Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum

Völkl, Paul; Huber, Robert; Drobner, Elisabeth; Rachel, Reinhard; Burggraf, Siegfried; Trincone, Antonio; Stetter, Karl O.

doi:10.1128/aem.59.9.2918-2926.1993

Cited by 277 publications

(106 citation statements)

References 33 publications

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“…The biochemical characterization of the recombinant P. aerophilum ACS revealed several unique molecular and catalytic properties: (i) The enzyme has temperature optimum above 97°C and the high thermostability up to 100°C, which is in accordance with its function at the optimal growth temperature of P. aerophilum of 100°C [24]. (ii) The hyperthermophilic ACS is a 610 kDa homooctamer composed of 75 kDa subunits (calculated 76.2 kDa).…”

Section: Discussionmentioning

confidence: 96%

A novel octameric AMP‐forming acetyl‐CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

2004

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AMP-forming acetyl-CoA synthetases (ACSs) are ubiquitous in all three domains of life. Here, we report the first characterization of an ACS from a hyperthermophilic organism, from the archaeon Pyrobaculum aerophilum. The recombinant ACS, the gene product of ORF PAE2867, showed extremely high thermostability and thermoactivity at temperatures around 100°C. In contrast to known monomeric or homodimeric mesophilic ACSs, the P. aerophilum ACS was a 610 kDa homooctameric protein, with a significant lower content of thermolabile (Cys, Asn, and Gln) and higher content of charged (Glu, Lys, and Arg) amino acids. Kinetic analyses revealed an unusual broad substrate spectrum for organic acids and an extremely high affinity for acetate (K m 3 lM).

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Section: Discussionmentioning

confidence: 96%

A novel octameric AMP‐forming acetyl‐CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

2004

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“…the hyperthermophilic crenarchaeon Pyrobaculum sp. (Volkl et al, 1993) or the halophilic euryarchaeon Halogeometricum (Cui et al, 2010). The nirB gene was detected in Thermococcus sp.…”

Section: Nitrite Reductionmentioning

confidence: 99%

Genomic evidence for distinct carbon substrate preferences and ecological niches of Bathyarchaeota in estuarine sediments

Lazar

Baker²,

Seitz³

et al. 2016

Environmental Microbiology

219

195

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Investigations of the biogeochemical roles of benthic Archaea in marine sediments are hampered by the scarcity of cultured representatives. In order to determine their metabolic capacity, we reconstructed the genomic content of four widespread uncultured benthic Archaea recovered from estuary sediments at 48% to 95% completeness. Four genomic bins were found to belong to different subgroups of the former Miscellaneous Crenarcheota Group (MCG) now called Bathyarchaeota: MCG-6, MCG-1, MCG-7/17 and MCG-15. Metabolic predictions based on gene content of the different genome bins indicate that subgroup 6 has the ability to hydrolyse extracellular plant-derived carbohydrates, and that all four subgroups can degrade detrital proteins. Genes encoding enzymes involved in acetate production as well as in the reductive acetyl-CoA pathway were detected in all four genomes inferring that these Archaea are organo-heterotrophic and autotrophic acetogens. Genes involved in nitrite reduction were detected in all Bathyarchaeota subgroups and indicate a potential for dissimilatory nitrite reduction to ammonium. Comparing the genome content of the different Bathyarchaeota subgroups indicated preferences for distinct types of carbohydrate substrates and implicitly, for different niches within the sedimentary environment.

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“…Despite the very interesting characteristics of thermal waters, the bacterial diversity of the Ischia system has never been studied. A few studies (Völkl et al, 1993;Schiano Morello et al, 2003) were focused on the isolation of some strains from sea sand at Maronti beach (southern Ischia).…”

Section: Introductionmentioning

confidence: 99%

Potential use of microbial community investigations to analyse hydrothermal systems behaviour: the case of Ischia Island, Southern Italy

Bucci

Naclerio

Allocca

et al. 2010

Hydrological Processes

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Abstract:The Ischia hydrothermal system was analysed through hydrogeological and microbial community investigations. Mesophilic communities were detected in two cold springs, suggesting a negligible influence of thermal circuits in freshwater sub-systems which are mainly or only fed by local precipitations. Thermophilic and extremely thermophilic bacteria were detected in two wells, according to higher water temperatures (61 and 85°C), even if the two communities show significant differences. In one well, thermophilic and extremely thermophilic bacteria are associated with strains belonging to ε-Proteobacteria isolated in different sulphur-rich carbonate environments. This association suggests a greater influence on ascending hot fluids that interact with the carbonate basement of volcanic rocks. In the other well, thermophilic and extremely thermophilic bacteria are associated with strains isolated in cold hypersaline environments or in aquatic habitats where terrestrial and marine components are coupled. This association supports the fact that seawater intrusion can affect this part of Ischia, according to results of hydrogeological and geochemical surveys. Differences in groundwater temperature and bacterial communities are probably mainly due to differences in permeability between volcanic rocks and differences in hydrogeologic behaviour between faults in the upper carbonate basement, above the deep magma chamber, that influence relationships between ascending hot fluids and local recharge. This study contributes to discussion of the reliability of the actual behaviour models of the Ischia system, based on the results of geochemical and isotopic investigations, and, in a wider context, it shows that microbial community investigations may be a valuable supplementary tool for analysing hydrothermal system behaviour.

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Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum

Cited by 277 publications

References 33 publications

A novel octameric AMP‐forming acetyl‐CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

A novel octameric AMP‐forming acetyl‐CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

Genomic evidence for distinct carbon substrate preferences and ecological niches of Bathyarchaeota in estuarine sediments

Potential use of microbial community investigations to analyse hydrothermal systems behaviour: the case of Ischia Island, Southern Italy

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