Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production

Abstract

Rice blast caused by pathogenic fungus Magnaporthe oryzae is one of the most serious diseases in rice. The pyramiding of effective resistance genes into rice varieties is a potential approach to reduce the damage of blast disease. In this study, combinations of three resistance genes, Pigm, Pi48 and Pi49, were introduced into a thermo-sensitive genic male sterile (PTGMS) line Chuang5S through marker-assisted selection. The results showed that the blast resistance of improved lines increased significantly compared with Chuang5S, and the three gene pyramiding lines (Pigm + Pi48 + Pi49) had higher rice blast resistance level than monogenic line and digenic lines (Pigm +Pi48, Pigm + Pi49). The genetic backgrounds of the improved lines were highly similar (>90%) to the recurrent parent Chuang5S by using the RICE10K SNP chip. In addition, agronomic traits evaluation also showed pyramiding lines with two or three genes similar to Chuang5S. The yields of the hybrids developed from improved PTGMS lines and Chuang5S are not significantly different. The newly developed PTGMS lines can be practically used for th breeding of parental lines and hybrid varieties with broad spectrum blast resistance.

Development of Rice Lines by Pyramiding of Major Blast Resistance Genes

“…MAS analysis facilitates the identification of genes, their introduction and pyramiding into new lines [40,41], that significantly speeds up rice breeding. The combination of several genes in one genotype showed the greatest effect, indicating the effectiveness of the strategy of durable rice resistance to blast [7][8][9][10][11][12][13][14]35].…”

“…The combination of several genes in one genotype showed the greatest effect, indicating the effectiveness of the strategy of durable rice resistance to blast [7][8][9][10][11][12][13][14]35]. Rice lines carrying both individual resistance genes [40,42] and lines with pyramided genes (containing combinations genes) with a broad spectrum of resistance to Magnaporthe oryzae were created [41,43]. Thus, as a result of molecular screening of parental samples for the presence of genes, it was shown that the resistance of local zoned rice cultivars Bakanasski, Fatima, Aisaule and Aru, out of the four genes studied, only one gene Pi-2 is present and only in the cultivars Bakanasski, Aisaule and Aru.…”