Putrescine oxidase/peroxidase-co-immobilized and mediator-less mesoporous microelectrode for diffusion-controlled steady-state amperometric detection of putrescine

Xia, Hong-qi; Kitazumi, Yuki; Shirai, Osamu; Ohta, Hirokazu; Kurihara, Shin; Kano, Kenji

doi:10.1016/j.jelechem.2017.09.056

Cited by 22 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…corresponding to the DET-type bioelectrocatalysis for the reduction of H2O2 with POD, as has been reported previously. 51,52 The limiting current densities were 0.29 ± 0.05 and 0.28 ± 0.04 mA cm -2 at the electrodes anodized in the oxalic acid and glucose solutions, respectively. POD modified on a planar gold electrode did not produce a catalytic H2O2-reduction wave (Fig.…”

Section: Det-type Bioelectrocatalysis At Bod-and Pod-adsorbed Electrodesmentioning

confidence: 96%

“…66 The catalytic wave observed in this work is very similar to definitive DET-type catalytic waves of POD. 51,52 This means that porous gold electrodes are superior scaffolds for the DET-type reaction of POD in terms of simplicity of the fabrication in constructing a versatile biosensing system. The porous electrode anodized in the oxalic acid solution exhibited better performance than that anodized in the glucose solution with respect to the limiting current and the half-wave potential.…”

Section: Det-type Bioelectrocatalysis At Bod-and Pod-adsorbed Electrodesmentioning

confidence: 99%

“…[46][47][48][49][50] However, it has recently been reported that POD can work as a DET-type biocatalyst at suitable mesoporous electrodes to reduce H2O2 at a potential close to the redox potential of the protoheme (Compound I) of POD (0.7 V vs. Ag/AgCl at pH 7). [51][52][53] Such DET-type reactions of POD will draw attention of constructing bienzyme-type biosensors without any mediator. For practical purposes, it is important to easily construct scaffolds for DETtype enzymes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

et al. 2018

Self Cite

View full text Add to dashboard Cite

In this study, nanostructured porous gold electrodes were prepared by the anodization of gold in the presence of oxalic acid or glucose as a reductant, and applied as scaffolds for direct electron transfer (DET)-type bioelectrocatalysis. Gold cations generated in the anodization seem to be reduced by the reductant to construct a porous gold structure. The DET-type performance of the electrode was examined using two DET-type model enzymes, bilirubin oxidase (BOD) and peroxidase (POD), for the four-electron reduction of dioxygen and the two-electron reduction of peroxide, respectively. BOD and POD on the anodized porous gold electrodes exhibited well-defined sigmoidal steady-state waves corresponding to DET-type bioelectrocatalysis. Scanning electron microscopy images revealed sponge-like pores on the electrodes. The anodized porous gold electrodes demonstrate promise as scaffolds for DET-type bioelectrocatalysis.

show abstract

Section: Det-type Bioelectrocatalysis At Bod-and Pod-adsorbed Electrodesmentioning

confidence: 96%

Section: Det-type Bioelectrocatalysis At Bod-and Pod-adsorbed Electrodesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…83 DET-type steady-state diffusion-controlled electrolysis was realized for H 2 O 2 reduction at HRP-adsorbed mesoporous microelectrodes. 18,52 The electrocatalytic system can be coupled with a variety of oxidases such as glucose oxidase (GOD), 18 putrescine oxidase, 84 and pyruvate oxidase. 85 Kinetic analysis on the series resistance model can be used to optimize the performance of such bienzyme-type biosensors.…”

Section: Biosensorsmentioning

confidence: 99%

Fundamentals and Applications of Redox Enzyme-functionalized Electrode Reactions

Kano

2019

Electrochemistry

Self Cite

View full text Add to dashboard Cite

Electrochemical coupling of redox enzyme reactions, called bioelectrocatalysis, has been attracting great attention over the last four decades. It has become an important technology that can be applied to a wide range of bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This article presents an overview of the basic concepts of steady-state catalytic waves of mediated-and direct electron transfer (DET)-type bioelectrocatalysis. Several equations that can be used for the analysis of steady-state waves are introduced. The analysis may provide important thermodynamic and kinetic parameters that can be used not only for performance evaluation of the devices but also for fundamental research on the enzymes. Important progress made on how to tune electrode surfaces and enzymes for DET-type reactions are presented. Applications to bioelectrochemical devices are also summarized with emphasis on the achievements recorded in our research group.

show abstract

“…If one can use biosensors without calibration, it is easy to monitor the concentration of target analytes. In this sense, microelectrode detection was introduced to develop amperometric biosensors [ 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Nonlinear diffusion of the substrate at microelectrodes causes diffusion-controlled steady-state currents.…”

Section: Introductionmentioning

confidence: 99%

Development Perspective of Bioelectrocatalysis-Based Biosensors

Adachi

Kitazumi

Shirai

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

show abstract

Putrescine oxidase/peroxidase-co-immobilized and mediator-less mesoporous microelectrode for diffusion-controlled steady-state amperometric detection of putrescine

Cited by 22 publications

References 34 publications

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

Fundamentals and Applications of Redox Enzyme-functionalized Electrode Reactions

Development Perspective of Bioelectrocatalysis-Based Biosensors

Contact Info

Product

Resources

About