al, 2010;.…”

Section: Discussionmentioning

confidence: 99%

Plesiomonas shigelloides, a potential pathogen of enteritis in Ictalurus punctatus

Wang

Zhou

et al. 2020

Israeli Journal of Aquaculture - Bamidgeh

View full text Add to dashboard Cite

Enteritis has resulted in large economic losses in channel catfish aquaculture. Yet only scarce information is available on Plesiomonas shigelloides as a causal agent for this disease. In this study, a virulent strain, temporarily named BD1, was isolated from diseased channel catfish suffering from enteritis, and was identified as P. shigelloides through molecular and phenotypic methods. A phylogenetic tree was also constructed to determine its taxonomic position. In addition, the BD1 isolate has developed multiple resistances to aminoglycosides, amphenicols, quinolones, tetracyclines and sulfonamides drugs for veterinary uses in aquaculture as revealed when screened against a range of common antibiotics. To the best of our knowledge, this is the first report of P. shigelloides as a potential pathogen of enteritis in channel catfish. * Antibiotics for aquaculture use (Ministry of Agriculture of China, 2013).

show abstract

Section: Discussionmentioning

confidence: 99%

Plesiomonas shigelloides, a potential pathogen of enteritis in Ictalurus punctatus

Wang

Zhou

et al. 2020

Israeli Journal of Aquaculture - Bamidgeh

View full text Add to dashboard Cite

show abstract

“…In a study, Hurley et al performed WGS-based characterization of 100 Listeria monocytogenes strains to determine disease epidemiology, the presence of drug resistance genes, and to determine virulence factors [223]. Similarly, another study was conducted by Edwards et al to characterize the putative virulence factors in Plesiomonas shigelloides [228]. Furthermore, Aly et al used a WGS-based study to identify genes associated with biofilm formation, antibiotic resistance, and pathogenicity [224].…”

Section: Whole Genome Sequencing and Genome Wide Association Studimentioning

confidence: 99%

Helicobacter pylori Virulence Factors Exploiting Gastric Colonization and its Pathogenicity

Ansari

Yamaoka

2019

Toxins

189

141

View full text Add to dashboard Cite

Helicobacter pylori colonizes the gastric epithelial cells of at least half of the world’s population, and it is the strongest risk factor for developing gastric complications like chronic gastritis, ulcer diseases, and gastric cancer. To successfully colonize and establish a persistent infection, the bacteria must overcome harsh gastric conditions. H. pylori has a well-developed mechanism by which it can survive in a very acidic niche. Despite bacterial factors, gastric environmental factors and host genetic constituents together play a co-operative role for gastric pathogenicity. The virulence factors include bacterial colonization factors BabA, SabA, OipA, and HopQ, and the virulence factors necessary for gastric pathogenicity include the effector proteins like CagA, VacA, HtrA, and the outer membrane vesicles. Bacterial factors are considered more important. Here, we summarize the recent information to better understand several bacterial virulence factors and their role in the pathogenic mechanism.

show abstract

“…However, subsequent experiments proved that Plesiomonas shigelloides strain EE2 can be formed even without these proteins. This indicated that P. shigelloides uses other mechanisms to regulate the formation of biofilms [ 52 ]. We found pgaC in the genome sequence of the P. shigelloides strain used in this experiment, but did not find pgaA and pgaB .…”

Section: Discussionmentioning

confidence: 99%

The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides

Yan

Guo

et al. 2021

BMC Microbiol

View full text Add to dashboard Cite

Background The anoxic redox control binary system plays an important role in the response to oxygen as a signal in the environment. In particular, phosphorylated ArcA, as a global transcription factor, binds to the promoter regions of its target genes to regulate the expression of aerobic and anaerobic metabolism genes. However, the function of ArcA in Plesiomonas shigelloides is unknown. Results In the present study, P. shigelloides was used as the research object. The differences in growth, motility, biofilm formation, and virulence between the WT strain and the ΔarcA isogenic deletion mutant strain were compared. The data showed that the absence of arcA not only caused growth retardation of P. shigelloides in the log phase, but also greatly reduced the glucose utilization in M9 medium before the stationary phase. The motility of the ΔarcA mutant strain was either greatly reduced when grown in swim agar, or basically lost when grown in swarm agar. The electrophoretic mobility shift assay results showed that ArcA bound to the promoter regions of the flaK, rpoN, and cheV genes, indicating that ArcA directly regulates the expression of these three motility-related genes in P. shigelloides. Meanwhile, the ability of the ΔarcA strain to infect Caco-2 cells was reduced by 40%; on the contrary, its biofilm formation was enhanced. Furthermore, the complementation of the WT arcA gene from pBAD33-arcA+ was constructed and all of the above features of the pBAD33-arcA+ complemented strain were restored to the WT level. Conclusions We showed the effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides, and demonstrated that ArcA functions as a positive regulator controls the motility of P. shigelloides by directly regulating the expression of flaK, rpoN and cheV genes.

show abstract

Putative virulence factors of Plesiomonas shigelloides

Cited by 15 publications

References 77 publications

Plesiomonas shigelloides, a potential pathogen of enteritis in Ictalurus punctatus

Plesiomonas shigelloides, a potential pathogen of enteritis in Ictalurus punctatus

Helicobacter pylori Virulence Factors Exploiting Gastric Colonization and its Pathogenicity

The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides

Contact Info

Product

Resources

About