Pyometra in cattle: distribution and therapy

Nikolaev, S.V.; Konopeltsev, I.G.

doi:10.30896/0042-4846.2020.23.12.39-43

Cited by 2 publications

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“…На фоне роста продуктивности у молочных коров обострилась проблема распространения бесплодия [8,9]. Основными причинами снижения воспроизводительной функции у крупного рогатого скота, как правило, являются дисгормональные расстройства, которые возникают на фоне метаболических нарушений и патологий органов внутренней секреции [3,5,6]. Поэтому оценка гуморального статуса животных является одним из способов прогнозирования бесплодия и выбора объективной тактики коррекции гормональных нарушений [4,7,10].…”

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Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Nikolaev¹

2022

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The optimal conditions for determining the concentration of progesterone in the blood plasma of cattle were selected by high-performance liquid chromatography-tandem mass spectrometry, and the relationship of the level of this progestin with fertilization after treatment of cows with hormonal preparations was studied. It was found that the time of progesterone peak release under the selected chromatography mode is within 2.7 minutes, and the optimal precursor ion is [M+H]+ m/z=315.3. The developed technique makes it possible to determine the endogenous level of the steroid in the blood of cattle with high accuracy (up to hundredths of ng/ml). Analysis of progesterone concentration in cows at the time of the final injection of the prostaglandin analogue showed that animals with different reproductive status have no significant differences in the content of this steroid. The most statistically significant indicators were obtained on the day of insemination. Thus, in infertile cows, the hormone concentration was 1.9...4.2 times lower than in fertilized cows (P<0.05). The higher steroid content in fertilized animals at the time of insemination most likely reflects the ovarian response to the preovulatory release of luteinizing hormone and the readiness of the follicle for ovulation. Keywords: cattle; high-performance liquid chromatomass spectrometry; progesterone; synchronization of sexual cyclicity; fertilization; infertility.

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Section: Original Articleunclassified

Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Nikolaev¹

2022

asj

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Determination of the concentration of selective progesterone receptor modulators in blood plasma BY LC–MS/MS

Nikolaev

2022

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High-performance liquid chromatography mass spectrometry is a hybrid method and combines independent processes of liquid chromatographic separation and mass spectrometric analysis, which makes it possible to effectively determine the concentration of various substances in complex substances. The optimal parameters for the determination of progesterone, 17-hydroxyprogesterone capronate (17-OHPC) and aglepriston in the blood plasma of cattle by this method have been established in the work. The quantitative determination of the components was carried out by the method of an external standard using reference substances and preliminary construction of calibration dependencies (absolute calibration). To account for the effect on the result of the analysis of various components of blood plasma, the matrix calibration method was used using "pure" plasma, i.e., it did not contain detectable compounds. It was experimentally determined that the time of progesterone peak release is within 2.72 minutes, and the optimal precursor ion is m/z =315.3. In the course of the work carried out, it was found that in the blood plasma of animals, endogenous progesterone is well detected by the developed method according to the parameters set for the synthetic analogue. The peak time of 17-OHPC occurred after 4.20 minutes, and the optimal precursor ion had a mass of 429.2 au. The first stage of studying the kinetics of 17-HPC in cattle showed that the steroid is well visualized on the chromatogram even in low concentrations. The outputs of the peaks of aglepriston were observed at 2.25 minutes, and the optimal precursor ion was equal to 432.2 au.m. The chromatogram even at low concentrations of the steroid had a low noise level, and the concentration of the steroid in the blood plasma a day after injection of 20 ml of 3% aglepriston solution to a lactating cow was 7.2 ng/ml. Thus, optimal parameters were selected for determining the concentration of selective progesterone receptor modulators in the blood plasma of cattle, which allows for further evaluation of the pharmacokinetic properties of these steroids after their use.

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Pyometra in cattle: distribution and therapy

Cited by 2 publications

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Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Determination of the concentration of selective progesterone receptor modulators in blood plasma BY LC–MS/MS

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