Putrescine modulation of acute activation of the β-adrenergic system in the left atrium of rat

Bordallo, Carmen; Cantabrana, Begoña; Velasco, Lucía; Secades, Lorena; Meana, Clara; Méndez, Míriam; Bordallo, Javier; Sánchez, Manuel

doi:10.1016/j.ejphar.2008.07.069

Cited by 12 publications

(11 citation statements)

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“…Furthermore, tyramine, a TA, produced an indirect sympathetic effect [53,54] and polyamines bind and activate β 1 -adrenoceptors in rat hearts [55], β 2 -adrenoceptors in the bovine trachea [19], and human stably transfected β 1 - and β 2 -adrenoceptors in CHO cells [56]. However, in intestinal rings neither spermine nor isoamylamine seemed to activate β-adrenoceptors, since propranolol did not modify their inhibitory effects on motility.…”

Section: Discussionmentioning

confidence: 99%

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Sánchez

Suárez

Andrés

et al. 2017

Food & Nutrition Research

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Background: Gastrointestinal motility modulatory factors include substances of the intestinal content, such as polyamines and trace amines (TAs), the focus of this study.Methods: The amines of food, intestinal content and from faecal bacteria of Swiss mice were determined by HPLC and functionally characterised in isolated distal ileum and medial colon rings.Results: Mouse food and intestinal content contain polyamines (spermidine>putrescine>spermine) and TAs (isoamylamine>cadaverine). Intestinal bacteria mainly produce putrescine and cadaverine. The amines inhibited the spontaneous motility of the ileum (0.1-3 mM) and colon rings (0.01-3 mM, with lower IC50), with: spermine~isoamylamine~spermidine. Spermine inhibition was tetrodotoxin (TTX)-insensitive, while isoamylamine was TTX-sensitive, suggesting neural control. Mainly in the ileum, isoamylamine (3 mM) elicited acute effects modified by TTX, atropine and propranolol, and suppressed by spermine (3 mM), not being localized at the smooth muscle level. The amines assayed (3 mM), except putrescine and cadaverine in the ileum and isoamylamine in the colon, antagonised acetylcholine (ACh, 0.1 mM)-elicited phasic contractions. Isoamylamine and spermine in colon relaxed KCl (100 mM)-elicited tonic contractions, suggesting an effect on smooth muscle, but did not justify the suppression of motility caused by spermine and isoamylamine.Conclusions: Polyamines and TAs of the intestinal content might act on chemosensors and modulate intestinal peristalsis.

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Section: Discussionmentioning

confidence: 99%

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Sánchez

Suárez

Andrés

et al. 2017

Food & Nutrition Research

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“…Because ODC and polyamines are required for cell growth, 37 the common belief is that the induction of cardiac ODC could be an obligatory step in heart hypertrophy induced by catecholamines. 38 Nevertheless, experiments using transgenic mice overexpressing ODC in the heart demonstrated that the robust expression of ODC is not sufficient by itself to promote heart hypertrophy but indicated that ODC may cooperate with other factors inducing cardiac growth. 39 Cardiac ODC induction by b-adrenoreceptor agonist was associated with the phosphorylation of ERKs, and the blockade of ODC induction by L-type calcium channel antagonists also prevented the phosphorylation of ERKs.…”

Section: Discussionmentioning

confidence: 99%

Mitogen‐Activated Protein Kinase and Intracellular Polyamine Signaling Is Involved in TRPV1 Activation–Induced Cardiac Hypertrophy

Chen

Xin

Liu

et al. 2016

JAHA

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BackgroundThe transient receptor potential vanilloid type 1 (TRPV1) is expressed in the cardiovascular system, and increased TRPV1 expression has been associated with cardiac hypertrophy. Nevertheless, the role of TRPV1 in the pathogenesis of cardiac hypertrophy and the underlying molecular mechanisms remain unclear.Methods and ResultsIn cultured cardiomyocytes, activation of TRPV1 increased cell size and elevated expression of atrial natriuretic peptide mRNA and intracellular calcium level, which was reversed by TRPV1 antagonist capsazepine. Increased expression of phosphorylated calmodulin‐dependent protein kinase IIδ and mitogen‐activated protein kinases were found in TRPV1 agonist capsaicin‐treated cardiomyocytes. Selective inhibitor of calmodulin‐dependent protein kinase IIδ decreased phosphorylation of extracellular signal–regulated kinases and p38. Capsaicin induced an increase in expression of ornithine decarboxylase protein, which is the key enzyme in polyamine biosynthesis in cardiomyocytes. Nevertheless, there was no obvious change of ornithine decarboxylase expression in TRPV1 knockdown cells after capsaicin treatment, and specific inhibitors of calmodulin‐dependent protein kinase IIδ or p38 downregulated the capsaicin‐induced expression of ornithine decarboxylase. Capsazepine alleviated the increase in cross‐sectional area of cardiomyocytes and the ratio of heart weight to body weight and improved cardiac function, including left ventricular internal end‐diastolic and ‐systolic dimensions and ejection fraction and fractional shortening percentages, in mice treated with transverse aorta constriction. Capsazepine also reduced expression of ornithine decarboxylase and cardiac polyamine levels. Transverse aorta constriction induced increases in phosphorylated calmodulin‐dependent protein kinase IIδ and extracellular signal–regulated kinases, and p38 and Serca2a were attenuated by capsazepine treatment.ConclusionsThis study revealed that the mitogen‐activated protein kinase signaling pathway and intracellular polyamines are essential for TRPV1 activation–induced cardiac hypertrophy.

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“…However, experiments using transgenic mice overexpressing ODC in the heart demonstrated that the robust expression of ODC is not sufficient by itself to promote heart hypertrophy but indicated that ODC may cooperate with factors inducing cardiac growth [Shantz et al, 2001]. In this regard, it has been suggested that putrescine may function as a low affinity agonist on b-adrenoreceptors and modulate acute responses mediated by b-adrenoreceptors [Bordallo et al, 2008]. Our results suggest that the stimulation of ODC by the beta adrenergic agonists is a characteristic of normal heart mice, which is markedly diminished once that the heart becomes hypertrophic.…”

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confidence: 99%

The induction of cardiac ornithine decarboxylase by β₂‐adrenergic agents is associated with calcium channels and phosphorylation of ERK1/2

López-Contreras

Morena

Ramos‐Molina

et al. 2013

J of Cellular Biochemistry

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The role that the induction of cardiac ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, by beta-adrenergic agents may have in heart hypertrophy is a controversial issue. Besides, the signaling pathways related to cardiac ODC regulation have not been fully elucidated. Here we show that in Balb C mice the stimulation of cardiac ODC activity by adrenergic agents was mainly mediated by β2 -adrenergic receptors, and that this induction was lower in the hypertrophic heart. Interestingly, this stimulation was abolished by the L-calcium channel antagonists verapamil and nifedipine. In addition, whereas the treatment with β2 -adrenergic agents was associated to both the increases in ODC, ODC-antizyme inhibitor 1 (AZIN1), c-fos and c-myc mRNA levels and the phosphorylation of CREB and MAP kinases ERK1 and ERK2 (ERK1/2), the co-treatment with L-calcium channel blockers differentially prevented most of these changes. These results suggest that the stimulation of cardiac ODC by β2 -adrenergic agents is associated with the activation of MAP kinases through the participation of L-calcium channels, and that by itself p-CREB does not appear to be sufficient for the transcriptional activation of ODC. In addition, post-translational mechanisms related with the induction of AZIN1 appear to be related to the increase of cardiac ODC activity.

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Putrescine modulation of acute activation of the β-adrenergic system in the left atrium of rat

Cited by 12 publications

References 29 publications

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Mitogen‐Activated Protein Kinase and Intracellular Polyamine Signaling Is Involved in TRPV1 Activation–Induced Cardiac Hypertrophy

The induction of cardiac ornithine decarboxylase by β₂‐adrenergic agents is associated with calcium channels and phosphorylation of ERK1/2

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Putrescine modulation of acute activation of the β-adrenergic system in the left atrium of rat

Cited by 12 publications

References 29 publications

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Mitogen‐Activated Protein Kinase and Intracellular Polyamine Signaling Is Involved in TRPV1 Activation–Induced Cardiac Hypertrophy

The induction of cardiac ornithine decarboxylase by β2‐adrenergic agents is associated with calcium channels and phosphorylation of ERK1/2

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The induction of cardiac ornithine decarboxylase by β₂‐adrenergic agents is associated with calcium channels and phosphorylation of ERK1/2