PUX10 Is a CDC48A Adaptor Protein That Regulates the Extraction of Ubiquitinated Oleosins from Seed Lipid Droplets in Arabidopsis

Deruyffelaere, Carine; Purkrtová, Zita; Bouchez, Isabelle; Collet, Boris; Cacas, Jean‐Luc; Chardot, Thierry; Gallois, Jean‐Luc; d’Andréa, Sabine

doi:10.1105/tpc.18.00275

Cited by 75 publications

(90 citation statements)

References 86 publications

(125 reference statements)

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“…These distinct motifs designate oleosins toward different degradation pathways according to the ubiquitination type. Deruyffelaere et al further confirmed that PUX10 localized to OBs interacts directly with ubiquitinated oleosins and mediates dislocation of oleosins by the AAA ATPase CDC48A (Deruyffelaere et al, 2018;Kretzschmar et al, 2018).…”

Section: Interactions Between Ob-associated Proteins and Other Proteinsmentioning

confidence: 87%

“…PUX10 localizes to OBs and binds to the ubiquitinated oleosins via its hydrophobic domain and interacts with ubiquitin and CDC48A via its UBA and UBX domains, respectively. As an adaptor, PUX10 recruits CDC48A to ubiquitinated oleosins, leading to dislocation of oleosins from OBs via the segregase activity of CDC48A (Deruyffelaere et al, 2018;Kretzschmar et al, 2018).…”

Section: Oleosins Play An Important Role In Ob Formation and Degradationmentioning

confidence: 99%

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New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

Shao

Liu

et al. 2019

Front. Plant Sci.

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Oil bodies (OBs) are ubiquitous dynamic organelles found in plant seeds. They have attracted increasing attention recently because of their important roles in plant physiology. First, the neutral lipids stored within these organelles serve as an initial, essential source of energy and carbon for seed germination and post-germinative growth of the seedlings. Secondly, they are involved in many other cellular processes such as stress responses, lipid metabolism, organ development, and hormone signaling. The biological functions of seed OBs are dependent on structural proteins, principally oleosins, caleosins, and steroleosins, which are embedded in the OB phospholipid monolayer. Oleosin and caleosin proteins are specific to plants and mainly act as OB structural proteins and are important for the biogenesis, stability, and dynamics of the organelle; whereas steroleosin proteins are also present in mammals and play an important role in steroid hormone metabolism and signaling. Significant progress using new genetic, biochemical, and imaging technologies has uncovered the roles of these proteins. Here, we review recent work on the structural or metabolic roles of these proteins in OB biogenesis, stabilization and degradation, lipid homeostasis and mobilization, hormone signal transduction, stress defenses, and various aspects of plant growth and development.

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Section: Interactions Between Ob-associated Proteins and Other Proteinsmentioning

confidence: 87%

Section: Oleosins Play An Important Role In Ob Formation and Degradationmentioning

confidence: 99%

New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

Shao

Liu

et al. 2019

Front. Plant Sci.

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“…It seems possible, therefore, that the slight enlargement of LBs during bud development is based on the continuation of TAG biosynthesis at free cytosolic LBs [28]. In mature buds, some LBs might show coalescence (Figure 1e,g), which could be caused either by LB expansion and the resulting changes in LB surface tension [4,10], or by gradual degradation of OLEOSINs [73,74].…”

Section: Discussionmentioning

confidence: 99%

Plant Lipid Bodies Traffic on Actin to Plasmodesmata Motorized by Myosin XIs

Veerabagu

Paul

Rinne

et al. 2020

IJMS

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Late 19th-century cytologists observed tiny oil drops in shoot parenchyma and seeds, but it was discovered only in 1972 that they were bound by a half unit-membrane. Later, it was found that lipid bodies (LBs) arise from the endoplasmic reticulum. Seeds are known to be packed with static LBs, coated with the LB-specific protein OLEOSIN. As shown here, apices of Populus tremula x P. tremuloides also express OLEOSIN genes and produce potentially mobile LBs. In developing buds, PtOLEOSIN (PtOLE) genes were upregulated, especially PtOLE6, concomitant with LB accumulation. To investigate LB mobility and destinations, we transformed Arabidopsis with PtOLE6-eGFP. We found that PtOLE6-eGFP fusion protein co-localized with Nile Red-stained LBs in all cell types. Moreover, PtOLE6-eGFP-tagged LBs targeted plasmodesmata, identified by the callose marker aniline blue. Pharmacological experiments with brefeldin, cytochalasin D, and oryzalin showed that LB-trafficking requires F-actin, implying involvement of myosin motors. In a triple myosin-XI knockout (xi-k/1/2), transformed with PtOLE6-eGFP, trafficking of PtOLE6-eGFP-tagged LBs was severely impaired, confirming that they move on F-actin, motorized by myosin XIs. The data reveal that LBs and OLEOSINs both function in proliferating apices and buds, and that directional trafficking of LBs to plasmodesmata requires the actomyosin system.

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“…1). The most abundant LD protein, oleosin, is embedded in the LD membrane and helps maintain LD structure and regulates lipid mobilization via its extraction and turnover by the ubiquitin-proteasome system (Pyc et al, 2017;Deruyffelaere et al, 2018).…”

Section: Discovering Lipid Droplet Proteins: From Seeds To Seedlingsmentioning

confidence: 99%

“…It has been suggested that LD proteins act to shield TAGs from lipolytic enzymes; when removed, accessible lipids are extracted and mobilized. Alternatively, LD proteins may interact with cytoplasmic lipases and other regulatory proteins to stimulate lipolysis (Deruyffelaere et al, 2018).…”

Section: Discovering Lipid Droplet Proteins: From Seeds To Seedlingsmentioning

confidence: 99%

Discovering Lipid Droplet Proteins: From Seeds to Seedlings

Richardson

2020

Plant Physiol.

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PUX10 Is a CDC48A Adaptor Protein That Regulates the Extraction of Ubiquitinated Oleosins from Seed Lipid Droplets in Arabidopsis

Cited by 75 publications

References 86 publications

New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

Plant Lipid Bodies Traffic on Actin to Plasmodesmata Motorized by Myosin XIs

Discovering Lipid Droplet Proteins: From Seeds to Seedlings

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