PVA-Based Electrospun Biomembranes with Hydrolyzed Collagen and Ethanolic Extract of Hypericum perforatum for Potential Use as Wound Dressing: Fabrication and Characterization

Abstract

Biological, physicochemical, structural, and thermal properties of PVA-based electrospun wound dressings added with hydrolyzed collagen (HC) and different concentrations of Hypericum perforatum ethanolic extract (EEHP) were studied. Membrane characterization was carried out by X-ray diffraction, Fourier infrared spectroscopy, differential scanning calorimetry, barrier properties, scanning electron microscopy, image analysis (diameter and pore size), as well as antimicrobial and anti-inflammatory activities. Results showed that the PVA/HC/EEHP materials, fabricated under controlled conditions of temperature and humidity, generated fiber membranes with diameters between 140–390 nm, adequate porosity and pore size for cell growth (67–90% and 4–16 µm, respectively), and good barrier properties (0.005–0.032 g·m−2 s−1) to be used in the treatment of conditions on the skin, and was even better than some commercial products. Finally, they showed to have anti-inflammatory (>80%), and antimicrobial activity against S. aureus and S. Epiderm. Furthermore, higher crystalline structure was observed according to the EEHP concentration. In addition, this is the first report in which PVA/HC/EEHP membranes are successfully fabricated and characterized.

Effect of Citric Acid on Swelling Resistance and Physicochemical Properties of Post-Crosslinked Electrospun Polyvinyl Alcohol Fibrous Membrane

“…By contrast, Kim et al [ 42 ] studied the attachment and serial proliferation rates of human prostate epithelial cells on electrospun polycaprolactone mats containing PVA, and the introduction of PVA increased the proliferation rate of the cells. In another study, fibroblasts were grown on PVA-based electrospun biomembranes; the results showed that PVA supported cell growth and proliferation [ 43 , 44 ]. In conclusion, PVA was chosen in combination with other materials as a barrier or facilitator layer for use as a GTR membrane to provide mechanical support and prevent rapid epithelial attachment or promote target cell proliferation.…”

Characterization and Applications of the Pectin Extracted from the Peel of Passiflora tripartita var. mollissima

“…The experiments were performed in independent triplicates. In this analysis, the denaturation temperature (TD), the decomposition temperature (TDS), the melting temperature (Tm) and the glass transition temperature (Tg) are reported [ 36 ].…”

Electrospun PVA Fibers for Drug Delivery: A Review

“…PVA with different drug formulations has been reported by various research groups for its wound-healing potential. A. García-Hernández et al reported PVA-based biomembranes with hydrolyzed collagen (HC) and ethanolic extract for their wound dressing characteristics [ 84 ]. Different concentrations of ethanolic extract of Hypericum perforatum ( EEHP ) in PVA/HC were evaluated.…”