Pyomelanin-Producing Brevundimonas vitisensis sp. nov., Isolated From Grape (Vitis vinifera L.)

Abstract

A novel endophytic bacterial strain, designated GR-TSA-9T, was isolated from surface-sterilized grape (Vitis vinifera L.). 16S rRNA gene sequence analyses showed that the isolate was grouped within the genus Brevundimonas, displaying the highest similarity with Brevundimonas lenta DS-18T (97.9%) and Brevundimonas kwangchunensis KSL-102T (97.8%) and less than 97.5% similarity with other members of Brevundimonas. The strain GR-TSA-9T was a gram negative, rod shaped, facultatively anaerobic, catalase and oxidase positive, and motile bacterium. Its growth occurred at 10–37°C (optimally 25–30°C), at pH 7.0–8.0, and in NaCl 0–1% (optimally 0%). It contained ubiquinone-10 as a respiratory quinone, and the major cellular fatty acids (>10% of the total) were C16:0 (14.2%) and summed feature 8 (C18:1ω7c and/or C18:1ω6c, 65.6%). The polar lipids present in the strain were phosphoglycolipids, phosphatidylglycerol, 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-α-d-glucopyranuronosyl]glycerol, and unidentified lipids (L1, L2, and L4). The strain had one 2,976,716bp circular chromosome with a G+C content of 66.4%. The digital DNA–DNA hybridization value between strain GR-TSA-9T and B. lenta DS-18T was 20.9%, while the average nucleotide identity value was 76.7%. In addition, the dDDH and ANI values to other members in this genus, whose genome sequences are available, are less than 21.1 and 77.6%. Genome annotation predicted the presence of some gene clusters related to tyrosine degradation and pyomelanin formation. Strain GR-TSA-9T produced a brown melanin-like pigment in the presence of L-tyrosine-containing media. The highest pigment production (0.19g/L) was observed in tryptic soy broth with 1.0mg/ml L-tyrosine at 25°C for 6days of culture. Biophysical characterization by ultraviolet (UV)–visible spectroscopy, Fourier-transform infrared spectroscopy, and electrospray ionization mass spectrometry confirmed that the pigment was pyomelanin. Additionally, melanized GR-TSA-9T cells could protect the cells against UVC exposure. The phylogenetic, genomic, phenotypic, and chemotaxonomic features indicated that strain GR-TSA-9T represents a novel melanin-producing species of Brevundimonas. The type strain was GR-TSA-9T (KCTC 82386T=CGMCC 1.18820T).

Resourcefulness of propylprodigiosin isolated from Brevundimonas olei strain RUN-D1

“…Brevundimonas have been utilised in soil bioremediation (Zhang et al 2021 ) and in treating polluted water (Wang et al 2015 ). Recently, Jiang et al ( 2021 ) reported the isolation of a novel pyomelanin-producing B . vitisensis from a grape; however, this is the first study to report the isolation of a novel red pigment from Brevundimonas .…”

Sphingomonas cannabina sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’

“…The growth of strain DM2-R-LB4 T was observed in different media, namely LBA, marine agar 2216 (MA, Difco), malt extract agar (MEA, Difco), potato dextrose agar (PDA, Difco), Reasoner's 2A agar (R2A, MB cell), nutrient agar (NA, Difco), ISP2 (yeast extract 4.0 g l -1 , malt extract 10.0 g l -1 , dextrose 4.0 g l -1 , agar 20.0 g l -1 ) and trypticase soy agar (TSA, Difco). Growth on TSA medium was observed at different temperatures (4,10,15,20,25,30,35,37,40,45, 50 and 55 °C) and different pH (pH 4.0-10.0, at intervals of 0.5 pH unit). Two separate buffers were used for studying growth at different values of pH.…”

“…Polyamines were extracted from the freeze-dried cells and analysed as described by Busse and Auling [34]. The polar lipids were extracted from 100 mg of freeze-dried cells according to the method described by Jiang et al [35]. The polar lipids were separated by two-dimensional thin-layer chromatography on 20×20 cm silica gel plates (Merck) and identified by spraying with phosphomolybdic acid, molybdenum blue, and ninhydrin [36].…”

Genome-Based Insights into the Thermotolerant Adaptations of Neobacillus endophyticus BRMEA1T

“…This research highlights the potential use of thermotolerance genes and SM-BCGs to enhance stress tolerance and engineer crops resistant to various challenges. For example, previous studies explored bacterial strains producing pyomelanin, which aids grapes in preventing UV damage (Jiang et al, 2021), and nostoxanthin, known to reduce reactive oxygen species and improve salt stress (Jiang et al, 2023). These findings indicate that the BRMEA1T strain might play a significant role in facilitating thermotolerance adaptation in host plants.…”