Pyrosequencing analysis of bacterial diversity in dental unit waterlines

Costa, Damien; Mercier, Anne; Gravouil, Kévin; Lesobre, J.; Delafont, Vincent; Bousseau, A.; Verdon, Julien; Imbert, Christophe

doi:10.1016/j.watres.2015.05.065

Cited by 35 publications

(44 citation statements)

References 42 publications

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“…The authors conclude that microbes in this community may infect patients or medical staff at any point of time and harm their health. These microbes are small droplets in an infectious bioaerosol, which develops during dental treatment (23,24).…”

Section: Discussionmentioning

confidence: 99%

Legionella spp. in dental unit waterlines

Jurásková¹,

Sedlackova²,

Janska³

et al. 2017

BLL

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OBJECTIVE: To determine the current presence of Legionella spp. in the output water of dental unit waterlines (DUWLs) and examine its mitigation by disinfection at the Institute of Dentistry and Oral Sciences, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc. MATERIAL AND METHODS: The fi rst stage of our survey involved collecting samples of DUWL output water from 50 dental chair units (DCUs), and 2 samples of the incoming potable water. In October 2015, a one-time disinfection (1 % Stabimed) of DUWLs was conducted. This was followed by collecting 10 control samples (survey stage 2). RESULTS: From the total of 50 samples (survey stage 1), 18 samples (36.0 %) tested positive for Legionella spp. Following the disinfection, nine of the ten samples no longer showed any presence of Legionella. CONCLUSION: Based on culture results, the one-time disinfection (1 % Stabimed) was effective. We are unable to comment on the duration of positive effect of disinfection on the occurrence of Legionella spp. in the outlet water. It was a one-time survey (Tab. 2, Ref. 32). Text in PDF www.elis.sk. KEY WORDS: Legionella spp., water of dental unit waterlines, disinfection.

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Section: Discussionmentioning

confidence: 99%

Legionella spp. in dental unit waterlines

Jurásková¹,

Sedlackova²,

Janska³

et al. 2017

BLL

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show abstract

“…As DUWL water is used in most dental treatments, it may be a potential source of infection for both staff and patients. Many studies have examined the bacterial contamination present in DUWLs but few have examined the types of microorganisms in detail (Barbeau et al, 1996;Costa et al, 2015;Szymanska and Sitkowska, 2013a;Walker et al, 2004;Yabune et al, 2008) . In addition, most of these studies have assessed the bacterial diversity of water from DUWLs using in vitro cultivation (Barbeau et al, 1996;Szymanska and Sitkowska, 2013a;Yabune et al, 2008) .…”

Section: Introductionmentioning

confidence: 99%

“…High-throughput DNA sequencing method have been successfully used ＊ in several microbiological studies (Chun et al, 2010;Holinger et al, 2014;Poza et al, 2012) . To date, there has only been one prior study that has used Highthroughput DNA sequencing to examine bacterial diversity in water from DUWLs (Costa et al, 2015) .…”

Section: Introductionmentioning

confidence: 99%

An Assessment of the Bacterial Diversity found in Dental Unit Waterlines using the Illumina MiSeq

Yoon

Lee

2019

Biocontrol Sci.

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“…Furthermore, the molecular techniques cannot detect enough pathogenic bacteria at a go, and are also unable to identify diversity and abundance of pathogenic bacteria in water. Recent technological advances in next-generation sequencing (NGS) offer better prospects for detection of pathogenic microorganisms and investigating their diversity [11]. NGS can quickly generate huge amounts of DNA reads, and the technique is affordable [12].…”

Section: Introductionmentioning

confidence: 99%