Pyrolysis mass spectrometry of bacteria from infected human urine. I. Influence of culturing and antibiotics

Huff, Stanley M.; Matsen, John M.; Windig, Willem; Meuzelaar, Henk L. C.

doi:10.1002/bms.1200130604

Cited by 23 publications

(8 citation statements)

References 18 publications

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“…We have noted that the detection of intact phospholipids provides information on both the fatty acid distribution and the phospholipid content, the latter being generally less sensitive to bacterial growth conditions, and has the potential for providing greater specificity than gas chromatography/mass spectrometry analysis of the fatty acids alone. Bacterial cells are also analyzed directly by pyrolysis mass spectrometry (24,25). Pyrolysis mass spectra contain ions whose relationship to the structures of lipid biomarkers cannot be rationally established and bacteria are compared among themselves by factor analysis techniques (26).…”

Section: Discussionmentioning

confidence: 99%

Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardment

Heller¹,

Murphy²,

Cotter³

et al. 1988

Anal. Chem.

138

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Fast atom bombardment (FAB) of the lysed cells and crudellpld extracts of bacterla results In the selective desorption of phorphoupkk that are characteristic of bacterlal specks. I n thk paper, constant neutral loss (CNL) llnked scarmlng of the magnetk and electrlc sector flekls of a double focuslng mass spectrometer Is evaluated as an approach to the analysls of bacterla cells that can provlde greater selectlvlty for polar llplds and speclflclty for partlcular phosphollpld classes. I n the metastable t h e frame, neutral losses of phosphorylethanolamlne, phosphorylglycerol, and other polar head groups are be8t obtalned from protonated molecular Ions analyzed In the posltlve Ion mode. SelectlvHy Is Improved (relathre to normal magnetk fleld scans) for phosphollplds of a partlcular class In the presence of more abundant phosphollpMs and the background Ion dgnal characteristk of FAB m a s spectra. I n addklon, CNL scans penntl the observation of momnnethy@hoaphattklylethanolamhre, a mhror pbsphoUpkl prewnt In a few bacterla, such as P. vu&8f/s, but lndlstlngulshabk In mass from fatty acld homologues of PE contalnIng addltlonal methylene groups. Slnce fatty acld content In bacterial lipids (and therefore the masses appearlng In a particular spectrum) reflects growth condltlons as well as specks, the ldenttflcatlon of specllc phosphollpld classes by CNL scans provlcks a promlskrg approach to the klentllicatlon and cladlcatlon of bacterla.Membrane lipids are important biomarkers that can be used to differentiate bacteria from one another (1-3). We have found that desorption mass spectrometry techniques selectively ionize polar membrane lipids directly from lysed bacteria to provide mass spectra that are characteristic of Gram stain and species (4, 5). Both phospholipids and glycolipids are selectively desorbed by these techniques. The most common phospholipids contain two fatty acids, a glycerolphosphate core, and a polar head group which determines the phospholipid class (Scheme I). The variable portions of phospholipids (the head group and the fatty acids) are both found in distribution patterns that are chemotaxonomically significant.Thus, one approach to characterizing bacteria involves the chromatographic analysis of fatty acids extracted from bacteria cultured and harvested under standard conditions (6). Fatty acids are identified by retention times and their distribution is determined from the relative peak areas. While there are limits to the range of fatty acids that a given species will produce, the fatty acid distribution itself can vary significantly in the same species grown on different media, at different Scheme I -CO-O-CH, I CO-0-CH CH, I L/ 0-t-0 R J J R -CHiCH

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Section: Discussionmentioning

confidence: 99%

Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardment

Heller¹,

Murphy²,

Cotter³

et al. 1988

Anal. Chem.

138

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“…For example, PyMS studies on changes in bacterial cell composition during exposure to antimicrobial agents demonstrated the applicability of PyMS in rapidly detecting the effects of antimicrobial agents on microorganisms, and in studying the mode of action of antimicrobial agents. [74][75][76] Furthermore, in industry, PyMS with unsupervised analysis has been used for the selection of novel actinomycetes for pharmaceutical screening program, 77 while in environmental studies, the approach has been used to analyse rhizobial strains isolated from soil in an attempt to follow the spread of genetically engineered microbes.…”

Section: Other Microbiological Applicationsmentioning

confidence: 99%

Discrimination and Identification of Microorganisms by Pyrolysis Mass Spectrometry: From Burning Ambitions to Cooling Embers—A Historical Perspective

Timmins

Goodacre

2005

Identification of Microorganisms by Mass Spectrometry

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“…The mathematical formulation of the procedure described above is as follows: (22,26,29) it is known that DNA has a lower pyrolysis temperature than glycogen which, in turn, has a lower pyrolysis temperature than BSA (bovine serum albumin). Figure 1 shows the total ion current profile.…”

Section: Woodmentioning

confidence: 99%

Self-modeling curve resolution by factor analysis of a continuous series of pyrolysis mass spectra

Windig¹,

Jakab²,

Richards³

et al. 1987

Anal. Chem.

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Grade, H.; Winograd, N.; Cooks, R. G. J. Am. Chem. SOC. 1977, 99, . . --. . --. Sichtermann, W.; Benninghoven, A. Int . J. Mass Spectrom . Ion Phys. 1981, 40, 177-184. Liu, L. K.; Busch. K. L.; Cooks, R. G. Anal. Chem. 1981, 53, 109-1 13. Alai, M.; Demirev, P.; Fenselau, C.; Cotter, R. J. Anal. Chem. 1888, Lange, W.; Jirikowsky, M.; Benninghoven, A. Surf. Scl. 1984, 138, 419-436. Johnson, G. P.; Hedin, A. B.; Hakansson, P. L.; Sundqvist, B. U. R.; Save, B. G. S.; Nielson, P. F.; Roepstorff, P.; Johansson, K. E.; Kamensky, I.; Lindberg, M. S. L.

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Pyrolysis mass spectrometry of bacteria from infected human urine. I. Influence of culturing and antibiotics

Cited by 23 publications

References 18 publications

Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardment

Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardment

Discrimination and Identification of Microorganisms by Pyrolysis Mass Spectrometry: From Burning Ambitions to Cooling Embers—A Historical Perspective

Self-modeling curve resolution by factor analysis of a continuous series of pyrolysis mass spectra

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