PVI-25 The effect of autoantibodies to test results in patients with low-titre anti-HCV positivity

Zer, Yasemin; Ekşi, Fatma; Balcı, İclal; Bayram, Arzu; Karslığil, Tekin

doi:10.1016/s1386-6532(09)70174-x

Cited by 2 publications

(2 citation statements)

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“…Sarıbaş ve arkadaşlarının ( 7) çalışmasında da yaş gruplarına bakıldığında en yüksek oran 40 yaş üzerinde bulunmuştur; Asan ve arkadaşlarının (9) çalışmasında ise hastaların %54'ünün 50 yaş ve üzeri olduğu görülmüştür. Bu sonuçlar bizim çalışmamızla uyumlu olup yaş ile birlikte artan, özellikle düşük düzey anti-HCV pozitifliğinin geçirilmiş infeksiyona bağlı olabileceği de öne sürülmektedir (10). TURHEP çalışmasında, risk faktörleri açısından erkek cinsiyet, düşük eğitim düzeyi, evli olmak, Güneydoğu Anadolu bölgesinde yaşamak, hepatitli hasta ile yakın temas, ortodental müdahaleler ve daha önce tek kullanımlık olmayan enjektör kullanımı incelenmiş ve artan HCV prevalansı ile ilişkili tek risk faktörünün 50 yaş üzerinde olmak olduğu bildirilmiştir (4).…”

Section: İrdelemeunclassified

Retrospective Evaluation of Serological and Molecular Tests for Determination of Anti-HCV Screening Test Cut-Off

Ozer-Yildirim,

Nalca-Erdin,

Kula-Atik

2023

Klimik Derg

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Objective: We aimed to determine an appropriate anti-HCV test cut-off value for the HCV-RNA study and contribute to the development of correct algorithms by evaluating our hospital’s anti HCV and HCV-RNA test results between 2017-2019. Methods: Available HCV-RNA test results of patients who were found to have reactivity in anti-HCV were analyzed. Patients with anti-HCV reactivity were analyzed by dividing into age groups 0-20 years, 21-40 years, 41-60 years, and ≥61 years. HCV-RNA results were also evaluated within the same age groups. Patients with anti-HCV reactivity were divided into three groups according to their S/Co values as 1-5 mLU/mL, 5.1-10 mLU/mL, and ≥10.1 mLU/mL, and statistical analysis was performed. Results: Anti-HCV reactive results were divided into groups of 1.0-3.8 mLU/mL and ≥3.9 mLU/mL according to S/Co values, and HCV-RNA positivity was 2% and 44.4% respectively. When divided into three groups as 1-5 mLU/mL, 5.1-10 mLU/mL, and ≥10.1 mLU/mL according to S/Co values, the HCV RNA positivity rate was 1.8%, 25%, and 55.2%, respectively. The difference between the groups was statistically significant. Conclusion: Countries should carry out comprehensive studies to represent their populations to determine cut-off values for widely used kits and to make this applicable with algorithms.

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Section: İrdelemeunclassified

Retrospective Evaluation of Serological and Molecular Tests for Determination of Anti-HCV Screening Test Cut-Off

Ozer-Yildirim,

Nalca-Erdin,

Kula-Atik

2023

Klimik Derg

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“…The plasma viral load in HCV infection was often very low in large number of patients [5,6], much below the validated calibration plots for quantitative real time PCR (RT-qPCR). There were many patients with very low reactive anti-HCV antibody levels [7], which could be detected only with instruments having enhanced chemiluminescence detection system. There were di culties in interpretation of the large number of third generation anti-HCV reactive antibody screening assays which were RT-PCR negative.…”

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confidence: 99%

Data Analysis and Graphical observations of real-time RT-PCR of Hepatitis C Virus to determine the range of qualitative assays and to differentiate it from quantitative assays

Joseph

Yathi

Jacob

2022

Preprint

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Hepatitis C Virus (HCV) load in plasma were often below the validated quantitative RT-PCR (RT-qPCR) calibration plot concentrations. In this report, data analysis of RT-PCR amplification plots and calibration plots were done to define the performance characteristics of the higher Cq range of qualitative RT-PCR and to differentiate it from RT-qPCR. This is the first report to establish the decision criteria for validation of qualitative real-time HCV RT-PCR assays. Patients reactive for anti-HCV antibody screening assays or exposed to HCV infection were selected for RT-PCR assay done with RNA isolated from plasma. Normalised fluorescence intensity (100%) and slope (1.65–1.50) of the sigmoid fluorescence amplification plots were of acceptable performance for validated quantitative calibrators (10,000–10 IU/µl), but were decreased for diluted calibrators and for patients’ sample with Cq greater than that of the 10 IU/µl validated calibrator (Cq > 33.2). Calibration plot of validated calibrators had slope, efficiency and sensitivity of -3.471, 94% and 36.404, respectively, but the diluted calibrators showed decreased performance of -2.305, 171% and 35.52, respectively. Log-transformed exponential amplification plots analysed by LinRegPCR software, showed acceptable performance at the validated calibrator concentrations and decreased performance with diluted calibrators and patients’ sample with Cq > 33.2. The four-parameter validated sigmoid fluorescence amplification plot of qualitative RT-PCR Cq range was from 33.2 to 39.9. The 10 IU/µl calibrator was the reference point to differentiate between qualitative and quantitative RT-PCR. A systematic decision-criteria had been developed for this purpose from the data analysis of graphical observations, four-parameter sigmoid curve fitting model, exponential amplifications of individual RT-PCR plots and calibration plots.

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PVI-25 The effect of autoantibodies to test results in patients with low-titre anti-HCV positivity

Cited by 2 publications

References 0 publications

Retrospective Evaluation of Serological and Molecular Tests for Determination of Anti-HCV Screening Test Cut-Off

Retrospective Evaluation of Serological and Molecular Tests for Determination of Anti-HCV Screening Test Cut-Off

Data Analysis and Graphical observations of real-time RT-PCR of Hepatitis C Virus to determine the range of qualitative assays and to differentiate it from quantitative assays

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