Pyrene degradation by Mycobacterium sp. strain KR2

Rehmann, Klaus; Noll, Harald; Steinberg, Christian; Kettrup, A.

doi:10.1016/s0045-6535(97)10240-5

Cited by 148 publications

(88 citation statements)

References 37 publications

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“…This is consistent with the mechanism by which the strain attacks fluoranthene (see below). It is also consistent with the reported 1,2-dioxygenation of pyrene and subsequent meta cleavage (Heitkamp et al, 1988a,b;Walter et al, 1991) and the 4, 5-dioxygenation followed by ortho cleavage (Rehmann et al 1998), both seen with Mycobacterium and Rhodococcus sp. that are able to grow on pyrene.…”

Section: Microbiological Studiessupporting

confidence: 91%

“…However, the Mycobacterium stains clearly have the ability to further metabolize the intermediates produced from a naphthalene-like attack on pyrene. This was verified by the work of Rehmann et al (1998) and Heitkamp et al (1988a,b), who observed further degradation products. Why Sphingomonas strains have not acquired the necessary enzymes for this transformation is unclear.…”

Section: Microbiological Studiessupporting

confidence: 66%

“…A number of studies, have shown that bacteria are able to grow on the four-ring PAHs, specifically, fluoranthene (Boldrin et al, 1993;Dagher et al, 1996;Kastner et al, 1994;LloydJones and Hunter, 1997;Mueller et al, 1990;Weissenfeis et al, 1991) and pyrene (Boldrin et al, 1993;Churchill et al, 1999;Dean-Ross and Cerniglia, 1996;Fritzsche, 1994;Grosser et al, 1991;Heitkamp et al, 1988a,b;Jimenez and Bartha, 1996;Kastner et al, 1994;Lloyd-Jones and Hunter, 1997;Rehmann et al, 1998;Schneider et al, 1996). Mycobacterium, Rhodococcus, Alcaligenes, and Sphingomonas are the genera commonly encountered.…”

Section: Microbiological Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Federal Integrated Biotreatment Research Consortium (FIBRC): Flask to Field Initiative

Bajpai¹,

Felt²,

Nestler³

et al. 2002

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Section: Microbiological Studiessupporting

confidence: 91%

Section: Microbiological Studiessupporting

confidence: 66%

Section: Microbiological Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Federal Integrated Biotreatment Research Consortium (FIBRC): Flask to Field Initiative

Bajpai¹,

Felt²,

Nestler³

et al. 2002

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“…5,6-Benzocoumarin was reported earlier as one of the compounds produced from phenanthrene-1,2-dihydrodiol (Pinyakong et al, 2000). Although the intradiol cleavage of highmolecular-mass PAH diols is usually limited to K-region diols (Rehmann et al, 1998;Vila et al, 2001), there are reports on intradiol cleavage of the non-K region diol of PAHs (Dean-Ross et al, 2001;Rehmann et al, 2001;Keum et al, 2006;Kim et al, 2005;Lopez et al, 2006). Naphthalene-1,2-dicarboxylic acid identified in this study was found to be a dead-end metabolite and was not degraded further by strain PN/Y.…”

Section: Discussionmentioning

confidence: 99%

A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2,3-dioxo-5-(2′-hydroxyphenyl)-pent-4-enoic acid

2007

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Staphylococcus sp. strain PN/Y, capable of utilizing phenanthrene as a sole source of carbon and energy, was isolated from petroleum-contaminated soil. In the degradation of phenanthrene by strain PN/Y, various metabolites, isolated and identified by a combination of chromatographic and spectrometric analyses, revealed a novel phenanthrene assimilation pathway involving 2-hydroxy-1-naphthoic acid. Metabolism of phenanthrene was initiated by the dioxygenation on the 1,2-position of phenanthrene followed by meta-cleavage of phenanthrene-1,2-diol, leading to 2-hydroxy-1-naphthoic acid, which was then processed via a novel meta-cleavage pathway, leading to the formation of trans-2,3-dioxo-5-(29-hydroxyphenyl)-pent-4-enoic acid and subsequently to salicylic acid. In the lower pathway, salicylic acid was transformed to catechol, which was then metabolized by catechol-2,3-dioxygenase to 2-hydroxymuconaldehyde acid, ultimately forming TCA cycle intermediates. The catabolic genes involved in phenanthrene degradation were found to be plasmid-encoded. This detailed study of polycyclic aromatic hydrocarbon (PAH) metabolism by a Gram-positive species involving a unique ring-cleavage dioxygenase in a novel phenanthrene degradation pathway provides a new insight into the microbial degradation of PAHs. INTRODUCTIONPolycyclic aromatic hydrocarbons (PAHs) constitute a group of priority environmental pollutants, which are ubiquitous contaminants in soils and sediments and are of environmental concern because of their toxic, mutagenic and/or carcinogenic effects (Mastrangelo et al., 1996;Marston et al., 2001;Xue & Warshawsky, 2005). In recent years, the biodegradation of PAHs has received considerable attention and a variety of micro-organisms have been reported to play important roles in the process (Pothuluri & Cerniglia, 1994;Shuttleworth & Cerniglia, 1995;Kanaly & Harayama, 2000;Habe & Omori, 2003;Tortella et al., 2005). Bioremediation technologies have increasingly been proposed to decontaminate PAH-contaminated sites (Harayama, 1997;Samanta et al., 2002;Parrish et al., 2004;Vinas et al., 2005).Phenanthrene, a PAH with three condensed rings fused in angular fashion, has a 'bay-region' and a 'K-region' and is often used as a model substrate for studies on the metabolism of carcinogenic PAHs. Over the last 60 years, a number of studies on phenanthrene degradation by several Gram-negative and Gram-positive bacterial species have been reported (Evans et al., 1965;Kiyohara et al., 1976Kiyohara et al., , 1982Kiyohara & Nagao, 1978;Barnsley, 1983;Gibson & Subramanian, 1984;Houghton & Shanley, 1994;Adachi et al., 1999;Samanta et al., 1999), where various pathways and metabolic diversity involved in phenanthrene degradation were documented. In general, the metabolic pathway is initiated by the double hydroxylation of the bay-region of phenanthrene by a dioxygenase enzyme to form cis-3,4-phenanthrenedihydrodiol. The resultant dihydrodiol is then converted by the action of dihydrodiol dehydrogenase to 3,4-dihydroxyphenanthrene, which und...

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“…Microbial degradation of PAHs is considered the major avenue of removal from the environment (Cerniglia, 1992), and members of the genus Mycobacterium have been shown to be degraders of PAHs with larger number of aromatic rings, such as fluoranthene (Bogan et al, 2003;Lopez et al, 2006), pyrene (Heitkamp et al, 1988;Rehmann et al, 1998) and benzo [a]pyrene (Moody et al, 2004;Schneider et al, 1996). Isolation of novel organisms remains interesting because of the potential for degradation of diverse substrates and/or for the presence of novel catabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils: Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov. and Mycobacterium aromaticivorans sp. nov.

Hennessee

Seo

Alvarez

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

105

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Pyrene degradation by Mycobacterium sp. strain KR2

Cited by 148 publications

References 37 publications

Federal Integrated Biotreatment Research Consortium (FIBRC): Flask to Field Initiative

Federal Integrated Biotreatment Research Consortium (FIBRC): Flask to Field Initiative

A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2,3-dioxo-5-(2′-hydroxyphenyl)-pent-4-enoic acid

Polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils: Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov. and Mycobacterium aromaticivorans sp. nov.

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