Pyrrolidine Dithiocarbamate Enhances the Cytotoxic Effect of Arsenic
Trioxide on Acute Promyelocytic Leukemia Cells

Han, Jinbin; Yu, Simin; Ge, Zhuowang; Chen, Weixiang

doi:10.2174/1386207326666230123155944

Cited by 2 publications

(3 citation statements)

References 34 publications

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“…Для прикладу може порушитись детоксикація ксенобіотичних сполук у печінці внаслідок зниження активності процесів мікросомального окиснення, де для знешкодження ксенобіотиків застосовуються активні форми кисню (АФК) [4]. Високоселективні інгібітори активації транскрипційного фактора NF-κB, такі як амоній піролідиндитіокарбамат (ПДТК), мають здатність індукувати апоптоз та зупиняти клітинний цикл [5]. Зупинка клітинного циклу під впливом блокаторів активації транскрипційного фактора NF-κB може знизити здатність тканин до регенерації, що особливо негативно вплине на бар'єрні тканини (шкіра, слизові оболонки) та кістковий мозок.…”

Section: вступunclassified

“…Мигдалики розрізали ножицями і промивали середовищем, вимиваючи клітини. Процедуру повторювали декілька разів (5)(6)(7), після цього фільтрували через нейлон. Усі маніпуляції проводили у ламінарній шафі в потоці стерильного повітря.…”

Section: літератураunclassified

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Effect of Ammonium Pyrrolidinedithiocarbamate on the Development of Oxidative Stress in Biceps Femoris Muscles of Rats During Modelled Metabolic Syndrome

Akimov,

Mykytenko,

Mischenko

et al. 2024

Act. Probl. of the Modern Med.

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Excessive consumption of high-calorie food, a sedentary lifestyle and increased psycho-emotional stress are risk factors for the development of metabolic syndrome. These factors are especially relevant for residents of countries with highly developed economies. Metabolic syndrome is accompanied not only by metabolic disorders, but also leads to the development of a systemic inflammatory response, which is associated with excessive production and circulation of cytokines in the blood. As a rule, the activation of the transcription factor NF-κB leads to an increase in the production of pro-inflammatory cytokines. The aim of this work is to determine the effect of the NF-κB transcription factor activation inhibitor on the activity of antioxidant enzymes, the production of superoxide anion radical, the content of oxidatively modified proteins, and the concentration of malondialdehyde in the biceps femoris muscle of rats who underwent experimental metabolic syndrome. The study included 24 sexually mature male Wistar rats weighing 200-260 g. The animals were divided into 4 groups of 6 animals each. The first group was the control group; the second group involved the rats exposed to metabolic syndrome simulated by adding a 20% fructose solution as the only source of drinking water to the standard vivarium diet for 60 days; the third group involved the animals received ammonium pyrrolidine dithiocarbamate in a dose of 76 mg/kg intraperitoneally 3 times a week for 60 days; the fourth group was exposed to the combined effect of the ammonium pyrrolidinedithiocarbamate administration during modeled metabolic syndrome. In this study we investigated a 10% homogenate of the biceps femoris muscle with the research focus on several parameters: superoxide anion radical production, superoxide dismutase and catalase activity, malondialdehyde concentration, and the presence of oxidatively modified proteins. Simulation of the metabolic syndrome led to the development of oxidative stress in the biceps muscle of rats that was accompanied by an increase in the production of the superoxide anion radical and a decrease in the activity of antioxidant enzymes. The administration of ammonium pyrrolidinedithiocarbamate during modeled metabolic syndrome caused a decrease in the basic production of superoxide anion radical, production of superoxide anion radical by the microsomal electron transport chain and production of superoxide anion radical by the mitochondrial electron transport chain by 30.28%, 26.21 %, and 27.00%, respectively, compared to the rats in metabolic syndrome group. Superoxide dismutase activity increased by 78.81% and catalase activity grew by 144.74% compared to the metabolic syndrome group. The concentration of free malondialdehyde under the administration of ammonium pyrrolidinedithiocarbamate during metabolic syndrome reduced by 51.80%, and the content of oxidatively modified proteins decreased by 31.74% compared to the metabolic syndrome group. The administration of ammonium pyrrolidinedithiocarbamate, an NF-κB transcription factor activation inhibitor, effectively prevents the development of oxidative stress in the biceps femoris muscle of rats with modeled metabolic syndrome. This study is a part of the initiative research project No. 0124U000092 "High- and low-intensity phenotypes of systemic inflammatory response: molecular mechanisms and new medical technologies for their prevention and correction".

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Section: вступunclassified

Section: літератураunclassified

Effect of Ammonium Pyrrolidinedithiocarbamate on the Development of Oxidative Stress in Biceps Femoris Muscles of Rats During Modelled Metabolic Syndrome

Akimov,

Mykytenko,

Mischenko

et al. 2024

Act. Probl. of the Modern Med.

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“…The bioactive compounds of TCM serve as the fundamental basis for its pharmacological efficacy, exhibiting commendable clinical performance in the AML treatment. Arsenic trioxide (ATO) is one of the most common drugs used in the frontline treatment of APL that act through targeting and destabilizing the PML/RARα oncofusion protein ( Yu et al, 2023 ). ATO together with all-trans retinoic acid (ATRA) lead to durable remission of more than 90% non-high-risk APL patients, turning APL treatment into a paradigm of oncoprotein targeted cure ( Langdon et al, 2024 ).…”

Section: Bioactive Compounds In Tcm Targeting Epigenetic Mechanisms I...mentioning

confidence: 99%

Traditional Chinese medicine for acute myelocytic leukemia therapy: exploiting epigenetic targets

Gao,

Zuo,

Min

et al. 2024

Front. Pharmacol.

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Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy with historically high mortality rates. The treatment strategies for AML is still internationally based on anthracyclines and cytarabine, which remained unchanged for decades. With the rapid advance on sequencing technology, molecular targets of leukemogenesis and disease progression related to epigenetics are constantly being discovered, which are important for the prognosis and treatment of AML. Traditional Chinese medicine (TCM) is characterized by novel pharmacological mechanisms, low toxicity and limited side effects. Several biologically active ingredients of TCM are effective against AML. This review focuses on bioactive compounds in TCM targeting epigenetic mechanisms to address the complexities and heterogeneity of AML.

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Pyrrolidine Dithiocarbamate Enhances the Cytotoxic Effect of Arsenic Trioxide on Acute Promyelocytic Leukemia Cells

Cited by 2 publications

References 34 publications

Effect of Ammonium Pyrrolidinedithiocarbamate on the Development of Oxidative Stress in Biceps Femoris Muscles of Rats During Modelled Metabolic Syndrome

Effect of Ammonium Pyrrolidinedithiocarbamate on the Development of Oxidative Stress in Biceps Femoris Muscles of Rats During Modelled Metabolic Syndrome

Traditional Chinese medicine for acute myelocytic leukemia therapy: exploiting epigenetic targets

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