Puumala Hantavirus Viremia Diagnosed by Real-Time Reverse Transcriptase PCR Using Samples from Patients with Hemorrhagic Fever and Renal Syndrome

Evander, Magnus; Eriksson, Iréne; Pettersson, Lisa; Juto, Per; Ahlm, Clas; Olsson, Gert E.; Bucht, Gösta; Allard, Annika

doi:10.1128/jcm.01902-06

Cited by 90 publications

(93 citation statements)

References 36 publications

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“…For NE diagnosis, we used an immunofl uorescence assay to detect PUUV-reactive immunoglobulin (Ig) M and IgG antibodies in serum of all patients with clinically suspected NE (6). A real-time reverse transcription-PCR (6) was used to obtain an amplifi cation product from 1 patient sample.…”

Section: The Studymentioning

confidence: 99%

Outbreak of Puumala Virus Infection, Sweden

Pettersson

Boman

Juto

et al. 2008

Emerg. Infect. Dis.

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Section: The Studymentioning

confidence: 99%

Outbreak of Puumala Virus Infection, Sweden

Pettersson

Boman

Juto

et al. 2008

Emerg. Infect. Dis.

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“…TaqMan™-based, real-time reverse transcriptionpolymerase chain reaction (RT-PCR) can rapidly detect and identify the selected gene targets found within the genomes of these RNA viruses. Although many research groups have developed and tested standard and real-time RT-PCR assays for detection of various species and corresponding strains of Ebola, [3][4][5][6][7][8] Marburg, 4,8,9 Lassa 8, 10-12 Junin, 13 and hantavirus, [14][15][16][17][18] a comprehensive panel of TaqMan™-based real-time PCR assays using identical cycling conditions is not currently available.…”

mentioning

confidence: 99%

Comprehensive Panel of Real-Time TaqMan™ Polymerase Chain Reaction Assays for Detection and Absolute Quantification of Filoviruses, Arenaviruses, and New World Hantaviruses

Trombley¹,

Wachter²,

Garrison³

et al. 2010

The American Society of Tropical Medicine and Hygiene

199

217

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Viral hemorrhagic fever is caused by a diverse group of single-stranded, negative-sense or positive-sense RNA viruses belonging to the families Filoviridae (Ebola and Marburg), Arenaviridae (Lassa, Junin, Machupo, Sabia, and Guanarito), and Bunyaviridae (hantavirus). Disease characteristics in these families mark each with the potential to be used as a biological threat agent. Because other diseases have similar clinical symptoms, specific laboratory diagnostic tests are necessary to provide the differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. We designed 48 TaqMan™-based polymerase chain reaction (PCR) assays for specific and absolute quantitative detection of multiple hemorrhagic fever viruses. Forty-six assays were determined to be virus-specific, and two were designated as pan assays for Marburg virus. The limit of detection for the assays ranged from 10 to 0.001 plaque-forming units (PFU)/PCR. Although these real-time hemorrhagic fever virus assays are qualitative (presence of target), they are also quantitative (measure a single DNA/RNA target sequence in an unknown sample and express the final results as an absolute value (e.g., viral load, PFUs, or copies/mL) on the basis of concentration of standard samples and can be used in viral load, vaccine, and antiviral drug studies.

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“…Semptomların başladığı andan itibaren serum, idrar, doku biyopsi materyali gibi klinik örneklerde RT-PCR ile hantavirus saptanabilir. Semptomların başlangıcında henüz serumda IgM negatifken, hatta henüz semptomlar başlamadan önce, RT-PCR ile serumda hantavirus RNA'sı saptanabilir (23). Hantavirus tiplerinden ANDV ile infekte indeks bir vakanın aile bireylerinde periyodik olarak RT-PCR ile hantavirus RNA'sı araştırılmış ve bu kişilerde semptomlar başlamadan 5-15 gün önce serumda PCR'ın pozitifleştiği saptanmıştır (2,22).…”

Section: Tanıunclassified

“…Ancak son yıllarda yapılan bazı yayınlarda hastalığın ikinci-üçüncü haftasında bile hasta serumda PUUV RNA'sı saptandığı bildirilmektedir. İsveç'te RT-PCR ile yapılan bir çalışmada RSKA olgularında serumda PUUV-RNA hastalığın 3-9. günleri arasında negatifleşmiştir (23). Rusya'da yapılan bir çalışmada 12 RSKA olgusunda hastalığın 4. gününden itibaren serumda periyodik olarak PUUV RNA'sı ölçülmüş ve hastaların başlangıç PUUV-RNA düzeyi 4x10 4 -1x10 6 kopya/ml olarak saptanmıştır (24).…”

Section: Tanıunclassified

Hantavirus Infections

Çelebi¹

2012

Klimik Dergisi

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ÖzetHantaviruslar, Bunyaviridae ailesinin Hantavirus cinsinde yer alan, kemirici (rodent) veya böcekçil (insektivor) kaynaklı, zarflı bir RNA virusu grubudur. Her hantavirus tipi o tipe özgü bir kemirici türü tarafından taşınır. Günümüze kadar 45 civarında hantavirus tipi saptanmış ve en az 20 tanesi insanlarda infeksiyon etkeni olarak gösterilmiştir. Hantaviruslar kemiricilerde çoğunlukla asemptomatik fakat kronik bir infeksiyona neden olur. Kemiriciler idrar ve dışkılarıyla aylarca çevreye virus saçar-lar. Amerika kıtasında bulunan hantavirus tipleri (Sin Nombre virusu, Andes virusu, Laguna Negra virusu, New York virusu vb.) hantavirus pulmoner sendromuna (HPS) neden olur ve oluşan infeksiyonun fatalitesi oldukça yüksektir (%30 civarında AbstractHantaviruses belonging to the Bunyaviridae family, and the genus Hantavirus, are a group of rodent-or insectivore-borne RNA viruses. Each Hantavirus type is carried by a specific rodent host species. Up to now, approximately 45 hantavirus types have been isolated and at least 20 have been identified as infectious agents in humans. Hantaviruses mostly cause asymptomatic but chronic infections in rodents. Rodents spread the viruses on the surfaces in the environment with their urine and feces during several months. Hantavirus types which exist in the Americas (Sin Nombre virus, Andes virus, Laguna Negra virus, New York virus etc.) cause Hantavirus pulmonary syndrome (HPS) and the fatality rate of the disease is quite high (around 30%). Hantavirus types which exist in Europe and Asia (Hantaan virus [HTNV], Seoul virus [SEOV], Dobrova virus [DOBV], Puumala virus [PUUV]) cause hemorrhagic fever with renal syndrome (HFRS). HTNV and SEOV in Asia, and PUUV and DOBV in Europe are the major agents of HFRS. Infection caused by HTNV or DOBV is a more serious disease with a fatality rate around 5-10%, while infection caused by PUUV is a mild disease with a fatality rate around 0.1%. Ribavirin was found to be effective in the treatment of HFRS in a few studies conducted in China and Korea. However, it was found to be ineffective in the treatment of HPS in America. The most effective method for prevention seems to be vaccination and studies are still ongoing for a safe and effective vaccine. The first human hantavirus outbreak in Turkey confirmed clinically and serologically was detected in the Zonguldak-Bartın region in February 2009. PUUV and DOBV were isolated from tissue samples of rodents captured in the outbreak region by using polymerase chain reaction and viral cell culture. Considering the current rodent and insectivore species in the country, in addition to PUUV and DOBV, the presence of Saaremaa virus, SEOV, Tula virus and Seewis virus may be anticipated in Turkey. Klimik Dergisi 2011; 24(3): 139-49.

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Puumala Hantavirus Viremia Diagnosed by Real-Time Reverse Transcriptase PCR Using Samples from Patients with Hemorrhagic Fever and Renal Syndrome

Abstract: Puumala virus (PUUV) is the endemic hantavirus in northern Sweden and causes nephropathia epidemica (NE),

Cited by 90 publications

References 36 publications

Outbreak of Puumala Virus Infection, Sweden

Outbreak of Puumala Virus Infection, Sweden

Comprehensive Panel of Real-Time TaqMan™ Polymerase Chain Reaction Assays for Detection and Absolute Quantification of Filoviruses, Arenaviruses, and New World Hantaviruses

Hantavirus Infections

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