Pyrosequencing Reveals Significant Changes in Microbial Communities Along the Ecological Succession of Biological Soil Crusts in the Tengger Desert of China

Zhang, Qingyi; Wang, Qiong; Ouyang, Hailong; Lan, Shubin; Hu, Chunxiang

doi:10.1016/s1002-0160(17)60477-6

Cited by 28 publications

(25 citation statements)

References 57 publications

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“…Immediately after the samples reached the laboratory, the water content (WC) ( Zhang et al, 2018 ) and texture ( Olmstead et al, 1930 ) were measured. Electrical conductivity, pH, and Eh were measured in a soil suspension with a soil:water ratio of 1:5 (w/v).…”

Section: Methodsmentioning

confidence: 99%

Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession

et al. 2021

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The successional ecology of nitrogen cycling in biocrusts and the linkages to ecosystem processes remains unclear. To explore this, four successional stages of natural biocrust with five batches of repeated sampling and three developmental stages of simulated biocrust were studied using relative and absolute quantified multi-omics methods. A consistent pattern across all biocrust was found where ammonium assimilation, mineralization, dissimilatory nitrite to ammonium (DNiRA), and assimilatory nitrate to ammonium were abundant, while denitrification medium, N-fixation, and ammonia oxidation were low. Mathematic analysis showed that the nitrogen cycle in natural biocrust was driven by dissolved organic N and NO3–. pH and SO42– were the strongest variables affecting denitrification, while C:(N:P) was the strongest variable affecting N-fixation, DNiRA, nitrite oxidation, and dissimilatory nitrate to nitrite. Furthermore, N-fixation and DNiRA were closely related to elemental stoichiometry and redox balance, while assimilatory nitrite to ammonium (ANiRA) and mineralization were related to hydrological cycles. Together with the absolute quantification and network models, our results suggest that responsive ANiRA and mineralization decreased during biocrust succession; whereas central respiratory DNiRA, the final step of denitrification, and the complexity and interaction of the whole nitrogen cycle network increased. Therefore, our study stresses the changing environmental functions in the biocrust N-cycle, which are succession-dependent.

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Section: Methodsmentioning

confidence: 99%

Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession

et al. 2021

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“…7a), together with eukaryotic Fungi (Ascomycota) and Amoebozoa (Protosporangiida) (Fig. 7b) are likely responsible for the majority of soil OC respiration and CO 2 emissions (Zhang et al, 2018;Swenson et al 2018). Cyanobacterial abundance was very low in the salada soils, especially when compared to biocrusts and soils in other playas worldwide (e.g.…”

Section: Microbial Diversity and Compositionmentioning

confidence: 99%

Changes In Soil Properties Across A Hydrological Gradient In Saladas From Northeast Spain: Implications For Soil Carbon Stocks, CO2 Efflux And Microbial Communities In A Warming World.

Thomas

Tooth

Lan

et al. 2021

Preprint

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Numerous permanent and temporary wetlands occur throughout the world’s drylands. Although characterised by diverse hydroperiods, these wetlands in drylands are typically hotspots of biological activity and productivity. The healthy functioning and possibly even existence of many wetlands in drylands, however, is threatened by desiccation resulting from a combination of climate change and human disturbance. Near Alcañiz in arid northeast Spain, three adjacent saladas (playas) with contrasting hydroperiods provide an opportunity to investigate how moisture availability affects their soil carbon (C) stocks, CO2 efflux, and microbial communities. Frequent inundation and/or near-permanent soil saturation supports the generation of organic C from a range of different sources. Soil inorganic C was greatest on the driest salada (3.8 %) compared to the wetter saladas (3.0 % and 2.1 %) owing to evaporative concentration and the reaction of CO2 with available Ca2+, Mg2+ and Na+ ions. CO2 efflux was greatest at intermediate moisture levels (142 mg CO2 m-2 hr-1), but the spatial and temporal variability in CO2 efflux on salada surfaces is very high, demonstrating the need for intensive sampling regimes to provide realistic estimates of their contribution to atmospheric CO2 exchanges. Different microbial community structures also characterise each salada. The saladas near Alcañiz, and many other similar features in northeast Spain, are renowned for their rare and threatened flora and fauna, yet their soil C cycle characteristics and soil microbial communities provide additional reasons to monitor the impacts of climate change and protect these vulnerable environments from further anthropogenic disturbances.

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“…Total DNA was extracted from the BSCs with Mag-Bind Soil DNA Kit (OMEGA, USA) following the manufacturer's instruction, and 16S and 18S rRNA gene segments were PCR amplicated from each sample DNA according to the method of Zhang et al [22]. The amplicons were used for pyrosequencing analysis on a Roche GS FLX Titanium machine (Roche, USA), which was carried out by Majorbio Biotech Co. Ltd.…”

Section: Crust Pyrosequencing Data Analysismentioning

confidence: 99%

“…The taxonomic annotation information of each OTU were then extracted from the SILVA SSU rRNA database. Although the microbial community in the BSCs has been analyzed at phylum level by Zhang et al [22], microalgal composition is still unknown. Therefore, in this study the same pyrosequencing data were used to analyze microalgal composition in the BSCs from Shapotou region.…”

Section: Crust Pyrosequencing Data Analysismentioning

confidence: 99%

“…The BSCs in our experimental regions mainly include cyanobacterial, lichen and moss soil crusts, and average 20471 and 21391 reads per sample have been obtained for prokaryotic and eukaryotic microbes, respectively [22]. Based on the pyrosequencing data, the OTUs for prokaryotic cyanobacteria and eukaryotic green algae and diatom were drawn out from the original crust microbial communities.…”

Section: Microalgal Composition In Bscsmentioning

confidence: 99%

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Resource utilization of microalgae from biological soil crusts: Biodiesel production associated with desertification control

Lan

Zhang

et al. 2018

Biomass and Bioenergy

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With the continuing consumption of resources and increasingly prominent environmental issues, microalgal resource utilization has received extensive attention. In this study, based on the microalgal investigation in desert biological soil crusts (BSCs) using pyrosequencing technology, the cultivated crust microalgae were further isolated in order to obtain high quality microalgae for resource utilization. The results environments, but also provide ideal places for the local microalgal resource exploitation, further promoting desert socioeconomic development.

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Pyrosequencing Reveals Significant Changes in Microbial Communities Along the Ecological Succession of Biological Soil Crusts in the Tengger Desert of China

Cited by 28 publications

References 57 publications

Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession

Metagenomic Insight Into Patterns and Mechanism of Nitrogen Cycle During Biocrust Succession

Changes In Soil Properties Across A Hydrological Gradient In Saladas From Northeast Spain: Implications For Soil Carbon Stocks, CO2 Efflux And Microbial Communities In A Warming World.

Resource utilization of microalgae from biological soil crusts: Biodiesel production associated with desertification control

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