PVA/anionic collagen membranes as drug carriers of ciprofloxacin hydrochloride with sustained antibacterial activity and potential use in the treatment of ulcerative keratitis

Abstract

Devices such as contact lenses and collagen shields have been used to improve the antibiotic bioavailability of eye drops formulations in the treatment of ulcerative keratitis. Nevertheless, these devices are not sustained drug delivery systems, and a combination with eye drops is necessary. In animal patients, it requires constant supervision by trained personnel to avoid device loss, which increases the cost of treatment. In this study, PVA/anionic collagen membranes containing ciprofloxacin or tobramycin were prepared using two different methodologies, and the release, physical and antimicrobial properties were evaluated. The membrane containing ciprofloxacin was selected as a sustained drug delivery system with antibacterial activity against Staphylococcus aureus and Escherichia coli during 48 h. Despite to be opaque, due to its heterogeneous morphology, this membrane had the adequate mechanical strength, water content, hydrophilicity, water vapor permeability, and surface pH to interact with cornea without causing discomfort. In the surface of this membrane it was observed dispersed collagen fibrils which could serve as a substrate for corneal proteinases, contributing to the reduction in stromal damage and enhancing the epithelium regeneration. These results encourage the idea these membranes are new cost-effective and safe alternatives to treat corneal ulcers in animal patients.

Polymer-based dual drug delivery system for targeted treatment of fluoroquinolone-resistant Staphylococcus aureus mediated infections

“…When CIP was loaded in the bi-functional PEG glyoxylic aldehyde -Chitosan cross-linked hydrogels at 3%, 6%, 9%, and 12% w/v polymer concentration, wherein the high polymer (9% and 12%) hydrogels demonstrated the sustained release of the drug for up to 24 h (≥ 80%) and the low polymer (3% and 6%) hydrogels had 85-95% release within 10-12 h 39 . In a study involving PVA/ anionic collagen membranes loaded with CIP, sustained release of the drug was observed reaching 95% at the end of 48 h, 40 which was considered better than the plain PVA or PVA-gelatin inserts 41,42 .…”

In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis

“…Antimicrobial-resistant strains of various types of bacteria derived from ocular infection have been isolated, including S. aureus, coagulase-negative staphylococci, P. aeruginosa, Corynebacterium spp., and Escherichia coli [25][26][27][28][29]. In recent years, novel alternative or additional therapeutic agents to antibiotics have been developed for ocular infections caused by antimicrobial-resistant bacteria, including antiseptic hexamidine diisethionate ophthalmic solution [30] and devices to improve the antibiotic bioavailability of eye drops such as polyvinyl alcohol/anionic collagen membranes [31] and ofloxacin-loaded polymeric nanoparticles [32]. We have previously reported the effectiveness of phage eye drops for the mouse model of P. aeruginosa keratitis [12] and others have reported a case with vancomycin-resistant S. aureus keratitis successfully treated by a bacteriophage eyedrop [33].…”

Physiochemical, antioxidant, and food simulant release properties of collagen‐carboxymethyl cellulose films enriched with Berberis lyceum root extract for biodegradable active food packaging

“…Researchers have been reported that around ~45% of collagen can be isolated from fishbone and ~50% from fish skin (Ahmed et al, 2020; Nagai & Suzuki, 2000). Fish collagen serves as the best alternative to land‐based collagen and is used to prepare collagen‐based films for food packaging and drug delivery (Daza et al, 2020; Jiang et al, 2020).…”