Pycnidione, a fungus-derived agent, induces cell cycle arrest and apoptosis in A549 human lung cancer cells

Hsiao, Chiaolong; Hsiao, Shih-Hsin; Chen, Weilin; Guh, Jih‐Hwa; Hsiao, George; Chan, Yen-Ju; Lee, Tzong‐Huei; Chung, Chi-Li

doi:10.1016/j.cbi.2012.03.004

Cited by 39 publications

(29 citation statements)

References 61 publications

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“…The distribution of cells (10 4 ) in different phases of cell cycle was examined using a BD FACSAria III flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). The results were analyzed using the FlowJo software 36 . The experiment was repeated for 4 independent times (n = 4).…”

Section: Methodsmentioning

confidence: 99%

Radiosensitizing effect of ellagic acid on growth of Hepatocellular carcinoma cells: an in vitro study

Das

Biswas

Chattopadhyay

et al. 2017

Sci Rep

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Failure of treatment for cancer in clinic by radio/chemotherapy is generally attributed to tumour resistance. Therefore, it is important to develop strategies to increase the cytotoxicity of tumour cells by radiation in combination with unique tumour selective cytotoxic agents. We evaluated the potential of ellagic acid (EA) as an enhancer of oxidative stress in cancer cells. HepG2 cells were treated with EA (10 µM) for 12 h prior to exposure of single 7.5 Gy dose of irradiation. Treatment of HepG2 cells with EA and gamma radiation showed increased reactive oxygen species generation, up regulation of p53 protein expression, decreased survival markers level like p-Akt, p-NF-kB and p-STAT3 which were significantly higher after radiation treatment alone. We also found that combination treatment increased G2/M phase cell population, decreased IL-6, COX–2 and TNF-α expression and caused a loss in mitochondrial membrane potential with decreased level of angiogenesis marker MMP-9. Over expression of Bax and activation of caspase 3 indicated the apoptosis of the cells. The results provided a strong unique strategy to kill cancer cells HepG2, using less radiation dose along with effective pro-oxidant dose of EA.

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Section: Methodsmentioning

confidence: 99%

Radiosensitizing effect of ellagic acid on growth of Hepatocellular carcinoma cells: an in vitro study

Das

Biswas

Chattopadhyay

et al. 2017

Sci Rep

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“…The percentage of DNA content in each phase of the cell cycle was evaluated by BD FACSAria III flow cytometer (Becton Dickinson, Franklin Lakes, NJ). The data were analysed using Flow Jo Software (Version 10.0) 52 .…”

Section: Methodsmentioning

confidence: 99%

Smokeless tobacco consumption impedes metabolic, cellular, apoptotic and systemic stress pattern: A study on Government employees in Kolkata, India

Biswas

Manna

Das

et al. 2015

Sci Rep

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Smokeless tobacco (SLT) remains a threat amongst a large population across the globe and particularly in India. The oral use of tobacco has been implicated to cause physiological stress leading to extreme toxicological challenge. The study included 47 SLT-users and 44 non-users providing a spectrum of pathophysiological, clinico-biochemical, antioxidant parameters, cell cycle progression study of PBMC and morphological changes of red blood cells (RBC). The expressions of p53, p21, Bax, Bcl-2, IL-6, TNF- α, Cox-2, iNOS were analyzed from thirteen representative SLT-users and twelve non-users. Difference in CRP, random glucose, serum cholesterol, TG, HLDL-C, LDL-C, VLDL-C, neutrophil count, monocyte count, ESR, SOD (PBMC) and TBARS (RBC membrane) were found to be statistically significant (p < 0.05) between the studied groups. The current study confers crucial insight into SLT mediated effects on systemic toxicity and stress. This has challenged the metabolic condition leading to a rise in the inflammatory status, increased apoptosis and RBC membrane damage. The above findings were substantiated with metabolic, clinical and biochemical parameters. This is possibly the first ever in-depth report and remains an invaluable document on the fatal effects of SLT.

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“…Cell cycle deregulation is one of the major hallmarks of cancer progress, and induction cell cycle arrest is an important cause to inhibit cancer growth and proliferation (Hsiao et al, 2012). NAT-F showed different effect on cell cycle arrest, induced S stage arrest in PC9 cells, but G 0 /G 1 stage arrest in H1299 cells, which suggested that NAT-F is a cell cycle non-specific anti-tumor compound.…”

Section: Discussionmentioning

confidence: 99%

Neoantimycin F, a Streptomyces-Derived Natural Product Induces Mitochondria-Related Apoptotic Death in Human Non-Small Cell Lung Cancer Cells

Liu

Zhu

et al. 2019

Front. Pharmacol.

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Streptomyces-derived natural products have been become a major focus of anti-tumor drug discovery studies. Neoantimycin F (NAT-F), was isolated from Streptomyces conglobatus by our group. Here, we examined the anti-cancer activities and its underlying molecular mechanisms implicated in NAT-F–induced apoptosis of non–small cell lung cancer (NSCLC) cells. Our results showed that NAT-F exerted excellent growth-inhibitory activity against PC9 and H1299 cells in a concentration-dependent manner. NAT-F–induced cell cycle arrest at S and G0/G1 phase in PC9 and H1299 cells, respectively. Further investigation revealed that the key proteins (including cyclinD1, cyclinE1, cyclinB1, CDK2, and CDK4) were involved in the cell regulation by NAT-F. Additionally, NAT-F significantly increased the production of reactive oxygen species (ROS), induced DNA damage, nuclear condensation, and cell apoptosis in both cell lines. Moreover, loss of the mitochondrial membrane potential (MMP) was markedly induced by NAT-F. Additional results revealed that NAT-F could up-regulate pro-apoptotic protein Bax and down-regulate anti-apoptotic protein Bcl-2, Mcl-1, and Bcl-xL, resulting in cytochrome c release from mitochondria and sequential activation of caspase-9 and -3, as well as the cleavage of poly (ADP-ribose) polymerase. Meanwhile, c-Jun N-terminal kinase (JNK), p38 MAPK (p38), and extracellular signal-regulated kinase (ERK) signaling pathway were also involved in anti-cancer activity of NAT-F in NSCLC cells. Taken together, these findings indicated that NAT-F possessed anti-proliferative effect and induced apoptosis in NSCLC cells in vitro and may be conducive to promote the development of novel anti-NSCLC agents.

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Pycnidione, a fungus-derived agent, induces cell cycle arrest and apoptosis in A549 human lung cancer cells

Cited by 39 publications

References 61 publications

Radiosensitizing effect of ellagic acid on growth of Hepatocellular carcinoma cells: an in vitro study

Radiosensitizing effect of ellagic acid on growth of Hepatocellular carcinoma cells: an in vitro study

Smokeless tobacco consumption impedes metabolic, cellular, apoptotic and systemic stress pattern: A study on Government employees in Kolkata, India

Neoantimycin F, a Streptomyces-Derived Natural Product Induces Mitochondria-Related Apoptotic Death in Human Non-Small Cell Lung Cancer Cells

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