Pyriproxyfen influences growth as well as thyroid hormone–related and gh/igf-1 gene expression during the early life stage of zebrafish (Danio rerio)

Horie, Yoshinori; Mitsunaga, Kensuke; Yap, Chee Kong

doi:10.1016/j.cbpc.2023.109632

Cited by 5 publications

(2 citation statements)

References 50 publications

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“…Therefore, as indicated above, it appears that the decrease in body weight is a common impact of PPF. PPF has been shown to disrupt thyroid hormone activity in zebrafish and amphibians during their early stages of development [27,32] . The thyroid hormones have been documented to be one of the factors controlling muscle mass, and their alterations may potentially contribute to the development of muscular atrophy [33] .…”

Section: Discussionmentioning

confidence: 99%

“…This was followed by limb length of PPF low dose on ED14, which displayed a significant difference in humerus length of forelimbs and metatarsus of hindlimbs. According to Horie et al [32] , this could be attributable to pyriproxyfen's inhibitory action on growth hormone. As previously stated, growth hormone deficiency severely restricts both bone growth and remodeling processes [35] .…”

Section: Discussionmentioning

confidence: 99%

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The Teratogenic Effects of the Insecticide Pyriproxyfen on the Developing Chick Embryos

Sedeek,

Hafez,

Mohallal

et al. 2023

Egyptian Journal of Zoology

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Pyriproxyfen (PPF) is a juvenile hormone analogue insecticide that is often used as a larvicide against a wide range of insect pests. The present study was designed to investigate its teratogenic effects on the embryonic development of the chick. First, the median lethal dose (LD50) of the commercial PPF was determined. Accordingly, three sub-lethal doses (15, 30, 45 µg/egg) were selected for injection into the air space of the eggs after 24 hours of incubation. The eggs were opened on the embryonic days (EDs) 7 and 14, and the embryos were examined for morphometric changes and the presence of malformations. The PPF treatment induced growth retardation and reduction in head and eye sizes as reflected by a reduction in wet body weights, crown-rump lengths, anterior-posterior head lengths, and eye diameters. These morphometric alternations were mild or moderate on ED7, but more apparent on ED14. Also, significant reductions in the lengths of the forelimb and hindlimb parts were recorded with high-dose treatment on ED14. Obviously, there was high percentage of malformations among ED7 individuals in the form of hematoma, ventral body wall defect, limb deformities, microphthalmia, and microcephaly. However, on ED14 the embryos exhibited significant hematoma, microcephaly, delay feather, delay beak, microphthalmia in all treated groups, and limb deformities in the group that received the high dose. Other abnormalities included edema, anophthalmia, short neck, short tail, caudal regression, microtia, and microblepharon were also recorded. These findings revealed that PPF has potentially teratogenic effects on the development of the chick embryos.Pyriproxyfen-induced teratogenicity in chick embryos

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Teratogenic Effects of the Insecticide Pyriproxyfen on the Developing Chick Embryos

Sedeek,

Hafez,

Mohallal

et al. 2023

Egyptian Journal of Zoology

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Female Mice Exposed to Pyriproxyfen Since Prepuberty Showed Reproductive Impairment During Sexual Maturity and Increased Fetal Death in Their Offspring

da Silva,

de Mello,

Fagá

et al. 2024

Environmental Toxicology

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Pyriproxyfen (PPF) is an insecticide used in agriculture, which is approved for use in drinking water tanks for human consumption. However, some studies indicate that it may act as an endocrine disruptor and affect nontarget organisms. This study aimed to evaluate the effects of PPF on reproduction and general health status in female mice exposed from pre‐puberty to adulthood. In the first experiment, females were treated by gavage from postnatal day (PND) 23 to (PND) 75 and were distributed into three experimental groups: control (vehicle), PPF 0.1 mg/kg, and PPF 1 mg/kg. Female mice were assessed for the age of puberty onset, body mass, water and food consumption, and the estrous cycle. On PDN 75, a subgroup was euthanized, when vital and reproductive organs were collected and weighed. The thyroid, ovary, and uterus were evaluated for histomorphometry. The other subgroup was assessed in relation to reproductive performance and fetal parameters. In a second experiment, the uterotrophic assay was performed with juvenile females (PND 18) using doses of 0.01, 0.1, or 1 mg/kg of PPF. PPF treatment reduced thyroid mass and increased liver mass. Furthermore, there was an increase in ovarian interstitial tissue and, in the uterus, a decrease in the thickness of the endometrial stroma with reduced content of collagen fibers. There was also a reduction of 30% in pregnancy rate in the treated groups and an increase in the frequency of fetal death. This study suggests that, based on this experimental model, the insecticide may pose a reproductive risk for females chronically exposed to the substance from the pre‐pubertal period until adulthood. These results raise concerns about prolonged exposure of women to the same compound.

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Exposure to Pyriproxyfen Impacts Heart Development Causing Tissue and Cellular Impairments, Heart Arrhythmia and Reduced Embryonic Growth

Bernhardt,

Ronconi-Krüger,

Nazari

2024

Cardiovasc Toxicol

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Pyriproxyfen influences growth as well as thyroid hormone–related and gh/igf-1 gene expression during the early life stage of zebrafish (Danio rerio)

Cited by 5 publications

References 50 publications

The Teratogenic Effects of the Insecticide Pyriproxyfen on the Developing Chick Embryos

The Teratogenic Effects of the Insecticide Pyriproxyfen on the Developing Chick Embryos

Female Mice Exposed to Pyriproxyfen Since Prepuberty Showed Reproductive Impairment During Sexual Maturity and Increased Fetal Death in Their Offspring

Exposure to Pyriproxyfen Impacts Heart Development Causing Tissue and Cellular Impairments, Heart Arrhythmia and Reduced Embryonic Growth

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