Pyroptosis: A new insight of non-small-cell lung cancer treatment

Chen, Xi; Wu, Jianzhang; Wang, Jiabing

doi:10.3389/fonc.2022.1013544

Cited by 3 publications

(1 citation statement)

References 119 publications

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“…GSDMD would form GSDMD-N-terminal (GSDMD-NT) and GSDMD-C-terminal (GSDMD-CT) to form pores. IL-1β, interleukin-18 (IL-18), and cellular contents would be released into the extracellular space and in ammatory response would be activated [24] .…”

Section: Discussionmentioning

confidence: 99%

Identification of the prognosis value of pyroptosis related genes in the NSCLC

Huang,

Fan,

et al. 2024

Preprint

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Backgrounds:Our study aimed to identify the prognosis value of pyroptosis related genes of cancer associated fibroblasts (CAFs) in the NSCLC. Methods:We downloaded series matrix file data of GSE117570 from the NCBI GEO public database for single cell analysis. And the GSE30219, GSE37745 were used for verification. Furthermore, we downloaded the raw mRNA expression data of processed Lung adenocarcinoma (LUAD) including normal group (n=59), tumor group (n=541) from the TCGA database (https://portal.gdc.cancer.gov/).We performed gene set enrichment analysis (GSEA) and gene set variant analysis (GSVA) for the pyroptosis-related genes. Then we constructed the risk model using the pyroptosis related genes and identify the difference signature of the CAFs between the high risk group and low risk group. Nomogram and Kaplan–Meier method were used to validate the prognostic value of risk model. Results:We constructed a risk model using the GGA2, TLR2, PKM , CALM3, RBBP7, CAPNS1, YWHAZ and BIRC3. GSEA results showed that carbon metabolism, galactose metabolism, and p53 signaling pathways were all involved. The results of GSVA showed that the differences between patients were mainly enriched in signaling pathways such as mTORC1 signaling. The T cells CD8, Macrophages M0, Macrophages M1 were significantly higher in the high risk group. The expression levels of 8 model genes were significantly correlated with the expression levels of multiple tumor-related genes. BIRC3 and ATM are significantly positively correlated. Conclusions:The pyroptosis of the CAFs are related to the prognosis of the NSCLC.

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Section: Discussionmentioning

confidence: 99%

Identification of the prognosis value of pyroptosis related genes in the NSCLC

Huang,

Fan,

et al. 2024

Preprint

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High expression of PAFAH1B3 results in poor prognosis in lung adenocarcinoma patients and is associated with tumor cell pyroptosis genes

Hu,

Du,

Yuan

et al. 2024

CCB

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Background Lung adenocarcinoma (LUAD) is a common cancer with a poor prognosis. Platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29 kDa (PAFAH1B3) plays an important role in the development of many types of human malignancies. However, the precise role and mechanisms of PAFAH1B3 in LUAD are still unknown.Therefore, we will initially explore the effect of PAFAH1B3 on LUAD in this study. Methods In this study, we first performed a pan-cancer analysis of PAFAH1B3 expression and prognosis using The Cancer Genome Atlas (TCGA), genotype-tissue expression (GTEx) data, and GEPIA database. Next, the relationship between PAFAH1B3 expression and LUAD immune infiltration and pyroptosis-related genes was explored by GEPIA database and TIMER database. The effect of PAFAH1B3 on LUAD was further explored by CCK-8, wound healing, and Transwell assays. Finally, non-coding RNA (ncRNA) that may be involved in the regulation of PAFAH1B3 was explored using Starbase database analysis. Results The results found that PAFAH1B3 may be an oncogene in LUAD and has a significant adverse relationship with tumor immune cell infiltration, immune cell biomarkers and pyroptosis-related gene expression. Meanwhile, cell experiments also found that PAFAH1B3 knockout significantly reduced the proliferation, migration and invasion of A549 cells. Conclusions PAFAH1B3 high expression in LUAD patients is associated with poor prognosis, tumor immune infiltration, and cell pyroptosis gene expression.

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