Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight

Maruthasalam, S.; Kalpana, K.; Kumar, K. K.; Loganathan, M.; Poovannan, K.; Raja, Joseph A. J.; Kokiladevi, E.; Samiyappan, R.; Sudhakar, D.; Balasubramanian, P.

doi:10.1007/s00299-006-0292-5

Cited by 81 publications

(43 citation statements)

References 63 publications

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“…In this case, the data also indicate that this strong effect is only due to the combined action of the thaumatin-like and chitinase proteins, independently of the Xa21 gene (Maruthasalam et al 2007). Some excellent levels of protection were also shown for kernel smut (caused by Tilletia barclayana; Zhu et al 2007) by pyramiding (Table 4).…”

Section: Disease Protection Quantitative Effectsmentioning

confidence: 80%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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Diseases caused by fungal and bacterial pathogens like Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae are responsible for considerable yield loss. Up to now, in rice, the modification of the expression of more than 60 genes from diverse origins has shown beneficial effects with respect to disease resistance. In this paper, we review this large set of data to identify the best genes and strategies to achieve disease resistance by transgenic approaches. Altered expression of genes involved in signal transduction and transcription may lead to many unwanted side effects, like lesion mimic phenotypes. Moreover, modification of resistance to abiotic stress has been neglected and should be carefully examined in the future. Genes like resistance genes and pathogenesis-related genes can confer broad spectrum and high levels of resistance to several pathogens. Preformed expression of defense is often observed but does not necessarily lead to detrimental effects. Although examples of gene pyramiding are scarce, they suggest that this is a very promising strategy. More field evaluation of the transgenic plants is required to draw final conclusions on the usefulness of these genes for improving disease resistance.

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Section: Disease Protection Quantitative Effectsmentioning

confidence: 80%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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“…Hence, it is possible that increased resistance in rice to R. solani after application of OA may be related to the accumulation of phenolics and defense related proteins. In recent years, there has been success in control of sheath blight by overexpression of antifungal proteins in rice plants through genetic engineering (Lin et al 1995;Datta et al 1999;Datta et al 2001;Kalpana et al 2006;Maruthasalam et al 2007;Shah et al 2009). However, it is suggested that the constitutive expression of defense genes may likely impose a considerable drain on energy and metabolic resources, whereas inducible defenses would impose a significantly lower metabolic cost (Herms and Mattson 1992;Heil and Bostock 2002).…”

Section: Discussionmentioning

confidence: 99%

Oxalic acid-induced resistance toRhizoctonia solaniin rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteins

Jayaraman

Bhuvaneswari

Rabindran

et al. 2010

Journal of Plant Interactions

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Oxalic acid (1 mM) when applied as a foliar spray to rice plants induced resistance to challenge infection with Rhizoctonia solani, the rice sheath blight pathogen. Maximum reduction in sheath blight incidence was observed when the plants were sprayed with oxalic acid three days before inoculation with the fungus. The biochemical alterations in rice plants treated with oxalic acid was also investigated. When rice plants were treated with oxalic acid, a two-fold increase in phenolic content in leaf sheaths was recorded three days after treatment. Phenylalanine ammonia-lyase and peroxidase activities increased significantly starting from two days after treatment. Peroxidase (PO) isozyme analysis indicated that PO-3 and PO-4 were induced two days after treatment with oxalic acid. Western blot analysis revealed that two chitinases (28 and 35 kDa) and two b-1,3-glucanases (30 and 32 kDa) were strongly induced in rice sheaths four to six days after treatment with oxalic acid. Immunoblot analysis of protein extracts from oxalic acid-treated plants demonstrated the induction of a 23 kDa thaumatinlike protein (TLP) cross-reacting with bean TLP antibody. These results suggest that the enhanced activities of defense enzymes and defense-related compounds in oxalic acid-treated rice plants may contribute to resistance against R. solani.

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“…PB1 and ADT38 using a biolistic method. The co-transformants exhibited an enhanced resistance against R. solani (Maruthasalam et al 2007). Transgenic rice plants of local cultivars developed through Agrobacterium-mediated transformation expressing rice chitinase gene RC7 exhibit an enhanced resistance (up to 33.3 %) to R. solani under glasshouse conditions (Nandakumar et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Rajesh¹,

Maruthasalam²,

Kalpana³

et al. 2016

Biologia plant.

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Development of transgenic plants by introducing defense genes is one of the strategies to engineer disease resistance. Transgenic ASD16 rice plants harbouring rice chitinase chi11 gene, belonging to a PR-3 group of defense gene conferring sheath blight (Rhizoctonia solani Kuhn) resistance, were used in this study. Three T 2 homozygous lines (ASD16-4-1-1, 5-1-1, and 6-1-1) were identified from seven putative (T 0 ) transgenic lines expressing chi11 using Western blotting analysis. The inheritance of sheath blight resistance in those lines was studied over generations. The stability of chi11 expression up to T 4 generation in all the three homozygous lines was proved by Western blot and the stability of sheath blight resistance in the homozygous lines was proved up to T 4 generation using detached leaf and intact leaf sheath assays. Among the three homozygous lines tested, ASD16-4-1-1 showed consistent results in all the generations and gave a better protection against the sheath blight pathogen than the other two lines.

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Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight

Cited by 81 publications

References 63 publications

Potential Candidate Genes for Improving Rice Disease Resistance

Potential Candidate Genes for Improving Rice Disease Resistance

Oxalic acid-induced resistance toRhizoctonia solaniin rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteins

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

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