Pyridoxine Administration Improves Behavioral and Anatomical Outcome after Unilateral Contusion Injury in the Rat

Abstract

The purpose of this project was to evaluate the preclinical efficacy of pyridoxine, or vitamin B 6 . Rats received a 3.0 mm unilateral controlled cortical impact (CCI) injury of the sensorimotor cortex or sham surgery. Treatment with vitamin B 6 (600 or 300 mg/kg IP) or vehicle was administered at 30 min and 24 h post-CCI. Somatosensory dysfunction was evaluated with the vibrissae-forelimb placing and bilateral tactile adhesive removal tests. Sensorimotor dysfunction was evaluated with the locomotor placing and the forelimb asymmetry tests. On the forelimb asymmetry test both treatment groups displayed no asymmetry bias on any of the testing days post-CCI and were statistically no different than the shams. Both vitamin B 6 groups displayed a significant improvement in behavioral performance on the locomotor placing test compared to the vehicle-treated group. Administration of 600 mg/kg also significantly reduced tactile adhesive removal latencies on days 2, 4, 6, and 12 post-CCI. Both treatment groups were improved in their rate of recovery post-CCI on the vibrissae-forelimb placing test, but only the recovery seen in the 600-mg/kg group was significantly improved compared to vehicle. Finally, the 600-mg/kg dose resulted in significant cortical sparing compared to the vehicle-treated group. In general, the effects of vitamin B 6 on recovery of function were dose-dependent, with the 600-mg/kg dose consistently showing greater recovery than the 300-mg/kg dose. More experimental analyses are warranted to evaluate the potential preclinical efficacy and mechanistic action of vitamin B 6 .

Shedding new light on neurodegenerative diseases through the mammalian target of rapamycin

“…Once generated, ROS alter mitochondrial function, DNA integrity, and the misfolding of proteins leading to cellular injury and the progression of aging mechanisms (Jayaram et al , 2011; Maiese et al , 2010; Yang et al , 2011). Detrimental effects of ROS are usually prevented by endogenous antioxidant systems that include superoxide dismutase, glutathione peroxidase, catalase, and vitamins that include C, D, E, and K (Chong et al , 2005e; Goffus et al , 2010; Herbas et al , 2011; Kuypers and Hoane, 2010; Lappas and Permezel, 2011; Maiese and Chong, 2003; Maiese et al , 2009b; Suzen et al , 2012; Vonder Haar et al , 2011; Wang et al , 2012b; Yuan et al , 2012). During exposure to oxidative stress, mTOR pathways can become depressed and lead to cell injury (Andreucci et al , 2009; Chen et al , 2010; Shang et al , 2011).…”

A behavioral and histological comparison of fluid percussion injury and controlled cortical impact injury to the rat sensorimotor cortex

“…[19, 28, 29] The forelimb SMC is well known for the plastic responses that follow both lesions and ischemic insult. [for review, see: 30, 31, 32] To summarize briefly, behavioral deficits ensue in the injured forelimb (contralateral to injury) following lesions of the forelimb SMC.…”

Experimental immunology The effect of vitamin B 6 supplementation of protein deficiency diet on hematological parameters in the blood of rats subjected/non subjected to physical exertion – a pilot study

“…A diet DOI: 10.5114/ceji.2012.30791 enriched in vitamin B 6 prevents alcohol-induced pressure and reduces blood pressure in spontaneously hypertensive rats [11,12]. In addition, an increased dose of vitamin B 6 reduces the recovery period in rats with induced injuries [13]. Therefore, in this study we decided to determinate the effect of vitamin B 6 on hematological parameters of rats fed a protein deficiency diet with or without physical exertion.…”