Mango (Mangiferae indica L.) is an important export horticultural crop of India. Various insects and diseases infest the fruit especially in the fruiting season. The common insect pests are mango leafhopper, mealy bug, leaf webber, inflorescence midge and fruit fly. The major loss of about 60% is due to leafhopper and mango leafhopper. These pests infest the mango at the floral and bud stage to fruit setting, tender leaves and small fruits. The crop protection measures undertaken to control pest infestation involves spray of insecticides along with biological and cultural practices.India produces 65% of the world's mango crop, 9,000,000 MT, but with very little export. Although, Asia accounts for 75% world production, its dominance does not translate into international trade. The presence of pesticide remnants in mango lowers the export quality of mango fruits in the international market. To increase foreign trade, under the WTO regime, it is imperative to produce pesticide free mangoes.The insecticides commonly applied by the farmers are endosulfan, parathion methyl, chlorpyrifos, cypermethrin and fenvalerate. This paper presents a method for the estimation of the multi-class pesticides in mango and their recovery.
Materials and MethodSolvents like acetone, dichloromethane, hexane (analytical grade), were distilled before use. Adsorbents neutral alumina and Florisil were activated before use. Pesticide standards of methyl parathion, endosulfan, chlorpyrifos, cypermethrin and fenvalerate were of analytical grade quality. Gas Chromatogram instrumen-Shimadzu GC-17A fitted with an auto-sampler and ECD detector was used for analysis. Other minor equipments required were rotary evaporator and Waring blender, etc.Individual stock standard solutions (1,000 lg/mL) of parathion methyl, endosulfan, chlorpyrifos, cypermethrin and fenvalerate were prepared in hexane (analytical grade). Working standard solutions of each pesticide was prepared by serial dilutions to 100 lg/mL, which was further diluted to 0.1-10 lg/mL as per detector response. All the stock solutions were stored at 4°C. Appropriate aliquot of individual pesticides solutions were taken and a mixture of five pesticides of 100 lg mL -1 concentration were prepared. Mixture solutions of lower concentrations were prepared by serial dilutions using hexane.Limit of detection (LOD) of each pesticide, individually and in a mixture was determined by injecting standard solutions of different concentration levels in duplicate in the GLC. The lowest concentration of the pesticide that gave peak area five times that of background level was considered as LOD.Mango fruit samples without peel (20 g, cut into small pieces) were fortified with the standard mixture at 0.5 lg/mL levels in ten sets each set in triplicate. One control was set-aside for each of the sets. The spiked mango samples were extracted in a Waring blender-Remi make with acetone 50 ml for 2-3 min. The solvent was