Pyrolysis Gas-Liquid Chromatography Studies of the Genus Bacillus. Effect of Growth Time on Pyrochromatogram Reproducibility

Oxborrow, Gordon S.; Fields, N. D.; Puleo, J. R.

doi:10.1016/b978-0-444-41558-5.50011-0

Cited by 14 publications

(4 citation statements)

References 13 publications

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“…These differences are assumed to reflect variations in the growth rate and metabolic activity of the three bacteria. Similar observations for culture age have been made with other organisms (2,13,16,19), but, to our knowledge, incubation temperature has not been considered.…”

Section: Discussionsupporting

confidence: 76%

Effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography

Gutteridge¹,

Norris²

1980

Appl Environ Microbiol

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High-resolution pyrolysis gas-liquid chromatography was applied to three bacteria (Escherichia coli NCTC 9001, Pseudomonas putida NCIB 9494, and Staphylococcus aureus NCTC 8532) grown under a variety of conditions. Changing the culture medium drastically altered the quantitative aspects of the pyrograms of all three organisms, but the effects of culture time and incubation temperature were less severe. Mathematical analysis of the relative peak heights showed that four peaks could be used to discriminate the three bacteria however they were cultured.

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Section: Discussionsupporting

confidence: 76%

Effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography

Gutteridge¹,

Norris²

1980

Appl Environ Microbiol

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“…Since then, PGLC has been used in the differentiation of numerous types of bacteria (10, 18, 22) and fungi (5,231. The recent application of PGLC to aerobic sporeformers by Oxborrow et al (12)(13)(14) indicates that, providing the cultural and chromatographic conditions remain constant, PGLC can be applied usefully to the characterization of bacilli.The variation between pyrograms of the same strain and the high level of redundancy found in PGLC data require the application of data processing techniques capable of highlighting significant variations in the heights of specific peaks. Several methods for handling data in this t Present address: School of Chemistry, The University, Newcastle-upon-Tyne, NE1 7RU, United Kingdom.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens by Pyrolysis Gas-Liquid Chromatography, Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization, Biochemical Tests, and API Systems

O'Donnell

Norris

Berkeley

et al. 1980

International Journal of Systematic Bacteriology

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Eight strains each of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens were analyzed by using pyrolysis gas-liquid chromatography. Statistical analysis with canonical variates gave four well-separated groups, which represented the four species. Further analysis of the same strains by deoxyribonucleic acid-deoxyribonucleic acid hybridization and API identification systems confirmed the discrimination obtained with pyrolysis gasliquid chromatography. However, analysis by biochemical tests performed in the classical way gave only three groups since it was not possible to achieve separation of the strains representing B. subtilis from those of B. amyloliquefaciens when these tests were used.Pyrolysis, a process whereby molecules are thermally degraded in an inert gas atmosphere, has enhanced the use of conventional gas-liquid chromatography by enabling nonvolatile compounds to be analyzed. Pyrolysis gas-liquid chromatography (PGLC) was fmt proposed as an approach to microbial differentiation by Oyama (15) during the development of a system aimed at detecting life on Mars. However, its potential in microbiology was not appreciated until Reiner (17) was able to distinguish different species of Mycobacterium and different serotypes of Escherichia coti in a reproducible manner. Since then, PGLC has been used in the differentiation of numerous types of bacteria (10, 18, 22) and fungi (5,231. The recent application of PGLC to aerobic sporeformers by Oxborrow et al. (12)(13)(14) indicates that, providing the cultural and chromatographic conditions remain constant, PGLC can be applied usefully to the characterization of bacilli.The variation between pyrograms of the same strain and the high level of redundancy found in PGLC data require the application of data processing techniques capable of highlighting significant variations in the heights of specific peaks. Several methods for handling data in this t Present address: School of Chemistry, The University, Newcastle-upon-Tyne, NE1 7RU, United Kingdom.way have been described (10, 16), but as yet there is no agreement on the best statistical approach, and much work remains to be done in this field. This paper reports on the usefulness of lowresolution PGLC when coupled to multivariate data analysis for differentiating closely related groups of bacteria and provides evidence for the separation of Bacillus antyloliquefaciens from Bacillus subtilis. MATERIALS AND METHODS

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“…Because the data are generated by a standard procedure which is recorded automatically, the acquisition of a proportion of redundant information, similar to that found using physiological tests to differentiate organisms (8), is of minor importance. However, the parameters involved in this type of analysis re-quire careful evaluation before standardization and reproducibility can be made acceptable (13)(14)(15). Adequate treatment of the data is also necesary to obtain maximal information from the output (4,10).…”

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confidence: 99%

Discrimination between oral streptococci by pyrolysis gas-liquid chromatography

Stack¹,

Donoghue²,

Tyler³

1978

Appl Environ Microbiol

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Washed organisms, including strains of Streptococcus mitior, S. mutans, and S. sanguis, were examined by curie-point pyrolysis gas-liquid chromatography. A linear discriminant function based upon three items from the output data was adequate for segregating the strains according to species. Strains with intermediate properties were also encountered. Sources of variability in cultures were evaluated, chromatographic performance was maintained throughout the investigation, and matching performance from a duplicate pair of chromatographic columns was demonstrated.

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Pyrolysis Gas-Liquid Chromatography Studies of the Genus Bacillus. Effect of Growth Time on Pyrochromatogram Reproducibility

Cited by 14 publications

References 13 publications

Effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography

Effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography

Characterization of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens by Pyrolysis Gas-Liquid Chromatography, Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization, Biochemical Tests, and API Systems

Discrimination between oral streptococci by pyrolysis gas-liquid chromatography

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