Pyrene Sulphonyl Chloride as a Reagent for Quantitation of Oestrogens in Human Serum Using HPLC with Conventional and Laser-Induced Fluorescence Detection

3-(Dichloro-1,3,5-triazinyl)benz[f]isoindolo[1,2-b][1,3]thiazolidine as a Derivatization Reagent for Bisphenols in High-Performance Liquid Chromatography with Argon Laser-induced Fluorescence Detection

“…This did not result in the formation of the corresponding DTBIT derivative. Recently, a simple and rapid fluorometric derivatization procedure for estrogens with PSCL was reported, 6 based on a two-phase system with TPABr used as a phase-transfer catalyst. Under the reaction conditions mentioned in the literature, the phenolic compounds present in the organic phase are deprotonated at the interface by means of the NaOH present in the aqueous phase.…”

“…1-ethoxy-4-(dichloro-s-triazinyl)naphthalene, 2 4-(N,N-dimethylamino)-naphthalene-1-carbonitrile, 3 1-anthronitrile, 4 9-anthronitrile, 4 pyrene-1-carbonitrile, 5 1-pyrenesulfonyl chloride (PSCL), 6 4-(N-phthalimidinyl)benzenesulfonyl chloride 7 and 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole. 8 However, these reagents have not been available to the HPLC determination of bisphenols as environmental chemicals with endocrinedisrupting activity, because of a lack of sensitivity.…”

Hyphenated chromatographic methods for biomaterials

“…However, the choice of excitation wavelength, which is limited in LIF, represents the main disadvantage of this technique. HPLC-LIF was applied for determining some hormones that are known to be at very low concentrations (Okumura et al, 1995;DeSilva et al, 1996). Salivary cortisol as a stress marker was determined using Ar-ion LIF at 488 nm.…”

“…An LOD of 8 fmol per 400 mL injection with a simple sample pretreatment (ie dilution and filtration) of saliva was reported (Okumura et al, 1995). DeSilva et al (1996) determined b-estradiol in serum as a derivative of pyrenesulphonyl chloride. The on-column LOD obtained using the 325 nm line of He-Cd laser was 200 amol.…”

HPLC Determination of Acetaminophen in Saliva Based on Precolumn Fluorescence Derivatization with 12-(3,5-Dichloro-2,4,6-triazinyl)-benzo[d]benzo[1′,2′-6,5]isoindolo[1,2-b][1,3]thiazolidine

“…A large number of fluorescence derivatization reagents for the determination of phenolic hydroxyl groups in HPLC have been reported: e.g., 5-dimethylaminonaphthalene-1-sulfonyl chloride, 10 1-ethoxy-4-(dichloro-s-triazinyl)naphthalene, 11 4-dimethylaminonaphthalene-1-carbonitrile, 12 1-anthronitrile, 13 9-anthronitrile, 13 pyrene-1-carbonitrile, 14 1-pyrenesulfonyl chloride, 15 4-(N-phthalimidinyl)benzenesulfonyl chloride, 16 and 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole. 17 However, no data have been reported for the determination of APAP.…”