PVA-CO-AAM and peg-co-aam hydrogels as bromelain carriers

Coco, Julia Cedran; Tundisi, Louise Lacalendola; Santos, Érica Mendes dos; Fava, Ana Laura Masquetti; Alves, Thaís Francine Ribeiro; Ataide, Janaína Artem; Chaud, Marco V.; Mazzola, Priscila Gava

doi:10.1016/j.jddst.2021.102483

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…Through comparing the R-squared value, the Dox release profile from the hydrogel in PBS showed a relatively better degree of fitting to the Korsmeyer–Peppas model. It is noteworthy that the R-squared value was reduced for Dox release in the presence of proteinase K. Additionally, the IgG release from the hydrogel did not fit well with these kinetic models, which may be attributed to the fact that the IgG release behavior was influenced by complicated interactions between the protein and hydrogel network [ 46 ]. Therefore, the drug release tests in vitro suggested that the drug-loaded hydrogels exhibited continuous release profiles of both Dox and antibodies, which could be adjusted by the hydrogel degradation.…”

Section: Resultsmentioning

confidence: 99%

Injectable Polypeptide Hydrogel Depots Containing Dual Immune Checkpoint Inhibitors and Doxorubicin for Improved Tumor Immunotherapy and Post-Surgical Tumor Treatment

et al. 2023

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In this work, we developed a strategy for local chemo-immunotherapy through simultaneous incorporation of dual immune checkpoint blockade (ICB) antibodies, anti-cytotoxic T-lymphocyte-associated protein 4 (aCTLA-4) and anti-programmed cell death protein 1 (aPD-1), and a chemotherapy drug, doxorubicin (Dox), into a thermo-gelling polypeptide hydrogel. The hydrogel encapsulating Dox or IgG model antibody showed sustained release profiles for more than 12 days in vitro, and the drug release and hydrogel degradation were accelerated in the presence of enzymes. In comparison to free drug solutions or hydrogels containing Dox or antibodies only, the Dox/aCTLA-4/aPD-1 co-loaded hydrogel achieved improved tumor suppression efficiency, strengthened antitumor immune response, and prolonged animal survival time after peritumoral injection into mice bearing B16F10 melanoma. Additionally, after injection of Dox/aCTLA-4/aPD-1 co-loaded hydrogel into the surgical site following tumor resection, a significantly enhanced inhibition on tumor reoccurrence was demonstrated. Thus, the polypeptide hydrogel-based chemo-immunotherapy strategy has potential in anti-tumor therapy and the prevention of tumor reoccurrence.

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Section: Resultsmentioning

confidence: 99%

Injectable Polypeptide Hydrogel Depots Containing Dual Immune Checkpoint Inhibitors and Doxorubicin for Improved Tumor Immunotherapy and Post-Surgical Tumor Treatment

et al. 2023

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“…5,10 The poly(vinyl alcohol) (PVA) hydrogel is a promising synthetic material for numerous biomedical applications due to its attractive features such as suitable mechanical properties, 11 availability, 12 biocompatibility, 13 nontoxicity, 14 and biodegradability. 15 However, the absence of bioactivity prevents PVA hydrogels from endothelialization in situ after implantation and may impair the longterm patency of the vascular grafts. 16,17 To enhance the biological activity of PVA hydrogels, researchers have performed various functional modifications on PVA.…”

Section: Introductionmentioning

confidence: 99%

“…There is a growing number of studies focused on developing artificial vascular grafts using hydrogel materials . However, traditional hydrogels are generally brittle and soft and are not capable of meeting the mechanical performance of artificial blood vessels, and their poor blood compatibility also remains a major obstacle in the application of vascular grafts. , The poly(vinyl alcohol) (PVA) hydrogel is a promising synthetic material for numerous biomedical applications due to its attractive features such as suitable mechanical properties, availability, biocompatibility, nontoxicity, and biodegradability . However, the absence of bioactivity prevents PVA hydrogels from endothelialization in situ after implantation and may impair the long-term patency of the vascular grafts. , To enhance the biological activity of PVA hydrogels, researchers have performed various functional modifications on PVA.…”

Section: Introductionmentioning

confidence: 99%

Dynamically Cross-Linked Double-Network Hydrogels with Matched Mechanical Properties and Ideal Biocompatibility for Artificial Blood Vessels

Jia,

Huang,

Meng

et al. 2024

ACS Appl. Mater. Interfaces

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Vessel transplantation is currently considered the "gold standard" treatment for cardiovascular disease. However, ideal artificial vascular grafts should possess good biocompatibility and mechanical strength that match those of native autologous vascular tissue to promote in vivo tissue regeneration. In this study, a series of dynamic cross-linking double-network hydrogels and the resultant hydrogel tubes were prepared. The hydrogels (named PCO), composed of rigid poly(vinyl alcohol) (PVA), flexible carboxymethyl chitosan (CMCS), and a cross-linker of aldehydebased β-cyclodextrin (OCD), were formed in a double-network structure with multiple dynamical cross-linking including dynamic imine bonds, hydrogen bonds, and microcrystalline regions. The PCO hydrogels exhibited superior mechanical strength, good network stability, and fatigue resistance. Additionally, it demonstrated excellent cell and blood compatibility. The results showed that the introduction of CMCS/OCD led to a significant increase in the proliferation rate of endothelial cells seeded on the surface of the hydrogel. The hemolysis rate in the test was lower than 0.3%, and both protein adsorption and platelet adhesion were reduced, indicating an excellent anticoagulant function. The plasma recalcification time test results showed that endogenous coagulation was alleviated to some extent. When formed into blood vessels and incubated with blood, no thrombus formation was observed, and there was minimal red blood cell aggregation. Therefore, this novel hydrogel tube, with excellent mechanical properties, exhibits antiadhesive characteristics toward blood cells and proteins, as well as antithrombotic properties, making it hold tremendous potential for applications in the biomedical and engineering fields.

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PVA-CO-AAM and peg-co-aam hydrogels as bromelain carriers

Cited by 2 publications

References 39 publications

Injectable Polypeptide Hydrogel Depots Containing Dual Immune Checkpoint Inhibitors and Doxorubicin for Improved Tumor Immunotherapy and Post-Surgical Tumor Treatment

Injectable Polypeptide Hydrogel Depots Containing Dual Immune Checkpoint Inhibitors and Doxorubicin for Improved Tumor Immunotherapy and Post-Surgical Tumor Treatment

Dynamically Cross-Linked Double-Network Hydrogels with Matched Mechanical Properties and Ideal Biocompatibility for Artificial Blood Vessels

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