Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Abstract

a b s t r a c tMethods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mM and the limit of detection (LOD) was 0.5 mM. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples.

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

“…Crude pyoverdine was obtained from Pseudomonas aeruginosa PA1, and the isolation method had been reported in our previous work [22]. The crude pyoverdine was firstly purified by copperchelate chromatography.…”

“…It helps Pseudomonas aeruginosa uptake iron to overcome iron limitation as well as protects them from heavy metal toxicity [20]. As the excellent fluorescence property of pyoverdine, it has already been utilized to established biosensors for the detection of dissociative ferric ion and furazolidone [21,22]. In this work, we have discovered that the fluorescence of pyoverdine can be quenched obviously in the presence of copper ion under neutral condition.…”

Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1

“…The earlier study shows that mercury-resistant strain Pseudomonas aeruginosa owns excellent mercury adsorption capacity (Chang and Hong, 1994). Our previous study illustrates that the carboxylesterase E2 from Pseudomonas aeruginosa PA1 can well adsorb Hg 2þ (Yin et al, 2014 . Therefore, the objective of this study is to develop a multifunctional surface display system by using carboxylesterase E2 from Pseudomonas aeruginosa PA1 to achieve simultaneous remediation and detection of Hg .…”

“…It is known that mercuryresistant strain Pseudomonas aeruginosa owns excellent mercury removal ability (Chang and Hong, 1994). The Pseudomonas aeruginosa PA1 has already been isolated in our previous work (Yin et al, 2014). As a bio-element secreted from Pseudomonas aeruginosa PA1, carboxylesterase E2 can also adsorb Hg 2þ with satisfied adsorption capacity.…”

Electrochemical degradation of nitrofurans furazolidone by cathode: Characterization, pathway and antibacterial activity analysis

“…Thus, its utilization has been banned in food-producing animals by European Union and Ministry of Agriculture of China in 1995 and 2002, respectively. However, due to the low production cost and high effectiveness of FZD, it is still being illegally used in a relatively large scale across the world, particularly in developing countries [6]. In general, animals and human had a very low assimilation rate of the FZD, thus a majority of the FZD used in farms and aquaculture was finally released into the environments [4].…”