Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence

Abstract

The aim of this study was to investigate the effects of pyrroloquinoline quinone (PQQ), an oxidoreductase cofactor, on the H2O2-induced premature senescence model in HEI-OC1 auditory cells and to elucidate its mechanism of action in vitro. Cells were treated with PQQ for 1 day before H2O2 (100 μM) exposure. Mitochondrial respiratory capacity was damaged in this premature senescence model but was restored in cells pretreated with PQQ (0.1 nM or 1.0 nM). A decrease in mitochondrial potential, the promotion of mitochondrial fusion and the accelerated movement of mitochondria were all observed in PQQ-pretreated cells. The protein expression of sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) were significantly decreased under H2O2 exposure while they were increased with PQQ pretreatment, and PGC-1α acetylation was significantly decreased. In conclusion, PQQ has a protective effect on the premature senescence model of HEI-OC1 auditory cells and is associated with the SIRT1/PGC-1α signaling pathway, mitochondrial structure, and mitochondrial respiratory capacity.

Protective role of pyrroloquinoline quinone against gentamicin induced cochlear hair cell ototoxicity

“…The anterograde mitochondrial transport delivers healthy mitochondria to peripheral sites whereas the retrograde mitochondrial transport returns damaged mitochondria to central sites, which is crucial for neural survival (Schwarz, 2013). Mitochondrial motility and dynamics both regulate and reflect mitochondrial function in auditory cells (Gao et al, 2022). The recovery of mitochondrial dynamics is an important step in the protection of mitochondrial metabolic activity in auditory cells (Zou et al, 2022).…”

Effects of pyrroloquinoline quinone on noise-induced and age-related hearing loss in mice

“…It is known that the SV is most commonly affected by mitochondrial dysfunction, followed by the HCs and SGCs 38 . In addition, the mitochondrial function in House Ear Institute-Organ of Corti 1 (HEI-OC1) auditory cell lines was protected by PQQ pretreatment in an H 2 O 2 -induced senescence model and is associated with the SIRT1/PGC-1α signaling pathway 39 . The protective effect of PQQ in mitochondrial biogenesis is also expected in inner ear tissue, suggesting that PQQ can also work protectively in the auditory organs.…”

Pyrroloquinoline Quinone (PQQ): a novel approach to managing chronic inflammatory response syndrome and mitochondrial dysfunction

“…Pyrroloquinoline quinone (PQQ) is an antioxidant and a cofactor for certain enzymes and known for its potential neuroprotective, anti-inflammatory, and mitochondria-supporting properties. [24][25][26] Nutraceuticals have emerged as a promising avenue for addressing mitochondrial dysfunction and related health issues. 27 PQQ is a redox cofactor that has garnered significant attention for its potential to improve mitochondrial function and alleviate age-related conditions.…”