Pyrene-degradation potentials of Pseudomonas species isolated from polluted tropical soils

Obayori, Oluwafemi S.; Ilori, Matthew O.; Adebusoye, Sunday A.; Oyetibo, Ganiyu Oladunjoye; Amund, Olukayode O.

doi:10.1007/s11274-008-9790-7

Cited by 68 publications

(33 citation statements)

References 39 publications

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“…Also, it was noticed that the addition of 20 mg g -1 soil of plant-derived materials to soil resulted in the significantly (P0.05) higher increase in THB and crude oildegraders counts in Conocarpus-and Tamarix-amended soils compared to that when 60 and 100 mg g -1 soil of plant-derived materials were added to soil. These findings were in agreement with other studies (Al-Saleh and Obuekwe [2]; Obayori et al [11]). The identification of the crude oil-degrading bacteria revealed the predominance of Gram-negative crude oil-degrading bacteria in the unamended and amended contaminated soil samples which might be due to the higher ability of Gram-negative bacteria to adapt to crude oil pollution.…”

Section: Discussionsupporting

confidence: 94%

Characterization of crude oil-degrading bacteria in a crude oil-contaminated and uncontaminated site in Kuwait

Hassan¹,

Taqi²,

Obuekwe³

et al. 2011

Sustainability Today

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The planning of cities must insure a balance between human consumption and natural resources that should be preserved and if needed should be used minimally and efficiently following plans of sustainability. The current study investigated the possibility of recycling natural waste product "plants leaves" that usually fall off, are collected, dumped and burnt. In counties like Kuwait where crude oil constitutes a major pollutant and the environment is hostile for microbial activity rendering bioremediation to be a trivial option. Thus, in the present study, the leaves of Conocarpus and Tamarix were used to enrich the low organic content soils that showed the potential of soil supplementation with organic matter to enhance the growth and activity of soil indigenous microbiota. The amendments of soils with 20 mg g -1 soil significantly increased the counts of crude oil-degrading bacteria in crude oil-contaminated and uncontaminated soils to 999.4 x 10 -3 CFU g -1 and 358.8 x 10 -3 CFU g -1 soil, respectively. The identification of isolated bacteria revealed the dominance of the genus Microbacterium (39.6%), Sphingopyxis soli (19.3%), and Bordetella petrii (19.6%) in unamended, Conocarpus-amended and Tamarix-amended contaminated soils, respectively. The 16S rRNA analyses showed the high diversity of isolated bacteria. Also, the diversity of the majority of isolated bacteria decreased after soil amendments with plant-derived material. Therefore, the recycling of plant-derived materials could be an excellent option under conditions tested and confirm the commandments of sustainable planning.

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Section: Discussionsupporting

confidence: 94%

Characterization of crude oil-degrading bacteria in a crude oil-contaminated and uncontaminated site in Kuwait

Hassan¹,

Taqi²,

Obuekwe³

et al. 2011

Sustainability Today

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“…Based on these characteristics, it was putatively identified as Bacillus amyloliquefaciens. It is noteworthy that though, previous reports have established the propensity of PAHs degraders from the Nigerian environment (Ilori and Amund, 2000;Igwo-Ezikpe et al, 2006;Obayori et al, 2008;Salam et al, 2014), to the best of our knowledge, this is the first report of fluorene degraders from the Nigerian environment. The two isolates tolerated NaCl concentration of 7% with good and moderate growth exhibited by strain BR1 and BM1, respectively.…”

Section: Resultsmentioning

confidence: 62%

“…This physiological property favours the use of these strains as possible candidates for bioaugmentation purpose. Previous reports have shown that salinity could be a critical factor that determines the survival of allochthonous bacterial strains during bioremediation (Kästner et al, 1998;Obayori et al, 2008). In addition, the two strains resisted ceftriazone and cotrimozaxole but were susceptible to ofloxacin, gentamicin, and ciprofloxacin.…”

Section: Resultsmentioning

confidence: 91%

“…Only strain BM1 was resistant to both amoxycilin and streptomycin. Shared resistance of strains BM1 and BR1 to ceftriazone and cotrimozaxole may be attributed to acquisition of resistant genes to these antibiotics through gene transfer, as soil environments are replete with these antibiotics, which could allow evolution of resistance by indigenous strains (Obayori et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

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Fluorene biodegradation potentials of Bacillus strains isolated from tropical hydrocarbon-contaminated soils

Salam¹,

Obayori²

2014

Afr. J. Biotechnol.

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Two fluorene-degrading Gram-positive Bacillus strains, putatively identified as Bacillus subtilis BM1 and Bacillus amyloliquefaciens BR1 were isolated from hydrocarbon-and asphalt-contaminated soils in Lagos, Nigeria. The polluted soils have a relatively high total hydrocarbon content (16888.9 and 9923.1 mg/kg, respectively), very low concentrations of macronutrients and the total organic carbon was less than 4%. The two strains tolerated NaCl concentration of up to 7% while strain BR1 exhibited moderate growth at 10%. Shared resistance to ceftriazone and cotrimozaxole were exhibited by both strains while only strain BM1 was resistant to both amoxycilin and streptomycin. The rate of degradation of fluorene (50 mg/L) by the two isolates, after 30 days of incubation were 0.09 and 0.08 mg/L/h for strains BM1 and BR1, respectively. Gas chromatographic analyses of residual fluorene, revealed that 56.9 and 46.8% of 50 mg/L fluorene was degraded in 12 days by strains BM1 and BR1. However, after 21 days on incubation, 86 and 82% of 50 mg/L fluorene were degraded by strains BM1 and BR1, respectively. To the best of our knowledge, this is the first report highlighting flourene degradation potential of Bacillus strains isolated from tropical African environment.

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“…In relation to the growth rate, the levels are much higher than those reported by Obayori et al (2008) when they studied PYR biodegradation by a Pseudomonas species isolated from polluted tropical soils (0.024 h -1 ). It is noticeable that the introduction of a mixture of the three PAHs leads to a decrease both in the maximum biomass levels and growth rate.…”

Section: Growth and Biodegradation Of Pahs In Cultures At Flask Scalementioning

confidence: 63%

Insights into polyaromatic hydrocarbon biodegradation by Pseudomonas stutzeri CECT 930: operation at bioreactor scale and metabolic pathways

Moscoso

Deive

Longo

et al. 2014

Int. J. Environ. Sci. Technol.

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Contamination of the environment with polycyclic aromatic hydrocarbons is one of the major problems facing the industrialized nations today. In this work, Pseudomonas stutzeri CET 930 was studied for the first time as bioremediation agent for the degradation of effluents containing phenanthrene, pyrene and benzanthracene, both individually and mixed. The promising results of degradation obtained at flask scale (92, 63 and 94 % in 7 days, respectively) marks the onset of the operation at bench scale bioreactor. The overall biotransformation of phenanthrene, pyrene and benzanthracene in batch operation mode was 95, 78 and 82 % when present individually, and 100, 98 and 100 % when carrying out the biological process in cometabolic conditions, respectively. We have demonstrated the great versatility of this strain for the degradation of structurally different contaminants, such as metal working fluids, polycyclic aromatic hydrocarbons or insecticides such as chlorpyrifos, which makes it a suitable candidate to be applied at industrial scale. In all cases, the experimental data were successfully fitted to models, which turned out to be valuable tools to classify the metabolites involved in the biodegradation process as biomass-related. Since a complete mineralization was pursued, the metabolic pathways of the studied contaminants have been proposed based on gas chromatography-mass spectrometry data.

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Pyrene-degradation potentials of Pseudomonas species isolated from polluted tropical soils

Cited by 68 publications

References 39 publications

Characterization of crude oil-degrading bacteria in a crude oil-contaminated and uncontaminated site in Kuwait

Characterization of crude oil-degrading bacteria in a crude oil-contaminated and uncontaminated site in Kuwait

Fluorene biodegradation potentials of Bacillus strains isolated from tropical hydrocarbon-contaminated soils

Insights into polyaromatic hydrocarbon biodegradation by Pseudomonas stutzeri CECT 930: operation at bioreactor scale and metabolic pathways

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