Pva-miR-252 participates in ammonia nitrogen-induced oxidative stress by modulating autophagy in Penaeus vannamei

Wang, Feifei; Huang, Lin; Liao, Meiqiu; Dong, Wenna; Liu, Can; Zhuang, Xueqi; Liu, Yuan; Yin, Xiaoli; Liang, QingJian; Wang, Weina

doi:10.1016/j.ecoenv.2021.112774

Cited by 22 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overproduc-tion of ROS can harm lipids, proteins, and DNA [41]. Previous studies have proved that the juvenile liver of Nile tilapia suffered oxidative stress after being exposed to NH 3 for 72 days [42], and the antioxidant enzymes of Penaeus vannamei were dramatically increased first and then decreased, showing obvious cell fragmentation and vacuoles in hepatic tubules after ammonia exposure [21]. In the present study, Lv-HO-1 expression considerably increased after ammonia-N stress.…”

Section: Discussionsupporting

confidence: 54%

“…Autophagy can reduce the damage caused by oxidative stress in order to protect cells and increase lifespan [48]. Previous studies showed that after 42 days of ammonia treatment, chicken heart cells showed a clear autophagy phenomenon [49], and miR-252-mediated-ammonia-N-induced autophagy in P. vannamei [21].…”

Section: Discussionmentioning

confidence: 99%

“…vannamei is an internationally known economic shrimp, widely farmed worldwide, especially in China. However, ammonia is the most common pollutant in aquaculture waters and is highly toxic to crustaceans, leading to oxidative stress [21] and an immune response [22]. High ammonia levels can seriously damage the hepatopancreas, induce apoptosis, and even lead to the death of shrimp [23].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

Huang

Yang

et al. 2022

Fishes

View full text Add to dashboard Cite

Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the HO-1 gene from Litopenaeus vannamei (Lv-HO-1) was identified. The open reading frame of Lv-HO-1 is 747 bp, encoding a peptide of 248 amino acids as well as a conserved HemO structural domain. Lv-HO-1 is 70–90% homological to crustaceans and about 50% homological to arthropods. The transcript levels of Lv-HO-1 were highest in the hepatopancreas and lower in other tissues. Knockdown of Lv-HO-1 led to structural destruction of the hepatopancreas. After ammonia exposure, Lv-HO-1 was significantly induced. Knockdown of Lv-HO-1 during ammonia exposure resulted in a significant decrease in antioxidant capacity and cellular autophagy levels compared to the control and increased apoptosis. The transcriptional levels of SOD and GSH-Px were considerably reduced (p < 0.05), as were the transcriptional levels of Atg3, Atg4, Atg5, and Atg10. The results indicated that Lv-HO-1 from L. vannamei can be induced by oxidative stress and may have important roles in regulating the host antioxidant system, reducing cell apoptosis.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

Huang

Yang

et al. 2022

Fishes

View full text Add to dashboard Cite

show abstract

“…Autophagy related genes were activated and starvation disrupted intestinal homeostasis in the Chinese perch [ 57 ]. miR-252 increased autophagy and antioxidant enzyme activity by targeting PI 3 K to enhance the tolerance of Penaeus vannamei to ammonia nitrogen stress [ 58 ], while few miRNA studies have reported on autophagy when fish starved. Future miRNA studies can focus on this area.…”

Section: Feedmentioning

confidence: 99%

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Cao

Zhang

et al. 2023

Biology

View full text Add to dashboard Cite

miRNAs are a class of endogenous and evolutionarily conserved noncoding short RNA molecules that post-transcriptionally regulate gene expression through sequence-specific interactions with mRNAs and are capable of controlling gene expression by binding to miRNA targets and interfering with the final protein output. The miRNAs of teleost were firstly reported in zebrafish development, but there are recent studies on the characteristics and functions of miRNAs in fish, especially when compared with mammals. Environmental factors including salinity, oxygen concentration, temperature, feed, pH, environmental chemicals and seawater metal elements may affect the transcriptional and posttranscriptional regulators of miRNAs, contributing to nearly all biological processes. The survival of aquatic fish is constantly challenged by the changes in these environmental factors. Environmental factors can influence miRNA expression, the functions of miRNAs and their target mRNAs. Progress of available information is reported on the environmental effects of the identified miRNAs, miRNA targets and the use of miRNAs in fish.

show abstract

“…Rapamycin is an inhibitor of the mTOR signaling pathway and is usually used as an inducer of autophagy (Sotthibundhu, et al 2016). Our previous studies have shown that autophagy plays an important role in the environmental stress of shrimp (Liang, et al 2021a;Liang, et al 2021b;Wang, et al 2020;Wang, et al 2021). In this study, we aimed to explore the role of autophagy in shrimp response to the environment by administering rapamycin intramuscularly to shrimp and to understand the molecular mechanisms of autophagy to expand our understanding of shrimp adaptation to environmental challenges.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Lin,

Liu,

Chen

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Nitrite constitutes a significant pollutant that impedes the growth of P. vannamei. Conventional treatments are primarily geared toward decreasing nitrite concentrations from an environmental standpoint. Nevertheless, investigating methodologies to enhance the ability of shrimp to withstand nitrite-induced stresses on an individual basis remains an unexplored topic. The present study examines the impact of autophagy on shrimp’s response to high nitrite environments through rapamycin injection. The research findings indicate that activating autophagy can effectively enhance the survival rate of shrimp under high nitrite conditions. Additionally, total hemocyte count (THC) results in the hemolymph demonstrate that autophagy can alleviate the reduction in blood cell count caused by nitrite stress. The transcriptome results show that up-regulation of 718 genes and down-regulation of 1,071 genes after nitrite stress. Most of the differentially expressed genes (DEGs) after nitrite stress were related to physiological processes, including oxidative stress, energy metabolism, and lysosomes. Compared with nitrite stress, there are 911 up-regulated genes and 713 down-regulated genes activated by autophagy, which are related to immune response, specifically involving glutathione metabolism, drug metabolism-cytochrome P450, and metabolism of xenobiotics by cytochrome P450, lysosomes, autophagy, phagosomes, and MAPK signaling pathways. These pathways can reduce oxidative stress and promote the metabolism of harmful substances in the body, in shrimp, thereby improving their survival rates. These findings provide new insights for research aimed at enhancing nitrite tolerance in shrimp.

show abstract

Pva-miR-252 participates in ammonia nitrogen-induced oxidative stress by modulating autophagy in Penaeus vannamei

Cited by 22 publications

References 45 publications

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Contact Info

Product

Resources

About