Putrescine Effect on Nitrate Reductase Activity, Organic Nitrogen, Protein, and Growth in Heavy Metal and Salinity Stressed Mustard Seedlings

Singh, Devender; Varma, S. K.; Mishra, Saurabh

doi:10.1023/a:1022360612976

Cited by 23 publications

(5 citation statements)

References 18 publications

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“…We found that VvNR1 expression increased with the NO 3 − ratio, whereas NR activity and gene expression were not significantly correlated. However, this may be a result of the additional environmental factors that regulate NR activity beyond the presence of NO 3 ‐ 36, 37 …”

Section: Discussionmentioning

confidence: 99%

“…However, this may be a result of the additional environmental factors that regulate NR activity beyond the presence of NO 3 -. 36,37 The correlation between sucrose and nitrogen metabolism in berries and leaves Competition occurs when sucrose and nitrogen metabolism have a common need that cannot be met, and excess nitrogen does not alleviate this competition. Therefore, the balance between the nitrogen and carbon metabolic pathways is widely recognized as being of paramount importance toward improving nitrogen use efficiency.…”

Section: Nomentioning

confidence: 99%

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Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Yin

Wang

et al. 2020

J Sci Food Agric

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BACKGROUND: The quality of wine is highly dependent on the quality of berries. Development of berries is influenced by the type and ratio of different nitrogen supplies in the soil. To understand the impact of varying sources and levels of nitrate and ammonium on sucrose and nitrogen metabolism of Vitis vinifera cv. Cabernet Sauvignon, we tested nutrient solutions with four NO 3 −-N:NH 4 +-N ratios (100:0, 75:25, 50:50, 0:100) through the root system. RESULTS: The form and quantity of nitrogen affected berries and leaves with source-sink relationships. Soluble sugar levels were significantly higher in plants treated with mixed nitrogen sources (75:25 and 50:50) compared to single nitrogen sources (100:0 and 0:100). In particular, treating plants with mixed nitrogen source at a 75:25 ratio resulted in 22% higher fructose levels in berries compared to the 50:50 treatment. In addition, mixed nitrogen treatments resulted in significantly higher amino acid levels and protein content. Mixed nitrogen substrates also increased the expression of enzymes involved in both nitrogen and sucrose metabolism. CONCLUSION: Plants did not maximize the nitrogen supply when single form nitrogen was provided, and the mixed nitrogen substrates consistently increased the amount of available carbon and nitrogen in the berries and leaves. We found that NO 3 − N: NH 4 +-N ratio of 75:25 was the optimum formula for improving nitrogen and sucrose metabolism, and reducing the competition between nitrogen and sucrose. By examining the nutrient utilization of plants cultivated with different nitrogen forms, the present study provides insights into improving cultivation and production practices.

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Section: Discussionmentioning

confidence: 99%

Section: Nomentioning

confidence: 99%

Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Yin

Wang

et al. 2020

J Sci Food Agric

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“…In contrast, polyamines improved N 2 , K + , Ca 2+ and Mg 2+ uptake and translocation (Table 4) through improving root growth and development and adjusting water potential in plant cells (Gill and Tuteja, 2010). Moreover, enhanced N 2 content in mung bean leaves may be attributed to rising nitrate reductase activity by polyamines (Singh et al, 2002). The decreased grain yield of mung bean plants under water stress (Table 4) is associated with negative effects of water stress on physiological and biochemical activities under such conditions (Figures 1-7 and tables 3 and 4).…”

Section: Application Of Polyamines By Reducing Polyaminementioning

confidence: 97%

Monitoring cell energy, physiological functions and grain yield in field-grown mung bean exposed to exogenously applied polyamines under drought stress

Ghassemi

Farhangi‐Abriz

Faegi-Analou

et al. 2018

J. Soil Sci. Plant Nutr.

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Polyamines are new plant growth regulators and intracellular messengers that regulate plant growth, development, and responses to abiotic environmental stresses. However, little is known about the impact of different polyamines on physiological and biochemical processes of field-grown plants exposed to water deficit stress. A two-year field experiment was conducted as a split-plot based on a randomized complete block design with four replications. Water treatments (Irrigation after 70 and 170 mm evaporation from class A evaporation pan) were assigned to main plots, and foliar application of polyamines including Putrescine, Spermidine and Spermine (at 0 and 0.1 mM) was apportioned to subplots. Average of two years results showed that compared with control, water stress significantly (P < 0.05) reduced adenosine three phosphate (ATP), chlorophyll content index (CCI) and chlorophyll stability index (CSI), rubisco activity, efficiency of photosystem II (Fv/Fm), endogenous polyamine contents, membrane stability index (MSI), nitrogen (N 2 ), potassium (K + ), calcium (Ca 2+ ), magnesium (Mg 2+ ), and grain yield, while polyamine oxidase activity, antioxidant enzymes activity, reactive oxygen species (ROS) and lipid peroxidation (MDA) increased significantly (P < 0.05) upon exposure to water deficit stress. However, exogenous application of polyamines improved ATP content, CCI, CSI, rubisco activity, Fv/Fm, endogenous polyamine content, MSI, antioxidant enzymes activity, N 2 , K + , Ca 2+ , Mg 2+ , and grain yield, while decreased ROS level and MDA content. Compared with the other polyamines, Putrescine showed better effects on physiological, biochemical and agronomical performance in mung bean plants under both irrigation treatments.

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“…Increased amount of nitrate in the cell induces the gene encoding nitrate reductase transcription, resulting in increased activity of the enzyme (23). One of the main causes of reduced nitrate reductase activity is the restricted availability of nitrate and nitrogen from heavy metal toxicity (13,21,24). Treatment with Ni clearly demonstrate the toxic effect on nitrogen assimilation.…”

Section: Resultsmentioning

confidence: 99%

Influence of Cd and Ni on Content of N, P, K, Nitrates and Activity of Nitrate Reductase in Clones ofSalix Spp.

Borišev

Pajević

Nikolić

et al. 2010

Biotechnology & Biotechnological Equipment

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Putrescine Effect on Nitrate Reductase Activity, Organic Nitrogen, Protein, and Growth in Heavy Metal and Salinity Stressed Mustard Seedlings

Cited by 23 publications

References 18 publications

Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Monitoring cell energy, physiological functions and grain yield in field-grown mung bean exposed to exogenously applied polyamines under drought stress

Influence of Cd and Ni on Content of N, P, K, Nitrates and Activity of Nitrate Reductase in Clones ofSalix Spp.

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