Pyrenacantha volubilis Wight, (Icacinaceae) a rich source of camptothecine and its derivatives, from the Coromandel Coast forests of India

Suma, H.K.; Kumar, Vadlapudi; Umapathy, Senthilkumar; Kumara, P. Mohana; Ravikanth, G.; Santhoshkumar, T.R.; Shaanker, R. Uma

doi:10.1016/j.fitote.2014.05.017

Cited by 19 publications

(5 citation statements)

References 19 publications

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“…Different plant extracts containing various alkaloids were examined on in vitro anticancer activity e.g., Peganum harmala containing harmine derivative alkaloids [4], Pyrenacantha volubilis containing quinoline indole alkaloid camptothecine [5], Anthocephalus cadamba containing dihydrocadambine [6], Enicosanthellum pulchrum with cleistopholine [7], Ophiorrhiza mungos L. var. angustifolia containing anticancer alkaloid camptothecin [8], Ophiorrhiza rugosa var.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

et al. 2019

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Background: Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on various cancer cells. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. Objective: This study aimed to investigate of cytotoxic activity of extracts that were obtained from Chelidonium Majus; Berberis sp.; Thalictrum foetidum containing various alkaloids on selected cancer cell lines. The aim was also the quantification of selected alkaloids in the investigated extracts by HPLC. Methods: The analysis of alkaloids contents were performed while using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water, and ionic liquid. The cytotoxic effect of the tested plant extracts and respective alkaloids’ standards were examined while using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), and human triple-negative breast adenocarcinoma cell line (MDA-MB-231). Conclusion: All of the investigated plant extracts possess cytotoxic activity against cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu and MDA-MB-231 cells was observed for Chelidonium majus root extract, while the highest cytotoxic activity against SCC-25 and MCF-7 cells was estimated for the Thalictrum foetidum root extract. There obtained significant differences in the cytotoxic activity of extracts that were obtained from the roots and herbs of Chelidonium majus and Thalictrum foetidum. Based on these results, investigated plant extracts can be recommended for further investigations of anticancer activity.

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Section: Introductionmentioning

confidence: 99%

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

et al. 2019

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“…High-throughput sequencing facilitates research in CPT-producing plants Camptothecin is one of the most promising anticancer drugs with additional pharmacological properties including antiviral and antifungal activities (Wu and Chu, 2017;Hevener et al, 2018;Xu et al, 2020b). More than 10 CPT-producing plant species have been discovered from different families, such as Nyssaceae, Icacinaceae, Apocynaceae, and Rubiaceae (Ramesha et al, 2013;Suma et al, 2014;Pu et al, 2019;Pu et al, 2020;Ruan et al, 2021a). In the Ophiorrhiza genus of the Rubiaceae family, CPT-producing O. pumila is a representative folk medical plant (Yang et al, 2018;Taher et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

OpNAC1 transcription factor regulates the biosynthesis of the anticancer drug camptothecin by targeting loganic acid O‐methyltransferase in Ophiorrhiza pumila

Hao

Wang

Zhou

et al. 2023

JIPB

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Camptothecin (CPT) is an anticancer pentacyclic quinoline alkaloid widely used to treat cancer patients worldwide. However, the biosynthetic pathway and transcriptional regulation of camptothecin are largely unknown. Ophiorrhiza pumila, the herbaceous plant from the Rubiaceae family, has emerged as a model plant for studying camptothecin biosynthesis and regulation. In this study, a high-quality reference genome of O. pumila with estimated size of ~456.90 Mb was reported, and the accumulation level of camptothecin in roots was higher than that in stems and leaves. Based on its spatial distribution in the plant, we examined gene functions and expression by combining genomics with transcriptomic analysis. Two loganic acid O-methyltransferase (OpLAMTs) were identified in strictosidine-producing plant O. pumila, and enzyme catalysis assays showed that OpLAMT1 and not OpLAMT2 could convert loganic acid into loganin. Further knock-out of OpLAMT1 expression led to the elimination of loganin and camptothecin accumulation in O. pumila hairy roots. Four key residues were identified in OpLAMT1 protein crucial for the catalytic activity of loganic acid to loganin. By co-expression network, we identified a NAC transcription factor, OpNAC1, as a candidate gene for regulating camptothecin biosynthesis. Transgenic hairy roots and biochemical assays demonstrated that OpNAC1 suppressed OpLAMT1 expression. Here, we reported on two camptothecin metabolic engineering strategies paving the road for industrial-scale production of camptothecin in CPT-producing plants.

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“…Presence of camptothecin and its derivatives has been isolated from various parts of P. volubilis such as cotyledons, ripened whole fruit, and root, followed by seed coat, fruit coat, and young and mature leaves using liquid chromatography and MS analysis [2]. Preliminary phytochemical screening reveals the presences of alkaloids, phenolic compounds, saponins, glycosides, carbohydrates, proteins, gums, mucilage, fixed oils, fats, and resins.…”

Section: Phytochemical Screeningmentioning

confidence: 99%

“…Pyrenacantha volubilis is a climber and member of Icacinaceae family. Literature study reveals that P. volubilis possess richest source of camptothecin and its derivatives that have been isolated from the cotyledons, ripened whole fruit, and root, followed by seed coat, fruit coat, and young and mature leaves [2]. In silico docking studies reveal that camptothecin possesses good binding energy with inflammatory mediators such as MAPK, NFĸB, tumor necrosis factor (TNF)-α, and cyclooxygenases 1 and 2 [3] and in vitro study results suggest that camptothecin inhibits angiogenesis, synoviocyte proliferation, and metalloproteinase expression [4].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anti-Inflammatory and Anti-Arthritic Activity of Ethanolic Extract of Leaves of Pyrenacantha Volubilis (Eepv)

Anjali¹,

Vimalavathini²,

Kavimani³

2020

Asian J Pharm Clin Res

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Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods. Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard. Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium. Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.

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Pyrenacantha volubilis Wight, (Icacinaceae) a rich source of camptothecine and its derivatives, from the Coromandel Coast forests of India

Cited by 19 publications

References 19 publications

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

OpNAC1 transcription factor regulates the biosynthesis of the anticancer drug camptothecin by targeting loganic acid O‐methyltransferase in Ophiorrhiza pumila

In Vitro Anti-Inflammatory and Anti-Arthritic Activity of Ethanolic Extract of Leaves of Pyrenacantha Volubilis (Eepv)

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